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Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging
Open accessAbstract
Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 10 4 by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.
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Yin Win, K., & Feng, S.-S. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.07.050 · Cited by 1,452
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Metadata
- Authors
- Ge, Y., Zhang, Y., He, S., Nie, F., Teng, G., & Gu, N.
- Venue
- Nanoscale Research Letters
- Published
- 2009-01-16
- Volume
- 4
- Issue
- 4
- Pages
- 287-295
- ISSN
- 1556-276X (ISSN-L)
- OpenAlex
- W2159838744
- Cited by
- 233
- References
- 1
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ge, Y., Zhang, Y., He, S., Nie, F., Teng, G., & Gu, N. (2009). Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging. Nanoscale Research Letters. https://doi.org/10.1007/s11671-008-9239-9
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