Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step
Abstract
Hydrolysis of cellulose to glucose in aqueous media catalyzed by the cellulase enzyme system suffers from slow reaction rates due in large part to the highly crystalline structure of cellulose and inaccessibility of enzyme adsorption sites. In this study, an attempt was made to disrupt the cellulose structure using the ionic liquid (IL), 1‐ n ‐butyl‐3‐methylimidazolium chloride, in a cellulose regeneration strategy which accelerated the subsequent hydrolysis reaction. ILs are a new class of non‐volatile solvents that exhibit unique solvating properties. They can be tuned to dissolve a wide variety of compounds including cellulose. Because of their extremely low volatility, ILs are expected to have minimal environmental impact on air quality compared to most other volatile solvent systems. The initial enzymatic hydrolysis rates were approximately 50‐fold higher for regenerated cellulose as compared to untreated cellulose (Avicel PH‐101) as measured by a soluble reducing sugar assay. © 2006 Wiley Periodicals, Inc.
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Several empirical and semi-empirical polarity scales have been used to explain and predict solubility of biomolecules and biopolymers in solvents, for example Hansen solubility parameters, COSMO-RS and Kamlet-Taft polarity parameters.
“Several empirical and semi-empirical polarity scales have been used to explain and predict solubility of biomolecules and biopolymers in solvents, for example the Hansen solubility parameters, 21,85 COSMO-RS 86 and the Kamlet-Taft polarity parameters.87”
From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [85]
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- Authors
- Dadi, A. P., Varanasi, S., & Schall, C. A.
- Affiliations
- University of Toledo (US)
- Corresponding author
- Schall, Constance A.
- ORCID
- Schall, Constance A.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2006-08-17
- Volume
- 95
- Issue
- 5
- Pages
- 904-910
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2169991758
- Cited by
- 574
- References
- 39
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 10 · 2022: 16 · 2021: 25 · 2020: 32 · 2019: 26 · 2018: 34 · 2017: 28 · 2016: 28 · 2015: 33 · 2014: 39 · 2013: 56 · 2012: 67
- FWCI
- 13.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Materials Science › Biomaterials › Advanced Cellulose Research Studies
- Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
- Keywords
- cellulose hydrolysis, ionic liquid pretreatment, 1-butyl-3-methylimidazolium chloride, cellulose regeneration, enzymatic hydrolysis, cellulase, hydrolysis kinetics, cellulose crystallinity, Avicel PH 101, reducing sugars
- MeSH terms
- Cellulose, Glucose, Hydrolysis, Imidazoles, Chemical Precipitation, Ionic Liquids
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