CoChat
Sign Up
Cited by 23Spaceflight effects on biologyGastrointestinal motility and disordersBarrier Structure and Function Studies

Effects of simulated weightlessness on tight junction protein occludin and Zonula Occluden-1 expression levels in the intestinal mucosa of rats

Ying, Chen, Chunmin, Yang, Qingsen, Liu, Mingzhou, Guo, Yunsheng, Yang, Gaoping, Mao, Ping, Wang·2011·Journal of Huazhong University of Science and Technology [Medical Sciences]
DOI 10.1007/s11596-011-0145-5
Open this paper in CoChat — free account

Abstract

This study investigated the tight junction (TJ) protein expression of the intestinal mucosa in a rat tail-suspension model under simulated weightlessness. Twenty-four Wistar rats were randomly divided into three groups: CON group (n=8), control; SUS-14 d group (n=8), tail-suspension for 14 days; SUS-21 d group (n=8), tail-suspension for 21 days. Occludin and Zonula Occluden-1 (ZO-1) expression levels were determined by immunohistochemical analysis and mRNA fluorescent quantitative PCR. Plasma levels of diamine oxidase (DAO) and d-lactate were determined using enzymatic spectrophotometry. Immunohistochemical results for occludin and ZO-1 showed disruption of the TJs in the intestinal mucosa in SUS-14 d and SUS-21 d groups. The expression levels of occludin and ZO-1 in SUS-21 d group were lower than those in SUS-14 d group, and significantly lower than those in CON group (Occldin: 0.86±0.02 vs 1.01±0.03 vs 1.63±0.03 and ZO-1: 0.82±0.01 vs 1.00±0.02 vs 1.55±0.01, P<0.01). Moreover, the levels of plasma DAO and d-lactate in SUS-21 d group were higher than those in SUS-14 d group, and significantly higher than those in CON group (DAO: 27.58±0.49 vs 20.74±0.49 vs 12.94±0.21 and d-lactate: 37.86±0.74 vs 28.26±1.01 vs 17.76±0.91, P<0.01). There were significant negative correlations between occludin or ZO-1 expression levels and DAO (r (2)=0.9014, r (2)=0.9355, P<0.01) or d-lactate levels (r (2)=0.8989, r (2)=0.9331, P<0.01). Occludin and Zo-1 were reduced in intestinal mucosa both in mRNA and protein levels in the rat tail-suspension model. The significant negative correlations between expression levels of TJs and plasma levels of DAO or d-lactate support the hypothesis that intestinal permeability is increased due to a decrease in TJ protein expression during tail-suspension from 14 days to 21 days.

Integrity signals

Registry

Crossref · 10.1007/s11596-011-0145-5

Cites

20 of 20 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Nonsurgical causes of intestinal hyperpermeability also increase absorption of D-lactate from the intestinal lumen.

    “Nonsurgical causes of intestinal hyperpermeability also increase absorption of D-lactate from the intestinal lumen.63,64”

    From The Gut Microbiome and the Brain (2014) · cites this paper as [64]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Ying, C., Chunmin, Y., Qingsen, L., Mingzhou, G., Yunsheng, Y., Gaoping, M., & Ping, W.
Affiliations
  • Air Force General Hospital PLA (CN)
  • Chinese PLA General Hospital (CN)
Corresponding author
Qingsen, Liu
ORCID
Yunsheng, Yang
Venue
Journal of Huazhong University of Science and Technology [Medical Sciences]
Publisher
Springer Science and Business Media LLC
Published
2011-02
Volume
31
Issue
1
Pages
26-32
ISSN
  • 1672-0733 (ISSN-L)
  • 1993-1352
DOI
10.1007/s11596-011-0145-5
OpenAlex
W64580047
Cited by
23
References
20
Citations by year
2026: 5 · 2024: 3 · 2023: 2 · 2021: 1 · 2020: 3 · 2019: 1 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2
FWCI
0.65
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Huazhong University of Science and Technology [Medical Sciences] (publishedVersion)
  • PubMed (publishedVersion)
Funders
Academy of Medical Sciences
Topics
  • Health Sciences › Medicine › Physiology › Spaceflight effects on biology
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
tight junction proteins, occludin, zonula occludens-1, simulated microgravity, tail suspension test, intestinal mucosa, intestinal permeability, diamine oxidase, D-lactate, tight junction disruption, rats, mRNA expression, protein expression
MeSH terms
Animals, Intestinal Mucosa, Male, RNA, Messenger, Rats, Wistar, Weightlessness Simulation, Hindlimb Suspension, Rats, Occludin, Zonula Occludens-1 Protein

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review