Cited by 107Ion channel regulation and functionMicrobial Inactivation MethodsIon Channels and Receptors

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Nanosecond Electric Pulses: A Novel Stimulus for Triggering Ca2+ Influx into Chromaffin Cells Via Voltage-Gated Ca2+ Channels

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Craviso, Gale L., Choe, Sophie, Chatterjee, Paroma, Chatterjee, Indira, Vernier, P. Thomas·2010·Cellular and Molecular Neurobiology

DOI 10.1007/s10571-010-9573-1PMC PMC11498812

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Abstract

Exposing bovine chromaffin cells to a single 5 ns, high-voltage (5 MV/m) electric pulse stimulates Ca(2+) entry into the cells via L-type voltage-gated Ca(2+) channels (VGCC), resulting in the release of catecholamine. In this study, fluorescence imaging was used to monitor nanosecond pulse-induced effects on intracellular Ca(2+) level ([Ca(2+)](i)) to investigate the contribution of other types of VGCCs expressed in these cells in mediating Ca(2+) entry. ω-Conotoxin GVIA and ω-agatoxin IVA, antagonists of N-type and P/Q-type VGCCs, respectively, reduced the magnitude of the rise in [Ca(2+)](i) elicited by a 5 ns pulse. ω-conotoxin MVIIC, which blocks N- and P/Q-type VGCCs, had a similar effect. Blocking L-, N-, and P\Q-type channels simultaneously with a cocktail of VGCC inhibitors abolished the pulse-induced [Ca(2+)](i) response of the cells, suggesting Ca(2+) influx occurs only via VGCCs. Lowering extracellular K(+) concentration from 5 to 2 mM or pulsing cells in Na(+)-free medium suppressed the pulse-induced rise in [Ca(2+)](i) in the majority of cells. Thus, both membrane potential and Na(+) entry appear to play a role in the mechanism by which nanoelectropulses evoke Ca(2+) influx. However, activation of voltage-gated Na(+) channels (VGSC) is not involved since tetrodotoxin (TTX) failed to block the pulse-induced rise in [Ca(2+)](i). These findings demonstrate that a single electric pulse of only 5 ns duration serves as a novel stimulus to open multiple types of VGCCs in chromaffin cells in a manner involving Na(+) transport across the plasma membrane. Whether Na(+) transport occurs via non-selective cation channels and/or through lipid nanopores remains to be determined.

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(2011) · Springer eBooks · doi:10.1007/springerreference_33998 · Cited by 4,066
A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine
Fenwick, E. M., Marty, A., & Neher, E. (1982) · The Journal of Physiology · doi:10.1113/jphysiol.1982.sp014393 · Cited by 812
Intracellular effect of ultrashort electrical pulses
Schoenbach, K. H., Beebe, S. J., & Buescher, E. S. (2001) · Bioelectromagnetics · doi:10.1002/bem.71 · Cited by 548
Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy
Chang, D. C., & Reese, T. S. (1990) · Biophysical Journal · doi:10.1016/s0006-3495(90)82348-1 · Cited by 524
Membrane Electroporation: A Molecular Dynamics Simulation
Tarek, M. (2005) · Biophysical Journal · doi:10.1529/biophysj.104.050617 · Cited by 516
The molecular basis of electroporation
Tieleman, D. P. (2004) · BMC Biochemistry · doi:10.1186/1471-2091-5-10 · Cited by 460
Calcium bursts induced by nanosecond electric pulses
Vernier, P. T., Sun, Y. H., Marcu, L., Salemi, S., Craft, C. M., & Gundersen, M. A. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.08.140 · Cited by 400
Self-citation
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García, A. G., García-De-Diego, A. M., Gandía, L., Borges, R., & García-Sancho, J. (2006) · Physiological Reviews · doi:10.1152/physrev.00039.2005 · Cited by 349
Nanoelectropulse-driven membrane perturbation and small molecule permeabilization
Vernier, P. T., Sun, Y. H., & Gundersen, M. A. (2006) · BMC Cell Biology · doi:10.1186/1471-2121-7-37 · Cited by 320
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Pakhomov, A. G., Kolb, J. F., White, J. A., Joshi, R. P., Xiao, S. H., & Schoenbach, K. H. (2007) · Bioelectromagnetics · doi:10.1002/bem.20354 · Cited by 294

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5 ns electric pulses induce Ca2+-dependent exocytotic release of catecholamine from adrenal chromaffin cells
Zaklit, J., Cabrera, A., Shaw, A. J., Aoun, R., Vernier, P. T., Leblanc, N., & Craviso, G. L. (2021) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2021.107830 · Cited by 8
Self-citation
Ion channels as molecular targets of glioblastoma electrotherapy
Abed, T., Ganser, K., Eckert, F., Stransky, N., & Huber, S. M. (2023) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2023.1133984 · Cited by 7
Numerical Study on Calcium Transport Through Voltage-Gated Calcium Channels in Response to Nanosecond Pulsed Electric Field
Bo, W., Tang, J., Ma, J., & Gong, Y. (2018) · IEEE Transactions on Plasma Science · doi:10.1109/tps.2018.2840324 · Cited by 7
Electric Fields Regulate In Vitro Surface Phosphatidylserine Exposure of Cancer Cells via a Calcium-Dependent Pathway
Kaynak, A., N’Guessan, K. F., Patel, P. H., Lee, J. H., Kogan, A. B., Narmoneva, D. A., & Qi, X. (2023) · Biomedicines · doi:10.3390/biomedicines11020466 · Cited by 7
The influence of asymmetrical bipolar pulses and interphase intervals on the bipolar cancellation phenomenon in the ovarian cancer cell line
Łapińska, Z., Novickij, V., Rembiałkowska, N., Szewczyk, A., Dubińska–Magiera, M., Kulbacka, J., & Saczko, J. (2023) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2023.108483 · Cited by 7
ns Pulsed Electric Field-Induced Action Potentials in the Circuital Model of an Axon
Lamberti, P., Compitiello, M., & Romeo, S. (2018) · IEEE Transactions on NanoBioscience · doi:10.1109/tnb.2018.2822840 · Cited by 7
Communicating with Mouse Oocytes via Regulating Calcium Oscillation Patterns by Nanosecond Pulsed Electric Fields
Liu, J., Lu, Q., Liang, R., Guo, J., Wang, K., Dong, F., Wang, J., Zhang, J., & Fang, J. (2019) · Physical Review Applied · doi:10.1103/physrevapplied.11.024001 · Cited by 7
Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
Napotnik, T. B., & Damijan Miklavčič, A. (2021) · Molecules · doi:10.3390/molecules26216571 · Cited by 6
Protein-Mediated Electroporation in a Cardiac Voltage-Sensing Domain Due to an nsPEF Stimulus
Ruiz-Fernández, A. R., Campos, L., Villanelo, F., Gárate, J. A., & Pérez‐Acle, T. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241411397 · Cited by 6
Modulating Ca2+ influx into adrenal chromaffin cells with short-duration nanosecond electric pulses
Yun, S. H., Mansurov, V., Yang, L., Yoon, J., Leblanc, N., Craviso, G. L., & Zaklit, J. (2024) · Biophysical Journal · doi:10.1016/j.bpj.2024.06.021 · Cited by 6
Self-citation
Pulse Power Ablation of Melanoma with Nanosecond Pulsed Electric Fields
Stephen, J., Wentia, E., Ren, W., & Che, X. (2011) · InTech eBooks · doi:10.5772/22850 · Cited by 6
Safety factor for electrostimulation with nanosecond pulses
Zemlin, C. W. (2021) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2021.107882 · Cited by 7
Electric pulse shape impact on biological effects: A modeling study
Garner, A. L., Maciejewski, J. J., Vadlamani, A., & Byer, R. J. (2015) · IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP) · doi:10.1109/ceidp.2015.7352148 · Cited by 6
High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca2+ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice
Viola, C., Gould, T. W., Procacci, N. M., Leblanc, N., Zaklit, J., & Craviso, G. L. (2023) · PLoS ONE · doi:10.1371/journal.pone.0283736 · Cited by 4
Self-citation
Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations
Napotnik, T. B., Cimperman, T., & Rems, L. (2025) · Scientific Reports · doi:10.1038/s41598-025-06989-5 · Cited by 3
Subcellular Biological Effects of Nanosecond Pulsed Electric Fields
Kolb, J. F., & Stacey, M. W. (2011) · NATO science for peace and security series. A, Chemistry and biology · doi:10.1007/978-94-007-2852-3_28 · Cited by 3
Microdosimetry applied to nanosecond pulsed electric fields: A comparison on a single cell between real and ideal waveforms
Merla, C., Paffi, A., Apollonio, F., Lévêque, P., & Liberti, M. (2011) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/iembs.2011.6090079 · Cited by 2
Caveolin-1 is Involved in Regulating the Biological Response of Cells to Nanosecond Pulsed Electric Fields
Cantu, J. C., Tolstykh, G. P., Tarango, M., Beier, H. T., & Ibey, B. L. (2021) · The Journal of Membrane Biology · doi:10.1007/s00232-020-00160-z · Cited by 2
Elementary Concepts and Definitions
Brosseau, C. (2023) · Studies in mechanobiology, tissue engineering and biomaterials · doi:10.1007/978-3-031-37981-9_2 · Cited by 2
Ultra short waves alleviate ulcerative colitis via TRPV2 mediated-macrophage polarization
Ning, Y., Wang, Y., Guo, M., Mai, J., Sun, W., Ge, Z., Han, K., Wang, C., Qin, L., Wang, X., et al. (2026) · iScience · doi:10.1016/j.isci.2026.115929 · Cited by 1
Extracellular calcium differentially affects intracellular responses to nanosecond pulsed electric fields
Morotomi‐Yano, K., & Yano, K. (2015) · Cited by 1
[Mechanism of ablation with nanosecond pulsed electric field].
Cen, C., Chen, X., & Zheng, S. (2015) · PubMed Central · doi:10.3785/j.issn.1008-9292.2015.11.13 · Cited by 1
Effects of usEPs on Plasma Membranes—Pores, Channels, and Repair
Beebe, S. J. (2021) · Series in bioengineering · doi:10.1007/978-981-10-5113-5_2 · Cited by 1
Application and Characterization Osteo Nanoporation under Electrical Stimuli
Sarkar, S., & Mk, G. (2015) · Gene Technology · doi:10.4172/2329-6682.1000123
The Application of Pulsed Electric Fields and Other Types of Electromagnetic Radiation in Therapy of Cancer
Shckorbatov, Y. (2018) · International Conference on Ultrawideband and Ultrashort Impulse Signals · doi:10.1109/uwbusis.2018.8520259
Protein‐mediated electroporation: Pore formation in a calcium voltage‐sensing domain induced by positive and negative nanosecond pulsed electric fields
Ruiz-Fernández, Á., Gallo-Méndez, I., Campos, L. R. S., Ceballos-Ulloa, F., Barria-Urenda, M., Garate, J. A., Araya-Secchi, R., & Pérez-Acle, T. (2025) · Protein Science · doi:10.1002/pro.70285
Na+ Uptake Triggered in Adrenal Chromaffin Cells by a 5-ns Electric Pulse is Facilitated by Ca2+ Influx Via an L-Type-Like Ca2+ Channel Independent of Changes in Membrane Potential
Yang, L., Zaklit, J., Gould, T. W., Craviso, G. L., & Leblanc, N. (2026) · The Journal of Membrane Biology · doi:10.1007/s00232-026-00370-x
Self-citation
Etude de la perméabilisation de la membrane plasmique et des membranes des organites cellulaires par des agents chimiques et physiques
de Ménorval, M. (2013) · theses.fr (ABES) · doi:10.70675/d1708e34z48b5z4d16zb301z89de603998a1
Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric fields : effective new tool to control mesenchymal stem cells spontaneous Ca2+ oscillations
Hanna, H. (2016) · theses.fr (ABES) · doi:10.70675/a848d0bez6431z4297zbbdbz7503ad283fef

Authors: Craviso, G. L., Choe, S., Chatterjee, P., Chatterjee, I., & Vernier, P. T.
Affiliations: University of Nevada, Reno (US)
University of Southern California (US)
Corresponding author: Craviso, Gale L.
ORCID: Craviso, Gale L.
Vernier, P. Thomas
Venue: Cellular and Molecular Neurobiology
Publisher: Springer Science and Business Media LLC
Published: 2010-11
Volume: 30
Issue: 8
Pages: 1259-1265
ISSN: 0272-4340 (ISSN-L)
1573-6830
DOI: 10.1007/s10571-010-9573-1
PMC: PMC11498812
OpenAlex: W2003802737
Cited by: 107
References: 32
Citations by year: 2026: 5 · 2025: 2 · 2024: 3 · 2023: 10 · 2022: 6 · 2021: 9 · 2020: 7 · 2019: 6 · 2018: 12 · 2017: 13 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 5 · 2012: 6
FWCI: 1.95
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Cellular and Molecular Neurobiology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Agence Nationale pour la Gestion des Déchets Radioactifs
Air Force Office of Scientific Research
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords: nanosecond pulsed electric fields, chromaffin cells, voltage-gated calcium channels, L-type calcium channels, N-type calcium channels, P/Q-type calcium channels, intracellular calcium influx, catecholamine release, omega-conotoxin GVIA, omega-agatoxin IVA, omega-conotoxin MVIIC, sodium transport, membrane potential, tetrodotoxin
MeSH terms: Animals, Calcium, Calcium Channel Blockers, Cattle, Electricity, Extracellular Space, Membrane Potentials, Sodium, Time Factors, Calcium Channels, Ion Channel Gating, Chromaffin Cells, Calcium Signaling

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Nanosecond Electric Pulses: A Novel Stimulus for Triggering Ca2+ Influx into Chromaffin Cells Via Voltage-Gated Ca2+ Channels

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