Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells
Abstract
The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.
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IL-15 antagonizes the detrimental actions of TNF-α, considering its antioxidant properties and its ability to stimulate adiponectin release.
“Meanwhile, IL-15 was noted to antagonize the detrimental actions of TNF-α, considering its antioxidant properties [26,87] and its ability to stimulate adiponectin release [86] (6).”
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- Authors
- Li, F., Li, Y., Tang, Y., Lin, B., Kong, X., Oladele, O. A., & Yin, Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Yin, Yulong
- Venue
- Molecular Biology Reports
- Published
- 2014-08-08
- Volume
- 41
- Issue
- 11
- Pages
- 7715-7722
- ISSN
- 0301-4851 (ISSN-L)
- 1573-4978
- OpenAlex
- W1987769700
- Cited by
- 48
- References
- 39
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 3
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Biology Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- IL-15, myokines, oxidative stress, hydrogen peroxide, reactive oxygen species, skeletal muscle cells, C2C12 myoblasts, mitochondrial network, UCP3, interleukin-6, p21, APE1, Akt signaling, cell viability, creatine kinase, lactate dehydrogenase
- MeSH terms
- Analysis of Variance, Animals, Cell Line, Creatine Kinase, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Hydrogen Peroxide, L-Lactate Dehydrogenase, Blotting, Western, Interleukin-6, Reactive Oxygen Species, DNA Primers, Oxidative Stress, Muscle, Skeletal, Interleukin-15, Myoblasts, Mice, Real-Time Polymerase Chain Reaction
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