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Cited by 135Plant-Microbe Interactions and ImmunityFungal Plant Pathogen ControlMicrobial Natural Products and Biosynthesis

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Biological control of peach brown rot (Monilinia spp.) by Bacillus subtilis CPA-8 is based on production of fengycin-like lipopeptides

Yánez-Mendizábal, Viviana, Zeriouh, Houda, Viñas, Inmaculada, Torres, Rosario, Usall, Josep, de Vicente, Antonio, Pérez-García, Alejandro, Teixidó, Neus·2012·European Journal of Plant Pathology
DOI 10.1007/s10658-011-9905-0
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Abstract

Bacillus subtilis CPA-8, a strain with demonstrated ability to control Monilinia spp. in peaches, was studied to elucidate its mechanisms of antifungal activity. Growth inhibition assays using cell-free supernatants and butanolic extracts showed strong antifungal activities against Monilinia laxa and Monilinia fructicola. By comparison with the reference B. subtilis strains UMAF6614 and UMAF6639, fengycin, iturin and surfactin lipopeptides were identified by thin layer chromatography in butanolic extracts from cell-free supernatants, indicating that antibiosis could be a major factor involved in the biological control ability of CPA-8. TLC-bioautography analysis confirmed the presence of fengycin, iturin and surfactin lipopeptides but strong antifungal activity could be associated only with fengycin lipopeptides. These results were definitively supported by mutagenesis analysis targeted to suppress fengycin biosynthesis by disruption of the B. subtilis fenB gene. By TLC-bioautography analysis it was possible to identify transformants from CPA-8 with reduced or suppressed antifungal activity, and this phenotype was associated with the lack of fengycin bands. Fruit trials confirmed that fengycin-defective mutants and their cell-free supernatants lost their ability to control peach brown rot disease in comparison with CPA-8 wild type strain or Serenade Max®, a commercial formulation based on B. subtilis. Furthermore, population dynamics studies determined that CPA-8 fengycin-deficient mutants survived in wounds in peach fruit equally well as the CPA-8 wild type. Taken together our data indicate that fengycin-like lipopeptides play a major role in the biological control potential of B. subtilis CPA-8 against peach brown rot.

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  • Production of antifungal peptides (e.g. iturins, fengycins) has been frequently identified as a mode of action of several biological control agents, mainly bacteria, e.g. Bacillus spp.

    “For the control of postharvest diseases of horticultural produce, the production of antifungal peptides (e.g. iturins, fengycins, etc.) has been frequently identified as a mode of action of several biological control agents, mainly bacteria, e.g. Bacillus spp. (Yánez-Mendizábal et al., 2012; Waewthongrak et al., 2015).”

    From GRAS, plant- and animal-derived compounds as alternatives to conventional fungicides for the control of postharvest diseases of fresh horticultural produce (2016) · cites this paper as Yánez-Mendizábal et al., 2012

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Research works printed but not linked: 43

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Authors
Yánez-Mendizábal, V., Zeriouh, H., Viñas, I., Torres, R., Usall, J., de Vicente, A., Pérez-García, A., & Teixidó, N.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Institute of Agrifood Research and Technology (ES)
Corresponding author
Teixidó, Neus
ORCID
Venue
European Journal of Plant Pathology
Publisher
Springer Science and Business Media LLC
Published
2011-11-27
Volume
132
Issue
4
Pages
609-619
ISSN
  • 0929-1873 (ISSN-L)
  • 1573-8469
DOI
10.1007/s10658-011-9905-0
OpenAlex
W1984393545
Cited by
135
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 8 · 2023: 9 · 2022: 15 · 2021: 13 · 2020: 10 · 2019: 13 · 2018: 9 · 2017: 15 · 2016: 9 · 2015: 9 · 2014: 9 · 2013: 8
FWCI
6.93
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
European Journal of Plant Pathology (publishedVersion)
Funders
FP7 International Cooperation
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
Keywords
biological control, peach brown rot, Monilinia laxa, Monilinia fructicola, fengycin, lipopeptides, iturin, surfactin, antifungal activity, cell-free supernatant, wound colonization

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