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Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging

Open access
Ge, Yuqing, Zhang, Yu, He, Shiying, Nie, Fang, Teng, Gaojun, Gu, Ning·2009·Nanoscale Research Letters

Abstract

Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 10 4 by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.

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Metadata

Authors
Ge, Y., Zhang, Y., He, S., Nie, F., Teng, G., & Gu, N.
Venue
Nanoscale Research Letters
Published
2009-01-16
Volume
4
Issue
4
Pages
287-295
ISSN
1556-276X (ISSN-L)
OpenAlex
W2159838744
Cited by
233
References
1
FWCI
9.51
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging. https://app.cochat.ai/papers/10.1007/s11671-008-9239-9
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Ge, Y., Zhang, Y., He, S., Nie, F., Teng, G., & Gu, N. (2009). Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging. Nanoscale Research Letters. https://doi.org/10.1007/s11671-008-9239-9

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