Alteration of mitochondrial oxidative capacity during porcine preadipocyte differentiation and in response to leptin.
Abstract
Mitochondrial apparatus is a fundamental aspect in cell, serving for amino acid biosynthesis, fatty acid oxidation (FAO), and ATP production. In this article, we investigated the change of mitochondrial oxidative capacity during porcine adipocyte differentiation and in response to leptin. Rhodamine 123 staining analysis showed about 2-fold increase of mitochondrial membrane electric potential in differentiated adipocyte in comparison with preadipocyte. The mRNA expression of Cytochromes c (Cyt c), carnitine palmitoyltransferase 1 (CPT1), and malate dehydrogenases (MDH) increased markedly (P < 0.05), but that of UCP2 decreased (P < 0.05). Moreover PGC-1alpha and UCP3 was very low and showed no changes during the adipocyte differentiation. The protein expression of Cyt c and the enzyme activity of Cytochrome c oxidase (COX) increased with preadipocyte differentiation, but cellular ATP level decreased. Furthermore, at the level of 10 and 100 ng/ml leptin not only selectively increased the gene expression of PGC-1alpha, CPT1, Cyt c, UCP2, and UCP3 (P < 0.05), but also enhanced COX enzyme activity which related to mitochondrial FAO. There is no change of Mitochondrial membrane electric potential and ATP level in cell treated by leptin. These results suggested Mitochondrial is not only critical in FAO, but also play an important role in adipogenesis.
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Mitochondrial density increases dramatically, and mitochondrial remodeling occurs during white adipocyte differentiation, suggesting that mitochondrial functions are required to support the multiple biological roles of mature white adipocytes.
“However, mitochondrial density increases dramatically, and mitochondrial remodeling occurs during white adipocyte differentiation (156 -158), suggesting that mitochondrial functions are required to support the multiple biological roles of mature white adipocytes.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [156]
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The role of mitochondria in the pathogenesis of type 2 diabetes
Patti, M.-E., & Corvera, S. (2010) · Endocrine Reviews · doi:10.1210/er.2009-0027 · Cited by 551 · see citing works
- Authors
- Luo, G.-F., Yu, T.-Y., Wen, X.-H., Li, Y., & Yang, G.-S.
- Affiliations
- Northwest A&F University (CN)
- Corresponding author
- Yang, Gong-She
- ORCID
- Li, Ying
- Venue
- Molecular and Cellular Biochemistry
- Published
- 2007-10-02
- Volume
- 307
- Issue
- 1-2
- Pages
- 83-91
- ISSN
- 0300-8177 (ISSN-L)
- 1573-4919
- OpenAlex
- W2107727447
- Cited by
- 28
- References
- 30
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2015: 4 · 2013: 3 · 2012: 1
- FWCI
- 2.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and Cellular Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- mitochondrial oxidative capacity, adipogenesis, preadipocytes, leptin, porcine adipocytes, mitochondrial membrane potential, cytochrome c, carnitine palmitoyltransferase 1, malate dehydrogenase, uncoupling proteins, PGC-1α, cytochrome c oxidase, fatty acid oxidation, ATP production
- MeSH terms
- Animals, Cell Differentiation, Male, Mitochondria, Oxidation-Reduction, Swine, Transcription Factors, Gene Expression, Adipocytes, Cell Respiration, Leptin
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