The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The PGRMC1 Protein Level Correlates with the Binding Activity of a Sigma-2 Fluorescent Probe (SW120) in Rat Brain Cells
Abstract
Purpose We examined the progesterone receptor membrane component 1 (PGRMC1) protein expression in rat brain cells as the prerequisite step to understand the biology of PGRMC1 in the central nervous system (CNS). We also performed correlation studies between the PGRMC1 protein level and the binding activity of a sigma-2 fluorescent probe, SW120, in order to explore the possibility of using sigma-2 radiotracer of positron emission tomography (PET) to noninvasively image the CNS. Procedures Embryonic primary neurons, astrocytes, oligodendrocytes, and microglia cells were cultured. Immunocytochemistry, Western blot, and SW120 staining were performed in these cells. Results The protein expression of PGRMC1 determined by immunocytochemistry and SW120 staining is prominent in neurons and relatively low in astrocytes, oligodendrocytes, and microglia cells. The PGRMC1 expression level correlates with the binding activity of SW120 in rat brain cells. Conclusions The sigma-2 receptor PET radiotracer can be potentially used to noninvasively image neuron/synapse densities in the CNS.
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TMEM97 and PGRMC1 are expressed much higher in neurons than in glial cells.
“previous studies have shown that they are expressed much higher in neurons than in glial cells [6, 7, 47].”
From The Sigma-2 Receptor/TMEM97, PGRMC1, and LDL Receptor Complex Are Responsible for the Cellular Uptake of Aβ42 and Its Protein Aggregates (2020) · cites this paper as [47]
TMEM97 and PGRMC1 specific ligands bind their targets more prominently, and with much higher affinity, in neurons than in glial cells.
“Moreover, TMEM97 and PGRMC1 specific ligands bind their targets more prominently, and with much higher affinity, in neurons than in glial cells [6, 7, 47].”
From The Sigma-2 Receptor/TMEM97, PGRMC1, and LDL Receptor Complex Are Responsible for the Cellular Uptake of Aβ42 and Its Protein Aggregates (2020) · cites this paper as [47]
S2R activity was initially localized to a ~20 kDa membrane protein and first thought to be progesterone receptor membrane component 1 (PGRMC1).
“S2R activity was initially localized to a ~20 kDa membrane protein [51,52] and first thought to be progesterone receptor membrane component 1 (PGRMC1), based on mass spectrophotometry identification following an immunoaffinity pull down with an irreversible S2R ligand binder WC-11, physiochemical properties, and pharmacological evidence [53,54].”
From Sigma-2 Receptors—From Basic Biology to Therapeutic Target: A Focus on Age-Related Degenerative Diseases (2023) · cites this paper as [53]
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Cited by
Sorted by citations
Riad, A., Lengyel-Zhand, Z., Zeng, C., Weng, C.-C., Lee, V. M.-Y., Trojanowski, J. Q., & Mach, R. H. (2020) · Molecular Neurobiology · doi:10.1007/s12035-020-01988-1 · Cited by 81
Self-citationLizama, B. N., Kahle, J., Catalano, S. M., Caggiano, A. O., Grundman, M., & Hamby, M. E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076251 · Cited by 54
Metadata
- Authors
- Zeng, C., Garg, N., & Mach, R. H.
- Venue
- Molecular Imaging and Biology
- Published
- 2015-09-03
- Volume
- 18
- Issue
- 2
- Pages
- 172-179
- ISSN
- 1536-1632
- 1860-2002
- Cited by
- 23
- References
- 1
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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