Expression of S100A2 and S100P in human eccrine sweat glands and their application in differentiating secretory coil-like from duct-like structures in the 3D reconstituted eccrine sweat spheroids
Abstract
Secretory coils and ducts are two components of eccrine sweat glands with different structures and functions. In our previous study, we combined keratins and α-SMA to distinguish between secretory coils and ducts. However, the key deficiency of the method was that none of the antibodies used was specific for ducts. In this study, we first examined the co-localization of K5/K7, α-SMA/K14, K7/S100P and α-SMA/S100A2 by double-immunofluorescence staining to confirm the localization of S100P and S100A2 in native human eccrine sweat glands, and second we identified secretory coil-like and duct-like structures in the 3D reconstituted eccrine sweat gland spheroids by double-immunofluorescence staining for K7/S100P and α-SMA/S100A2. In native human eccrine sweat glands, S100A2 immunoreactivity was confined to the outer layer and S100P to the inner layer of the duct. In 12-week Matrigel plugs containing eccrine sweat gland cells, double-immunofluorescence staining for K7/S100P and α-SMA/S100A2 could easily distinguish duct-like structures from secretory coil-like structures. We conclude that S100A2 and S100P can be used as specific duct markers in eccrine sweat glands, and combined use of S100P or S100A2 with keratins enables easy to distinction between secretory coils and ducts.
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The 3D-reconstitution model by culturing Matrigel-embedded ESGCs in vitro or implanting them into the subcutis of nude mice simulates the development, morphology, and function of sweat glands in natural form.
“A recent study used a 3D-reconstitution model by culturing Matrigel-embedded eccrine sweat gland cells (ESGCs) in vitro or by implanting them into the subcutis of nude mice and concluded that this model simulates the development, morphology, and function of sweat glands in natural form.[12–16]”
From Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids (2022) · cites this paper as [13]
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Lu, C. P., Polak, L., Rocha, A. S., Pasolli, H. A., Chen, S.-C., Sharma, N., Blanpain, C., & Fuchs, E. (2012) · Cell · doi:10.1016/j.cell.2012.04.045 · Cited by 301 · see citing works
Langbein, L., Rogers, M. A., Praetzel, S., Cribier, B., Peltre, B., Gassler, N., & Schweizer, J. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23860.x · Cited by 72 · see citing works
Li, X., Li, H., Zhang, M., Chen, L., & Zhang, B. (2017) · Journal of Molecular Histology · doi:10.1007/s10735-017-9710-y · Cited by 9 · see citing works
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Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids
Du, L., Zhang, L., Zhao, J., Chen, Z., Liu, X., Cao, M., You, L., Zhang, Y., Fu, X., & Li, H. (2022) · Chinese Medical Journal · doi:10.1097/cm9.0000000000001936 · Cited by 6 · see citing works
Self-citationTime course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands
Zhang, M., Li, H., Xie, S., & Chen, L. (2018) · Journal of Molecular Histology · doi:10.1007/s10735-018-9795-y · Cited by 4 · see citing works
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- Authors
- Li, H., Zhang, M., Chen, L., Zhang, B., & Zhang, C.
- Affiliations
- Shantou University (CN)
- Shantou University Medical College (CN)
- First Affiliated Hospital of Chinese PLA General Hospital (CN)
- Corresponding author
- Li, Haihong
- Zhang, Cuiping
- Venue
- Journal of Molecular Histology
- Published
- 2017-03-28
- Volume
- 48
- Issue
- 3
- Pages
- 219-223
- ISSN
- 1567-2379 (ISSN-L)
- 1567-2387
- OpenAlex
- W2600690532
- Cited by
- 9
- References
- 3
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2020: 1 · 2018: 5
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Histology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81471882, 81071551, 81571905)
- he Natural Science Foundation of Guangdong Province, China (2014A030313476)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › S100 Proteins and Annexins
- Health Sciences › Medicine › Dermatology › Cancer and Skin Lesions
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Keywords
- S100A2, S100P, eccrine sweat glands, sweat ducts, keratin 7, keratin 5, alpha-smooth muscle actin, sweat gland differentiation
- MeSH terms
- Actins, Adolescent, Adult, Calcium-Binding Proteins, Cells, Cultured, Chemotactic Factors, Child, Child, Preschool, Collagen, Drug Combinations, Eccrine Glands, Fluorescent Antibody Technique, Humans, Keratins, Laminin, Neoplasm Proteins, S100 Proteins, Proteoglycans, Spheroids, Cellular, Young Adult
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