Cited by 33Antioxidant Activity and Oxidative StressAlcohol Consumption and Health EffectsRedox biology and oxidative stress

Acetaldehyde-Induced Oxidative Modifications and Morphological Changes in Isolated Human Erythrocytes: An in Vitro Study

Waris, Sana, Patel, Ayyub, Ali, Asif, Mahmood, Riaz·2020·Environmental Science and Pollution Research

Abstract

Acetaldehyde is a toxic, mutagenic and carcinogenic metabolite of alcohol which can bind to proteins, DNA and several other cellular macromolecules. Chronic alcohol consumption increases intracellular acetaldehyde levels which enhances the generation of reactive oxygen and nitrogen species (ROS and RNS). In this study, we have examined the effect of acetaldehyde on human erythrocytes under in vitro conditions. Treatment of human erythrocytes with different concentrations of acetaldehyde (0.05-2 mM) for 24 h at 37 °C increased intracellular generation of ROS and RNS. It also increased oxidation of proteins and lipids but decreased glutathione, total sulphhydryl and free amino group content. Methemoglobin level was increased accompanied by a decrease in methemoglobin reductase activity. Acetaldehyde impaired the antioxidant defence system and lowered the total antioxidant capacity of the cell. It decreased the activity of metabolic and membrane-bound enzymes and altered erythrocyte morphology. Our results show that acetaldehyde enhances the generation of ROS and RNS that results in oxidative modification of cellular components. This will lower the oxygen transporting ability of blood and shorten erythrocyte lifespan (red cell senescence).

Integrity signals

Registry

Crossref · 10.1007/s11356-020-08044-4

Cites

93 of 93 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Waris, S., Patel, A., Ali, A., & Mahmood, R.
Affiliations
  • Aligarh Muslim University (IN)
  • King Khalid University (SA)
Corresponding author
Mahmood, Riaz
Venue
Environmental Science and Pollution Research
Published
2020-03-02
Volume
27
Issue
14
Pages
16268-16281
ISSN
  • 0944-1344 (ISSN-L)
  • 1614-7499
OpenAlex
W3010490567
Cited by
33
References
93
Citations by year
2026: 2 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 10 · 2021: 6 · 2020: 4
FWCI
3.39
Type
journal article
Language
en
Open access
closed
Locations
  • Environmental Science and Pollution Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
acetaldehyde, human erythrocytes, oxidative stress, reactive oxygen species, reactive nitrogen species, protein oxidation, lipid peroxidation, glutathione depletion, methemoglobin, methemoglobin reductase, antioxidant capacity, erythrocyte morphology, alcohol consumption
MeSH terms
Acetaldehyde, Erythrocytes, Glutathione, Humans, Oxidation-Reduction, Reactive Oxygen Species, Oxidative Stress

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review