Time course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands
Abstract
Epidermal basal cells invaginate into the dermis to form sweat ducts, which then grow downwards further to form secretory coils during the ontogenesis of eccrine sweat glands, but the time course of differentiation of different cell types in 3D-reconstructed eccrine sweat glands remain unclear. In this study, secretory cell-specific marker K7, clear secretory cell-specific marker CA II, dark secretory cell-specific marker GCDFP-15, myoepithelial cell-specific marker α-SMA, inner duct cell-specific marker S100P and outer duct cell-specific marker S100A2 were detected by immunofluorescence staining. The results showed that S100P and S100A2 were first detected at 2 weeks post implantation, K7 and α-SMA at 3 weeks, and GCDFP-15 and CA II at 4 weeks. The differentiation of ducts preceded secretory coils in 3D-reconstructed eccrine sweat glands. After 8 weeks post implantation, the distribution of these markers in 3D-reconstructed eccrine sweat glands was similar to that in native ones, and the percentage of the 3D-reconstructed glands expressing these markers maintained steady. We conclude that although the 3D-reconstructed and native eccrine sweat glands originated from different cells, the differentiation of different cell types in 3D-reconstructed eccrine sweat glands parallels the sequence observed during embryonic development.
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The lumen structure in EGOs is similar to the primary structure of sweat gland ducts in body development.
“Therefore, we can infer that the lumen structure in our EGOs is similar to the primary structure of sweat gland ducts in body development.”
From Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids (2022) · cites this paper as [16]
The 3D-reconstitution model by culturing Matrigel-embedded ESGCs in vitro or implanting them into the subcutis of nude mice simulates the development, morphology, and function of sweat glands in natural form.
“A recent study used a 3D-reconstitution model by culturing Matrigel-embedded eccrine sweat gland cells (ESGCs) in vitro or by implanting them into the subcutis of nude mice and concluded that this model simulates the development, morphology, and function of sweat glands in natural form.[12–16]”
From Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids (2022) · cites this paper as [16]
Cites
Biology of sweat glands and their disorders. I. Normal sweat gland function
Sato, K., Kang, W. H., Saga, K., & Sato, K. T. (1989) · Journal of the American Academy of Dermatology · doi:10.1016/s0190-9622(89)70063-3 · Cited by 717 · see citing works
Lu, C. P., Polak, L., Rocha, A. S., Pasolli, H. A., Chen, S.-C., Sharma, N., Blanpain, C., & Fuchs, E. (2012) · Cell · doi:10.1016/j.cell.2012.04.045 · Cited by 301 · see citing works
Moll, R., Moll, I., & Wiest, W. (1982) · Differentiation · doi:10.1111/j.1432-0436.1982.tb01280.x · Cited by 229 · see citing works
Eccrine sweat gland development and sweat secretion
Cui, C., & Schlessinger, D. (2015) · Experimental Dermatology · doi:10.1111/exd.12773 · Cited by 213 · see citing works
Structure and function of human sweat glands studied with histochemistry and cytochemistry
Saga, K. (2002) · Progress in Histochemistry and Cytochemistry · doi:10.1016/s0079-6336(02)80005-5 · Cited by 190 · see citing works
Fu, X., Fang, L., Li, X., Cheng, B., & Sheng, Z. (2006) · Wound Repair and Regeneration · doi:10.1111/j.1743-6109.2006.00128.x · Cited by 159 · see citing works
van Muijen, G. N. P., Warnaar, S. O., & Ponec, M. (1987) · Experimental Cell Research · doi:10.1016/0014-4827(87)90166-2 · Cited by 158 · see citing works
Sweat Gland Progenitors in Development, Homeostasis, and Wound RepairPreprint
Lu, C. P.-J., & Fuchs, E. V. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a015222 · Cited by 156 · see citing works
BÖNI, R., Burg, G., Doguoglu, A., Ilg, E. C., Schäfer, B. W., Müller, B. P., & Heizmann, C. W. (1997) · British Journal of Dermatology · doi:10.1046/j.1365-2133.1997.17651853.x · Cited by 131 · see citing works
(2003) · Seminars in Neurology · doi:10.1055/s-2004-817724 · Cited by 105 · see citing works
Alpha-smooth muscle actin expression in cultured lens epithelial cells.
Nagamoto, T., Eguchi, G., & Beebe, D. C. (2000) · PubMed · Cited by 103 · see citing works
Cui, C., Childress, V., Piao, Y., Michel, M., Johnson, A. A., Kunisada, M., Ko, M. S. H., Kaestner, K. H., Marmorstein, A. D., & Schlessinger, D. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1117213109 · Cited by 80 · see citing works
Localization of Ca 2+ -binding S100 proteins in epithelial tumours of the skin
Shrestha, P., Muramatsu, Y., Kudeken, W., Mori, M., Takai, Y., Ilg, E. C., Schäfer, B. W., & Heizmann, C. W. (1998) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/s004280050134 · Cited by 78 · see citing works
Schön, M., Benwood, J., O’Connell, T. M., & Rheinwald, J. G. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.12.1925 · Cited by 70 · see citing works
Changes of Expression of Intermediate Filament Proteins During Ontogenesis of Eccrine Sweat Glands
Moll, I., & Moll, R. (1992) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12499950 · Cited by 61 · see citing works
Bovell, D. L., MacDonald, A., Meyer, B. A., Corbett, A. D., Maclaren, W. M., Holmes, S., & Harker, M. (2011) · Experimental Dermatology · doi:10.1111/j.1600-0625.2011.01361.x · Cited by 36 · see citing works
Expression of S100 protein family members in normal skin and sweat gland tumors
Zhu, L., Okano, S., Takahara, M., Chiba, T., Tu, Y., Oda, Y., & Furue, M. (2013) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2013.03.002 · Cited by 36 · see citing works
Li, H., Chen, L., Zeng, S., Li, X., Zhang, X., Lin, C., Zhang, M., Xie, S., He, Y., Shu, S., et al. (2015) · Experimental Cell Research · doi:10.1016/j.yexcr.2015.01.014 · Cited by 35 · see citing works
Self-citationVulvar hidradenitis suppurativa. Immunohistochemical evaluation of apocrine and eccrine involvement.
Heller, D. S., Haefner, H. K., Hameed, M., & Lieberman, R. W. (2002) · PubMed · Cited by 30 · see citing works
Foxa1 gene and protein in developing rat eccrine sweat glands
Li, H., Chen, L., Zhang, M., & Zhang, B. (2016) · Journal of Molecular Histology · doi:10.1007/s10735-016-9700-5 · Cited by 22 · see citing works
Self-citationLi, H., Li, X., Zhang, B., Zhang, M., Chen, W., Tang, S., & Fu, X. (2016) · Cell and Tissue Research · doi:10.1007/s00441-016-2357-2 · Cited by 19 · see citing works
Self-citationLi, H., Li, X., Zhang, M., Chen, L., Zhang, B., Tang, S., & Fu, X. (2015) · Journal of Molecular Histology · doi:10.1007/s10735-015-9632-5 · Cited by 18 · see citing works
Self-citationLi, X., Li, H., Zhang, M., Chen, L., & Zhang, B. (2017) · Journal of Molecular Histology · doi:10.1007/s10735-017-9710-y · Cited by 9 · see citing works
Self-citationLi, H., Zhang, M., Chen, L., Zhang, B., & Zhang, C. (2017) · Journal of Molecular Histology · doi:10.1007/s10735-017-9721-8 · Cited by 9 · see citing works
Self-citationLi, H., Chen, L., Zhang, M., & Zhang, B. (2017) · Acta Histochemica · doi:10.1016/j.acthis.2017.07.001 · Cited by 8 · see citing works
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Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids
Du, L., Zhang, L., Zhao, J., Chen, Z., Liu, X., Cao, M., You, L., Zhang, Y., Fu, X., & Li, H. (2022) · Chinese Medical Journal · doi:10.1097/cm9.0000000000001936 · Cited by 6 · see citing works
Self-citationCao, M., Zhao, J., Du, L., Chen, Z., Zhang, L., Liu, X., Cheng, J., Yan, Y., Zhang, C., & Li, H. (2021) · Journal of Molecular Histology · doi:10.1007/s10735-021-10023-z · Cited by 6 · see citing works
Self-citationChen, L., Du, L., Zhang, L., Xie, S., Zhang, X., & Li, H. (2020) · Journal of Molecular Histology · doi:10.1007/s10735-020-09869-6 · Cited by 4 · see citing works
Self-citationKiladze, A. B., & Nk, D. (2019) · Biosystems Diversity · doi:10.15421/011906 · Cited by 1 · see citing works
- Authors
- Zhang, M., Li, H., Xie, S., & Chen, L.
- Affiliations
- Shantou University Medical College (CN)
- Corresponding author
- Li, Haihong
- Venue
- Journal of Molecular Histology
- Published
- 2018-09-20
- Volume
- 49
- Issue
- 6
- Pages
- 567-575
- ISSN
- 1567-2379 (ISSN-L)
- 1567-2387
- OpenAlex
- W2891337241
- Cited by
- 4
- References
- 25
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Molecular Histology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81772102, 81471882)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Dermatology › Cancer and Skin Lesions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- eccrine sweat glands, sweat gland differentiation, sweat ducts, myoepithelial cells, S100P, S100A2, K7, Ca II, GCDFP-15, alpha-smooth muscle actin, cell type markers
- MeSH terms
- Cell Differentiation, Collagen, Drug Combinations, Eccrine Glands, Humans, Laminin, Organ Culture Techniques, Proteoglycans, Regeneration, Time Factors, Biomarkers
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