A novel method for the fabrication of homogeneous hydroxyapatite/collagen nanocomposite and nanocomposite scaffold with hierarchical porosity
Abstract
Homogeneous nanocomposites composed of hydroxyapatite (HAp) and collagen were synthesized using a novel in situ precipitation method through dual template-driven. The morphological and componential properties of nanocomposites were investigated. The HAp particulates, in sizes of about 50-100 nm, were distributed homogeneously in the organic collagen hydrogel. Highly magnified TEM observation showed that HAp inorganic particles were composed of fine sub-particles (2-5 nm) without regular crystallographic orientation. Based on these homogeneous nanocomposites, a novel HAp/collagen nanocomposite scaffold with hierarchical porosity was prepared by multilevel freeze-drying technique. Compared to other conventional scaffolds for tissue engineering, this novel in situ method endows synthesized composite scaffolds with unique morphology-ultrafine HAp particles dispersed homogenously in collagen at nano level and the foam scaffold with hierarchical pore structures. The mechanical performance increased obviously compared with neat collagen. These results provided an efficient approach toward new biomimetic tissue scaffold for the biomedical applications with enhanced intensity/bioactivity and controlled resorption rates. This novel method, we expect, will lead to a wide application in many other hydrogel systems and may be useful for fabrication of various homogeneous inorganic/organic nanocomposites.
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The amino and carboxyl groups present in collagen served as the site for nucleation and formation of nano-HA.
“The amino and carboxyl groups present in the collagen served as the site for nucleation and formation of nano-HA.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [82]
Shen et al. prepared a nano-HA/collagen scaffold through freeze drying.
“Shen et al [82] prepared the nano-HA/collagen scaffold through freeze drying technique.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [82]
The mechanical properties of the nano-HA/collagen scaffold were improved compared to the collagen scaffold.
“Further, the mechanical properties of the nano-HA/collagen scaffold were found to be improved compared to the collagen scaffold.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [82]
SEM images demonstrated that with an increase in the quantity of cross-linker, the porosity of the scaffold decreased.
“SEM images demonstrated that with the increase in the quantity of the cross-linker, the porosity of the scaffold decreased (figures 6(g)-(j)).”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [82]
The decrease in porosity with increased cross-linker may be due to restriction of nano-HA growth because of compact cross-linking of collagen.
“This phenomenon may be due to the restriction of nano-HA growth because of the compact cross-linking of collagen.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [82]
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Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
- Authors
- Shen, X., Chen, L., Cai, X., Tong, T., Tong, H., & Hu, J.
- Affiliations
- Wuhan University (CN)
- Wuhan Institute of Technology (CN)
- Corresponding author
- Tong, Hua
- Venue
- Journal of Materials Science: Materials in Medicine
- Published
- 2010-12-14
- Volume
- 22
- Issue
- 2
- Pages
- 299-305
- ISSN
- 0957-4530 (ISSN-L)
- 1573-4838
- OpenAlex
- W1971125233
- Cited by
- 61
- References
- 18
- Citations by year
- 2026: 3 · 2024: 2 · 2023: 8 · 2021: 5 · 2020: 8 · 2019: 1 · 2018: 4 · 2017: 8 · 2016: 2 · 2015: 3 · 2014: 6 · 2013: 5 · 2012: 5
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Materials Science Materials in Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- in situ precipitation, collagen gel, hierarchical porosity, tissue engineering scaffolds, mechanical properties, biomimetic scaffolds, nanocomposite morphology
- MeSH terms
- Bone Regeneration, Collagen, Freeze Drying, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Particle Size, Stress, Mechanical, Porosity, Spectroscopy, Fourier Transform Infrared, Durapatite, Bone Substitutes, Tissue Engineering, Biomimetics, Nanotechnology, Microscopy, Electron, Transmission, Nanocomposites
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