The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media
Abstract
The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40 degrees C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
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“In contrast, hardwoods and softwoods may require prohibitive quantities of energy to grind to fine powders.183,186”
From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [186]
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Metadata
- Authors
- Kamiya, N., Matsushita, Y., Hanaki, M., Nakashima, K., Narita, M., Goto, M., & Takahashi, H.
- Affiliations
- Kyushu University (JP)
- Toyota Central Research and Development Laboratories (Japan) (JP)
- Corresponding author
- Kamiya, Noriho
- Venue
- Biotechnology Letters
- Published
- 2008-02-05
- Volume
- 30
- Issue
- 6
- Pages
- 1037-1040
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2043267151
- Cited by
- 207
- References
- 12
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 8 · 2019: 8 · 2018: 10 · 2017: 17 · 2016: 22 · 2015: 14 · 2014: 13 · 2013: 20 · 2012: 21
- FWCI
- 11.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Ionic liquids properties and applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- enzymatic saccharification, cellulose, ionic liquids, cellulase, Avicel PH 101, glucose production, cellobiose, citrate buffer
- MeSH terms
- Cellobiose, Cellulase, Cellulose, Enzyme Activation, Glucose, Imidazoles, Ionic Liquids
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