Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering
Abstract
To better mimic the mineral component and the microstructure of natural bone, novel nano-hydroxyapatite (NHAP)/polymer composite scaffolds with high porosity and well-controlled pore architectures were prepared using thermally induced phase separation (TIPS) techniques. The morphologies, mechanical properties and protein adsorption capacities of the composite scaffolds were investigated. The high porosity (90% and above) was easily achieved and the pore size was adjusted by varying phase separation parameters. The NHAP particles were dispersed in the pore walls of the scaffolds and bound to the polymer very well. NHAP/polymer scaffolds prepared using pure solvent system had a regular anisotropic but open 3D pore structure similar to plain polymer scaffolds while micro-hydroxyapatite (MHAP)/polymer scaffolds had a random irregular pore structure. The introduction of HAP greatly increased the mechanical properties and improved the protein adsorption capacity. In a dioxane/water mixture solvent system, NHAP-incorporated poly(L-lactic acid) (PLLA) scaffolds developed a fibrous morphology which in turn increased the protein adsorption three fold over non fibrous scaffolds. The results suggest that the newly developed NHAP/polymer composite scaffolds may serve as an excellent 3D substrate for cell attachment and migration in bone tissue engineering.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A nano-HA/PLLA scaffold showed high protein adsorption.
“High protein adsorption was observed [115]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [115]
Cites
Langer, R., & Vacanti, J. P. (1993) · Science · doi:10.1126/science.8493529 · Cited by 9,893 · see citing works
Mechanical properties and the hierarchical structure of bone
Rho, J. Y., Kuhn, L. T., & Zioupos, P. (1998) · Medical Engineering & Physics · doi:10.1016/s1350-4533(98)00007-1 · Cited by 2,596 · see citing works
Properties of osteoconductive biomaterials: calcium phosphates.
Legeros, R. (2002) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-200202000-00009 · Cited by 2,003 · see citing works
Preparation and characterization of poly(l-lactic acid) foams
Mikos, A. G., Thorsen, A. J., Czerwonka, L. A., Bao, Y., Langer, R., Winslow, D. N., & Vacanti, J. P. (1994) · Polymer · doi:10.1016/0032-3861(94)90953-9 · Cited by 1,187 · see citing works
Mooney, D. J., Baldwin, D. F., Suh, N. P., Vacanti, J. P., & Langer, R. (1996) · Biomaterials · doi:10.1016/0142-9612(96)87284-x · Cited by 1,085 · see citing works
Synthetic nano-scale fibrous extracellular matrix
Ma, P. X., & Zhang, R. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199907)46:1 · Cited by 1,055 · see citing works
Self-citationOsteoblast adhesion on nanophase ceramics
Webster, T. J., Siegel, R. W., & Bizios, R. (1999) · Biomaterials · doi:10.1016/s0142-9612(99)00020-4 · Cited by 927 · see citing works
Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds
Ishaug, S. L., Crane, G. M., Miller, M. J., Yasko, A. W., Yaszemski, M. J., & Mikos, A. G. (1997) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199707)36:1 · Cited by 832 · see citing works
A novel method to fabricate bioabsorbable scaffolds
Whang, K., Thomas, C. H., Healy, K. E., & Nuber, G. (1995) · Polymer · doi:10.1016/0032-3861(95)93115-3 · Cited by 639 · see citing works
Atala, A., & Lanza, R. (2002) · Cited by 526 · see citing works
Natural History of Autografts and Allografts
Goldberg, V. M., & Stevenson, S. (1987) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-198712000-00003 · Cited by 436 · see citing works
Natural History of Autografts and Allografts
Goldberg, V. (1992) · Springer eBooks · doi:10.1007/978-1-4471-1934-0_2 · Cited by 411 · see citing works
Hydroxyapatite fiber reinforced poly(α-hydroxy ester) foams for bone regeneration
Thomson, R. C., Yaszemski, M. J., Powers, J. M., & Mikos, A. G. (1998) · Biomaterials · doi:10.1016/s0142-9612(98)00097-0 · Cited by 408 · see citing works
Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture
Du, C., Cui, F. Z., Zhu, X. D., & de Groot, K. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19990315)44:4 · Cited by 365 · see citing works
Microtubular architecture of biodegradable polymer scaffolds
Ma, P. X., & Zhang, R. (2001) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(20010915)56:4 · Cited by 343 · see citing works
Self-citationFeasibility study of a natural crosslinking reagent for biological tissue fixation
Sung, H., Huang, R., Huang, L. L. H., Tsai, C.-C., & Chiu, C.-T. (1998) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19981215)42:4 · Cited by 307 · see citing works
Biodegradation of and tissue reaction to 50:50 poly(DL‐lactide‐co‐glycolide) microcapsules
Visscher, G. E., Robison, R. L., Maulding, H. V., et al. (1985) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820190315 · Cited by 222 · see citing works
Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering
Li, S. H., de Wijn, J. R., Layrolle, P., et al. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10163 · Cited by 213 · see citing works
Synthetic Bone Graft Substitutes
Costantino, P. D., & Friedman, C. D. (1994) · Otolaryngologic Clinics of North America · doi:10.1016/s0030-6665(20)30622-8 · Cited by 190 · see citing works
Flautre, B., Descamps, M., Delecourt, C., Blary, M. C., & Hardouin, P. (2001) · Journal of Materials Science Materials in Medicine · doi:10.1023/a:1011256107282 · Cited by 180 · see citing works
The sintered microsphere matrix for bone tissue engineering: In vitro osteoconductivity studies
Borden, M. D., ATTAWIA, M. A., & Laurencin, C. T. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10201 · Cited by 154 · see citing works
Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., & Aguado, E. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199910)47:1 · Cited by 148 · see citing works
Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells
Ramires, P. A., Mirenghi, L., Romano, A. R., Palumbo, F., & Nicolardi, G. (2000) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(20000905)51:3 · Cited by 127 · see citing works
Immunological Responses to Bone
BONFIGLID, M., & Jeter, W. S. (1972) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-197209000-00005 · Cited by 102 · see citing works
Ceramics in Orthopedic Surgery
Cooke, F. W. (1992) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-199203000-00017 · Cited by 99 · see citing works
Born Again Bone: Tissue Engineering for Bone Repair
Braddock, M., Houston, P., Campbell, C. J., et al. (2001) · Physiology · doi:10.1152/physiologyonline.2001.16.5.208 · Cited by 94 · see citing works
Modulation of corneal epithelial stratification by polymer surface topography
Dalton, B. A., Evans, M. D. M., McFarland, G. A., & Steele, J. G. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19990615)45:4 · Cited by 72 · see citing works
Labow, R. S., Meek, E., & Santerre, J. P. (2000) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(200102)54:2 · Cited by 69 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047 · see citing works
Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering
Swetha, M., Sahithi, K., Moorthi, A., Srinivasan, N., Ramasamy, K., & Selvamurugan, N. (2010) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2010.03.015 · Cited by 548 · see citing works
High-strength silk protein scaffolds for bone repair
Mandal, B. B., Grinberg, A., Seok Gil, E., Panilaitis, B., & Kaplan, D. L. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1119474109 · Cited by 401 · see citing works
Significance of synthetic nanostructures in dictating cellular response
Yim, E. K. F., & Leong, K. W. (2005) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2004.11.008 · Cited by 281 · see citing works
Akbarzadeh, R., & Yousefi, A. (2014) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.33101 · Cited by 208 · see citing works
Cunniffe, G. M., Dickson, G. R., Partap, S., Stanton, K. T., & O’Brien, F. J. (2010) · Journal of Materials Science: Materials in Medicine · doi:10.1007/s10856-009-3964-1 · Cited by 199 · see citing works
Cunniffe, G. M., Curtin, C. M., Thompson, E. M., Dickson, G. R., & O’Brien, F. J. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b06596 · Cited by 95 · see citing works
Wang, T., Yang, X., Qi, X., & Jiang, C. (2015) · Journal of Translational Medicine · doi:10.1186/s12967-015-0499-8 · Cited by 83 · see citing works
Shen, X., Chen, L., Cai, X., Tong, T., Tong, H., & Hu, J. (2011) · Journal of Materials Science: Materials in Medicine · doi:10.1007/s10856-010-4199-x · Cited by 61 · see citing works
Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
G, R., S, B., Venkatesan, B., & Vellaichamy, E. (2017) · Materials Science and Engineering: C · doi:10.1016/j.msec.2016.12.133 · Cited by 56 · see citing works
Wang, H., Zuo, Y., Zou, Q., Cheng, L., Huang, D., Wang, L., & Li, Y. (2009) · Journal of Polymer Science Part A: Polymer Chemistry · doi:10.1002/pola.23171 · Cited by 48 · see citing works
Semyari, H., Salehi, M., Taleghani, F., Ehterami, A., Bastami, F., Jalayer, T., Semyari, H., Hamed Nabavi, M., & Semyari, H. (2018) · Journal of Biomaterials Applications · doi:10.1177/0885328218805229 · Cited by 45 · see citing works
Enhancing bioactivity of hydroxyapatite scaffolds using fibrous type I collagen
Nitti, P., Kunjalukkal Padmanabhan, S., Cortazzi, S., Stanca, E., Siculella, L., Licciulli, A., & Demitri, C. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.631177 · Cited by 35 · see citing works
Nanobiocomposite based on natural polyelectrolytes for bone regeneration
Belluzo, M. S., Medina, L. F., Molinuevo, M. S., Cortizo, M. S., & Cortizo, A. M. (2020) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36917 · Cited by 8 · see citing works
- Authors
- Wei, G., & Ma, P. X.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Ma, Peter X.
- ORCID
- Ma, Peter X.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2004-08
- Volume
- 25
- Issue
- 19
- Pages
- 4749-4757
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2021790237
- Cited by
- 1,271
- References
- 28
- Citations by year
- 2026: 21 · 2025: 24 · 2024: 29 · 2023: 42 · 2022: 56 · 2021: 73 · 2020: 61 · 2019: 83 · 2018: 71 · 2017: 83 · 2016: 96 · 2015: 62 · 2014: 75 · 2013: 76 · 2012: 83
- FWCI
- 24.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- hydroxyapatite nanoparticles, bone tissue engineering, thermally induced phase separation, porosity control, pore structure, mechanical properties, protein adsorption, poly(L-lactic acid), fibrous morphology
- MeSH terms
- Adsorption, Animals, Blood Proteins, Cattle, Elasticity, Manufactured Materials, Materials Testing, Membranes, Artificial, Molecular Conformation, Particle Size, Polyesters, Polymers, Surface Properties, Porosity, Durapatite, Bone Substitutes, Cell Culture Techniques, Compressive Strength, Lactic Acid, Tissue Engineering, Nanotubes, Macromolecular Substances
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review