The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed ( Cannabis sativa L.) Protein Hydrolysates
Abstract
The aim of this study was to determine the antihypertensive effects of enzymatic hemp seed protein hydrolysate (HPH) and its peptide fractions. Hemp seed protein isolate was digested by the sequential action of pepsin and pancreatin to mimic gastrointestinal digestion in human beings. The resultant HPH was separated by membrane ultrafiltration into peptide fractions with different sizes (<1 and 1–3 kDa). The HPH led to significantly higher ( P < 0.05) in vitro inhibition of the activities of angiotensin I‐converting enzyme (ACE) and renin, the two main enzymes involved in abnormal blood pressure elevation (hypertension). Kinetic studies showed that HPH and peptide fractions inhibited renin and ACE activities in a mixed‐type pattern, indicating binding to areas other than the active site. Oral administration of HPH (200 mg/kg body weight) to spontaneously hypertensive rats led to significant reductions ( P < 0.05) in systolic blood pressure (SBP) that reached a maximum of −30 mmHg after 8 h. In contrast, the hypotensive effects of peptide fractions (<1 and 1–3 kDa) had a maximum value of about −15 mmHg after 6–8 h post oral administration. The results suggest a synergistic antihypertensive effect of the peptides present within HPH; this effect was reduced significantly ( P < 0.05) upon separation into peptide fractions.
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“These values are in the range of previous results obtained on hemp seed protein hydrolysates.”
From Comparing the Effectiveness of Three Different Biorefinery Processes at Recovering Bioactive Products from Hemp (Cannabis sativa L.) Byproduct (2020) · cites this paper as Girgih et al. 2011
In the citing paper
“In particular, a pepsin-pancreatin digest of hemp seed protein isolate produced hydrolysates with ACE-inhibitory activity and IC50 value of 670 mg/L (Girgih et al. 2011).”
From Comparing the Effectiveness of Three Different Biorefinery Processes at Recovering Bioactive Products from Hemp (Cannabis sativa L.) Byproduct (2020) · cites this paper as Girgih et al. 2011
Citations
Cites
Physicochemical and Functional Properties of Hemp ( Cannabis sativa L.) Protein Isolate
Tang, C.-H., Ten, Z., Wang, X.-S., & Yang, X.-Q. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0619176 · Cited by 405
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Cited by
Sorted by citations
Food Protein‐Derived Bioactive Peptides: Production, Processing, and Potential Health Benefits
Udenigwe, C. C., & Aluko, R. E. (2011) · Journal of Food Science · doi:10.1111/j.1750-3841.2011.02455.x · Cited by 1,069
Self-citationAntihypertensive Peptides from Food Proteins
Aluko, R. E. (2015) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-022814-015520 · Cited by 404
Self-citationGirgih, A. T., He, R., Malomo, S. A., Offengenden, M., Wu, J., & Aluko, R. E. (2013) · Journal of Functional Foods · doi:10.1016/j.jff.2013.11.005 · Cited by 308
Self-citationProcessing, Nutrition, and Functionality of Hempseed Protein: A Review
Wang, Q., & Xiong, Y. L. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12450 · Cited by 229
Antioxidant properties of Australian canola meal protein hydrolysates.
Alashi, A., Blanchard, C., Mailer, R., Agboola, S., Mawson, A. J., He, R., Girgih, A., & Aluko, R. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2013.09.081 · Cited by 183
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Metadata
- Authors
- Girgih, A. T., Udenigwe, C. C., Li, H., Adebiyi, A. P., & Aluko, R. E.
- Affiliations
- University of Manitoba (CA)
- Corresponding author
- Aluko, Rotimi E.
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 2011-05-10
- Volume
- 88
- Issue
- 11
- Pages
- 1767-1774
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W2093049132
- Cited by
- 174
- References
- 0
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 12 · 2023: 7 · 2022: 12 · 2021: 15 · 2020: 15 · 2019: 12 · 2018: 11 · 2017: 17 · 2016: 17 · 2015: 11 · 2014: 9 · 2013: 16 · 2012: 4
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Oil Chemists Society (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Research Executive Agency
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
- Keywords
- Cannabis sativa, antihypertensive peptides, angiotensin-converting enzyme inhibition, renin inhibition, gastrointestinal digestion, spontaneously hypertensive rats, blood pressure reduction, synergistic effect
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