Cited by 48Exercise and Physiological ResponsesMuscle Physiology and DisordersRedox biology and oxidative stress

Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells

Li, Fengna, Li, Yinghui, Tang, Yulong, Lin, Binbin, Kong, Xiangfeng, Oladele, Oso Abimbola, Yin, Yulong·2014·Molecular Biology Reports

Abstract

The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.

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Crossref · 10.1007/s11033-014-3665-9

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Authors
Li, F., Li, Y., Tang, Y., Lin, B., Kong, X., Oladele, O. A., & Yin, Y.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Yin, Yulong
Venue
Molecular Biology Reports
Published
2014-08-08
Volume
41
Issue
11
Pages
7715-7722
ISSN
  • 0301-4851 (ISSN-L)
  • 1573-4978
OpenAlex
W1987769700
Cited by
48
References
39
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 3
FWCI
3.88
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Biology Reports (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
IL-15, myokines, oxidative stress, hydrogen peroxide, reactive oxygen species, skeletal muscle cells, C2C12 myoblasts, mitochondrial network, UCP3, interleukin-6, p21, APE1, Akt signaling, cell viability, creatine kinase, lactate dehydrogenase
MeSH terms
Analysis of Variance, Animals, Cell Line, Creatine Kinase, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Hydrogen Peroxide, L-Lactate Dehydrogenase, Blotting, Western, Interleukin-6, Reactive Oxygen Species, DNA Primers, Oxidative Stress, Muscle, Skeletal, Interleukin-15, Myoblasts, Mice, Real-Time Polymerase Chain Reaction

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