Involvement of Endoplasmic Reticulum Stress on the Cell Death Induced by 6-Hydroxydopamine in Human Neuroblastoma SH-SY5Y Cells
Abstract
Endoplasmic reticulum (ER) dysfunction is known to activate the unfolded protein response, which is characterized by the activation of two divergent processes, i.e., suppression of the initiation process in global protein synthesis and expression of glucose-regulated protein 78 (Bip/Grp78) and the C/EBP homologous transcription factor CHOP/Gadd153. In this study, we examined the expression of CHOP/Gadd153 and Bip/Grp78 in human neuroblastoma SH-SY5Y cells treated with 6-hydroxydopamine (6-OHDA), which is used to prepare animal models of Parkinson's disease. 6-OHDA treatment induced cell death, in a concentration-dependent manner, which was inhibited by co-treatment with an antioxidant N-acetylcysteine. 6-OHDA was also effective in decreasing proteasome activity and in increasing the levels of high molecular ubiquitin-conjugated proteins. Furthermore, 6-OHDA induced a marked increase in the expression of both CHOP/Gadd153 and Bip/Grp78. This increase was prevented by N-acetylcysteine. Taken together, our data indicate that ER dysfunction is at least in part involved in the mechanisms underlying cell death induced by 6-OHDA in SH-SY5Y cells.
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Oligodendrocytes have equal amounts of both H and L subunits, microglia express L-rich ferritin, and neurons have H-subunit abundant ferritin.
“Oligodendrocytes have equal amounts of both H and L subunits, whereas microglia express L-rich ferritin, and neurons have H-subunit abundant ferritin (206, 333).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [333]
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- Authors
- Yamamuro, A., Yoshioka, Y., Ogita, K., & Maeda, S.
- Affiliations
- Setsunan University (JP)
- Corresponding author
- Yamamuro, Akiko
- ORCID
- Yoshioka, Yasuhiro
- Venue
- Neurochemical Research
- Published
- 2006-05
- Volume
- 31
- Issue
- 5
- Pages
- 657-664
- ISSN
- 0364-3190 (ISSN-L)
- 1573-6903
- OpenAlex
- W2036377920
- Cited by
- 70
- References
- 43
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 5 · 2018: 1 · 2017: 4 · 2015: 1 · 2014: 4 · 2012: 4
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Neurochemical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- endoplasmic reticulum stress, 6-hydroxydopamine, neurotoxicity, cell death, SH-SY5Y cells, neuroblastoma, unfolded protein response, CHOP/GADD153, GRP78, N-acetylcysteine, proteasome activity, ubiquitination, Parkinson's disease model, antioxidants
- MeSH terms
- Endoplasmic Reticulum Chaperone BiP, Acetylcysteine, Animals, Endoplasmic Reticulum, Heat-Shock Proteins, Humans, Neoplasm Proteins, Neuroblastoma, Parkinson Disease, Tunicamycin, Free Radical Scavengers, Oxidopamine, Cell Death, Adrenergic Agents, Molecular Chaperones, Ubiquitin, Cell Line, Tumor, Proteasome Endopeptidase Complex, Activating Transcription Factor 6, Transcription Factor CHOP, Proteasome Inhibitors
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