Cell proliferation and differentiation during the three dimensional reconstitution of eccrine sweat glands
Abstract
The aim of this study is to characterize the cell proliferation and proliferating cell types during three-dimensional reconstitution of eccrine sweat glands. Eccrine sweat gland cells suspended in Matrigel were injected subcutaneously into the inguinal regions of nude mice. At 1, 2, 4, 6, 8, 14, 21, 28, 35 and 42 days post-implantation, Matrigel plugs were immunostained for Ki67, to detect cycling cells, and the Ki67 labeling index at different time points was calculated. Three pairs of antibodies, Ki67/K7, Ki67/K14 and Ki67/α-SMA, were used to identify proliferating cell types in the plugs, on days 28, 35 and 42, by immunofluorescence double staining. The Ki67 labeling index on the first day of implantation was 30.53%, rapidly reached a peak value of 81.43% at 2 days post-implantation, and then decreased gradually to a low of 2.87% at 42 days. Double immunofluorescence staining showed that K14/Ki67 double-stained cells accounted for 80% of the Ki67-positive cells, whereas K7/Ki67 and α-SMA/Ki67 double-stained cells each accounted for 10% of the Ki67-positive population on days 28, 35, or 42 post-implantation. We conclude that eccrine sweat gland cells rapidly enter the cell cycle after implantation, but quickly show decreased cell proliferation and increased cell differentiation.
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The 3D-reconstitution model by culturing Matrigel-embedded ESGCs in vitro or implanting them into the subcutis of nude mice simulates the development, morphology, and function of sweat glands in natural form.
“A recent study used a 3D-reconstitution model by culturing Matrigel-embedded eccrine sweat gland cells (ESGCs) in vitro or by implanting them into the subcutis of nude mice and concluded that this model simulates the development, morphology, and function of sweat glands in natural form.[12–16]”
From Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids (2022) · cites this paper as [14]
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- Authors
- Li, X., Li, H., Zhang, M., Chen, L., & Zhang, B.
- Affiliations
- Shantou University Medical College (CN)
- Shantou University (CN)
- Corresponding author
- Li, Haihong
- Venue
- Journal of Molecular Histology
- Published
- 2017-01-16
- Volume
- 48
- Issue
- 2
- Pages
- 113-120
- ISSN
- 1567-2379 (ISSN-L)
- 1567-2387
- OpenAlex
- W2578728004
- Cited by
- 9
- References
- 32
- Citations by year
- 2024: 2 · 2022: 1 · 2018: 4 · 2017: 2
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Histology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81471882, 81071551)
- Natural Science Foundation of Guangdong Province, China (2014A030313476)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Dermatology › Cancer and Skin Lesions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- eccrine sweat glands, cell proliferation, cell differentiation, Ki-67 proliferation index, keratin 14, keratin 7, alpha-smooth muscle actin, nude mice, cell cycle
- MeSH terms
- Animals, Cell Cycle, Cell Differentiation, Cells, Cultured, Collagen, Drug Combinations, Eccrine Glands, Humans, Laminin, Mice, Nude, Proteoglycans, Time Factors, Ki-67 Antigen, Cell Proliferation, Mice
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