The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Nanosecond Electric Pulses: A Novel Stimulus for Triggering Ca2+ Influx into Chromaffin Cells Via Voltage-Gated Ca2+ Channels
Open accessAbstract
Exposing bovine chromaffin cells to a single 5 ns, high-voltage (5 MV/m) electric pulse stimulates Ca(2+) entry into the cells via L-type voltage-gated Ca(2+) channels (VGCC), resulting in the release of catecholamine. In this study, fluorescence imaging was used to monitor nanosecond pulse-induced effects on intracellular Ca(2+) level ([Ca(2+)](i)) to investigate the contribution of other types of VGCCs expressed in these cells in mediating Ca(2+) entry. ω-Conotoxin GVIA and ω-agatoxin IVA, antagonists of N-type and P/Q-type VGCCs, respectively, reduced the magnitude of the rise in [Ca(2+)](i) elicited by a 5 ns pulse. ω-conotoxin MVIIC, which blocks N- and P/Q-type VGCCs, had a similar effect. Blocking L-, N-, and P\Q-type channels simultaneously with a cocktail of VGCC inhibitors abolished the pulse-induced [Ca(2+)](i) response of the cells, suggesting Ca(2+) influx occurs only via VGCCs. Lowering extracellular K(+) concentration from 5 to 2 mM or pulsing cells in Na(+)-free medium suppressed the pulse-induced rise in [Ca(2+)](i) in the majority of cells. Thus, both membrane potential and Na(+) entry appear to play a role in the mechanism by which nanoelectropulses evoke Ca(2+) influx. However, activation of voltage-gated Na(+) channels (VGSC) is not involved since tetrodotoxin (TTX) failed to block the pulse-induced rise in [Ca(2+)](i). These findings demonstrate that a single electric pulse of only 5 ns duration serves as a novel stimulus to open multiple types of VGCCs in chromaffin cells in a manner involving Na(+) transport across the plasma membrane. Whether Na(+) transport occurs via non-selective cation channels and/or through lipid nanopores remains to be determined.
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“The results from electroporation studies further support that ion channel activation and ion influx, and perhaps other effects such as cytoskeleton damage, can be used as biomarkers of responsiveness to TTFields, given that these phenomena are observed after exposure to electric fields [27,46,62,65].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [65]
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“After the first threshold is surpassed, it is thought that the Vm depolarizes the membrane sufficiently to trigger the formation of aqueous pores and stimulate other voltage-dependent processes such as ion channel activation and cytoskeletal disruption [62,65].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [65]
In the citing paper
“This study concluded that 5-ns duration, high-intensity (50,000 V/cm) electric pulses were sufficient to open up the L-type Cav2+ channels, resulting in detectable calcium influx [65].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [65]
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Citations
Cites
Ion Channels/Excitable Membranes
(2011) · Springer eBooks · doi:10.1007/springerreference_33998 · Cited by 4,066
A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine
Fenwick, E. M., Marty, A., & Neher, E. (1982) · The Journal of Physiology · doi:10.1113/jphysiol.1982.sp014393 · Cited by 812
Intracellular effect of ultrashort electrical pulses
Schoenbach, K. H., Beebe, S. J., & Buescher, E. S. (2001) · Bioelectromagnetics · doi:10.1002/bem.71 · Cited by 548
Chang, D. C., & Reese, T. S. (1990) · Biophysical Journal · doi:10.1016/s0006-3495(90)82348-1 · Cited by 524
Membrane Electroporation: A Molecular Dynamics Simulation
Tarek, M. (2005) · Biophysical Journal · doi:10.1529/biophysj.104.050617 · Cited by 516
The molecular basis of electroporation
Tieleman, D. P. (2004) · BMC Biochemistry · doi:10.1186/1471-2091-5-10 · Cited by 460
Calcium bursts induced by nanosecond electric pulses
Vernier, P. T., Sun, Y. H., Marcu, L., Salemi, S., Craft, C. M., & Gundersen, M. A. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.08.140 · Cited by 400
Self-citationCalcium signaling and exocytosis in adrenal chromaffin cells
García, A. G., García-De-Diego, A. M., Gandía, L., Borges, R., & García-Sancho, J. (2006) · Physiological Reviews · doi:10.1152/physrev.00039.2005 · Cited by 349
Nanoelectropulse-driven membrane perturbation and small molecule permeabilization
Vernier, P. T., Sun, Y. H., & Gundersen, M. A. (2006) · BMC Cell Biology · doi:10.1186/1471-2121-7-37 · Cited by 320
Self-citationPakhomov, A. G., Kolb, J. F., White, J. A., Joshi, R. P., Xiao, S. H., & Schoenbach, K. H. (2007) · Bioelectromagnetics · doi:10.1002/bem.20354 · Cited by 294
Cited by
Sorted by citations
Membrane Electroporation and Electropermeabilization: Mechanisms and Models
Kotnik, T., Rems, L., Tarek, M., & Miklavčič, D. (2019) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-052118-115451 · Cited by 813
Pall, M. L. (2013) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.12088 · Cited by 440
Napotnik, T. B., Reberšek, M., Vernier, P. T., Mali, B. Č., & Damijan Miklavčič, A. (2016) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2016.02.011 · Cited by 195
Self-citationOxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media
Pakhomova, O. N., Алексеевна, Х. В., Bowman, A. M., Rodaitė‐Riševičienė, R., Saulis, G., Xiao, S. H., & Pakhomov, A. G. (2012) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2012.08.004 · Cited by 189
In vitro electroporation detection methods – An overview
Napotnik, T. B., & Damijan Miklavčič, A. (2017) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2017.12.005 · Cited by 189
Metadata
- Authors
- Craviso, G. L., Choe, S., Chatterjee, P., Chatterjee, I., & Vernier, P. T.
- Affiliations
- University of Nevada, Reno (US)
- University of Southern California (US)
- Corresponding author
- Craviso, Gale L.
- Venue
- Cellular and Molecular Neurobiology
- Published
- 2010-11
- Volume
- 30
- Issue
- 8
- Pages
- 1259-1265
- ISSN
- 0272-4340 (ISSN-L)
- 1573-6830
- PMC
- PMC11498812
- OpenAlex
- W2003802737
- Cited by
- 107
- References
- 32
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 3 · 2023: 10 · 2022: 6 · 2021: 9 · 2020: 7 · 2019: 6 · 2018: 12 · 2017: 13 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 5 · 2012: 6
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cellular and Molecular Neurobiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agence Nationale pour la Gestion des Déchets Radioactifs
- Air Force Office of Scientific Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Keywords
- nanosecond pulsed electric fields, chromaffin cells, voltage-gated calcium channels, L-type calcium channels, N-type calcium channels, P/Q-type calcium channels, intracellular calcium influx, catecholamine release, omega-conotoxin GVIA, omega-agatoxin IVA, omega-conotoxin MVIIC, sodium transport, membrane potential, tetrodotoxin
- MeSH terms
- Animals, Calcium, Calcium Channel Blockers, Cattle, Electricity, Extracellular Space, Membrane Potentials, Sodium, Time Factors, Calcium Channels, Ion Channel Gating, Chromaffin Cells, Calcium Signaling
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