Cited by 135Gut microbiota and healthPesticide Exposure and ToxicityImmunotoxicology and immune responses

Impact of chronic exposure to low doses of chlorpyrifos on the intestinal microbiota in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in the rat

Joly, Claire, Gay-Quéheillard, Jérôme, Léké, André, Chardon, Karen, Delanaud, Stéphane, Bach, Véronique, Khorsi-Cauet, Hafida·2013·Environmental Science and Pollution Research

Abstract

The impact of the insecticide chlorpyrifos (CPF) on the mammalian digestive system has been poorly described. The present study aimed at evaluating the effect of chronic, low-dose exposure to CPF on the composition of the gut microbiota in a Simulator of the Human Intestinal Microbial Ecosystem: the SHIME and in rats. The SHIME comprises six reactor vessels (stomach to colon). The colonic segments were inoculated with feces from healthy humans. Then, the simulator was exposed to a daily dose of 1 mg of CPF for 30 days. The changes over time in the populations of bacteria were examined at different time points: prior to pesticide exposure (as a control) and after exposure. In parallel, pregnant rats were gavaged daily with 1 mg/kg of CPF (or vehicle) until the pups were weaned. Next, the rats were gavaged with same dose of CPF until 60 days of age (adulthood). Then, samples of different parts of the digestive tract were collected under sterile conditions for microbiological assessment. Chronic, low-dose exposure to CPF in the SHIME and in the rat was found to induce dysbiosis in the microbial community with, in particular, proliferation of subpopulations of some strains and a decrease in the numbers of others bacteria. In compliance with European guidelines, the use of the SHIME in vitro tool would help to (1) elucidate the final health effect of toxic agents and (2) minimize (though not fully replace) animal testing. Indeed, certain parameters would still have to be studied further in vivo.

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Crossref · 10.1007/s11356-012-1283-4

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  • Chronic exposure to low doses of the insecticide chlorpyrifos changed the composition of the gut microbiota in both a simulated human intestinal microbiota preparation and rats, reducing the abundance of Lactobacillus spp. and Bifidobacterium spp.

    “Chronic exposure to low doses of the insecticide, Chlorpyrifos, changed the composition of the gut microbiota in both a simulated human intestinal microbiota preparation and rats, reducing the abundance of Lactobacillus spp.and Bifidobacterium spp, bacteria presumed to be beneficial (Joly et al., 2013).”

    From Gut reactions: Breaking down xenobiotic–microbiome interactions (2019) · cites this paper as Joly et al., 2013

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Authors
Joly, C., Gay-Quéheillard, J., Léké, A., Chardon, K., Delanaud, S., Bach, V., & Khorsi-Cauet, H.
Affiliations
Institut national de l'environnement industriel et des risques (FR)
Venue
Environmental Science and Pollution Research
Published
2012-11-08
Volume
20
Issue
5
Pages
2726-2734
ISSN
  • 0944-1344 (ISSN-L)
  • 1614-7499
OpenAlex
W2095549625
Cited by
135
References
47
Citations by year
2026: 4 · 2025: 13 · 2024: 13 · 2023: 5 · 2022: 10 · 2021: 15 · 2020: 10 · 2019: 16 · 2018: 15 · 2017: 12 · 2016: 7 · 2015: 8 · 2014: 6 · 2013: 1
FWCI
1.93
Type
journal article
Language
en
Open access
closed
Locations
  • Environmental Science and Pollution Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
Conseil Régional de Picardie
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Pesticide Exposure and Toxicity
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
Keywords
chlorpyrifos, gut microbiota, pesticide exposure, chronic low-dose exposure, dysbiosis, SHIME, in vitro gut model, rat model, intestinal bacteria, alternatives to animal testing
MeSH terms
Animals, Dose-Response Relationship, Drug, Chlorpyrifos, Environmental Exposure, Environmental Monitoring, Environmental Pollutants, Female, Humans, Insecticides, Intestines, Male, Models, Biological, Time Factors, Rats, Metagenome

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