Cited by 53Cholesterol and Lipid MetabolismAtherosclerosis and Cardiovascular DiseasesLipoproteins and Cardiovascular Health

Trimethylamine-N-Oxide Treatment Induces Changes in the ATP-Binding Cassette Transporter A1 and Scavenger Receptor A1 in Murine Macrophage J774A.1 cells

Mohammadi, Abbas, Najar, Ahmad Gholamhoseynian, Yaghoobi, Mohammad Mehdi, Jahani, Yunes, Vahabzadeh, Zakaria·2016·Inflammation

Abstract

Background Recently, trimethylamine N-oxide was introduced as a risk factor for atherosclerosis in terms of helping foam cell formation and worsening atherosclerosis complications. The present study was performed to investigate whether/how trimethylamine N-oxide is involved in regulation of ATP-binding cassette transporter A1 and scavenger receptor A1 in macrophages at both mRNA and protein levels.Methods Murine macrophage J774A.1 cells were treated with micromolar concentrations of trimethylamine N-oxide and 4-phenylbutyric acid, a chemical chaperon, for different time intervals. Tunicamycin was also used as a control for induction of endoplasmic reticulum stress.Results Similar to tunicamycin, trimethylamine N-oxide increased scavenger receptor A1 in all treatment periods, whereas ATP-binding cassette transporter A1 was only reduced 24 h post-treatment with trimethylamine N-oxide at both mRNA and protein levels. In contrast, 4-phenylbutyric acid failed to induce such changes in either scavenger receptor A1 or ATP-binding cassette transporter A1.Conclusions The results of this study, in agreement with previous studies, confirm the mechanistic role of trimethylamine N-oxide in the upregulation of scavenger receptor A1, which potentially can promote its proatherogenic role. The results also showed downregulation of ATP-binding cassette transporter A1 in trimethylamine N-oxide treated macrophages which may indicate another possible proatherosclerotic mechanism for foam cell formation.

Integrity signals

Registry

Crossref · 10.1007/s10753-015-0261-7

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Mohammadi, A., Najar, A. G., Yaghoobi, M. M., Jahani, Y., & Vahabzadeh, Z.
Affiliations
  • Kerman University of Medical Sciences (IR)
  • Graduate University of Advanced Technology (IR)
Corresponding author
Vahabzadeh, Zakaria
Venue
Inflammation
Published
2015-09-28
Volume
39
Issue
1
Pages
393-404
ISSN
  • 0360-3997 (ISSN-L)
  • 1573-2576
OpenAlex
W2236661062
Cited by
53
References
46
Citations by year
2026: 1 · 2025: 3 · 2024: 7 · 2023: 14 · 2022: 10 · 2021: 4 · 2020: 5 · 2019: 4 · 2018: 3 · 2017: 2
FWCI
1.68
Type
journal article
Language
en
Open access
closed
Locations
  • Inflammation (publishedVersion)
  • PubMed (publishedVersion)
  • Research Information System of Ardabil University of Medical Sciences (Ardabil University of Medical Sciences) (acceptedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
Keywords
trimethylamine N-oxide, ABCA1, macrophages, foam cell formation, atherosclerosis, 4-phenylbutyric acid, chemical chaperones, endoplasmic reticulum stress, tunicamycin, gene expression regulation
MeSH terms
Animals, Cell Line, Foam Cells, Macrophages, Methylamines, Phenylbutyrates, RNA, Messenger, Tunicamycin, Up-Regulation, Atherosclerosis, Scavenger Receptors, Class A, Mice, Endoplasmic Reticulum Stress, ATP Binding Cassette Transporter 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review