Cited by 174Protein Hydrolysis and Bioactive PeptidesRenin-Angiotensin System StudiesPeptidase Inhibition and Analysis

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Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed ( Cannabis sativa L.) Protein Hydrolysates

Girgih, Abraham T., Udenigwe, Chibuike C., Li, Huan, Adebiyi, Abayomi P., Aluko, Rotimi E.·2011·Journal of the American Oil Chemists' Society

DOI 10.1007/s11746-011-1841-9

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Abstract

The aim of this study was to determine the antihypertensive effects of enzymatic hemp seed protein hydrolysate (HPH) and its peptide fractions. Hemp seed protein isolate was digested by the sequential action of pepsin and pancreatin to mimic gastrointestinal digestion in human beings. The resultant HPH was separated by membrane ultrafiltration into peptide fractions with different sizes (<1 and 1–3 kDa). The HPH led to significantly higher ( P < 0.05) in vitro inhibition of the activities of angiotensin I‐converting enzyme (ACE) and renin, the two main enzymes involved in abnormal blood pressure elevation (hypertension). Kinetic studies showed that HPH and peptide fractions inhibited renin and ACE activities in a mixed‐type pattern, indicating binding to areas other than the active site. Oral administration of HPH (200 mg/kg body weight) to spontaneously hypertensive rats led to significant reductions ( P < 0.05) in systolic blood pressure (SBP) that reached a maximum of −30 mmHg after 8 h. In contrast, the hypotensive effects of peptide fractions (<1 and 1–3 kDa) had a maximum value of about −15 mmHg after 6–8 h post oral administration. The results suggest a synergistic antihypertensive effect of the peptides present within HPH; this effect was reduced significantly ( P < 0.05) upon separation into peptide fractions.

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Background
Amaranth as a Source of Antihypertensive Peptides
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Thermoase-Derived Flaxseed Protein Hydrolysates and Membrane Ultrafiltration Peptide Fractions Have Systolic Blood Pressure-Lowering Effects in Spontaneously Hypertensive Rats
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Peptides present in the non-digestible fraction of common beans (Phaseolus vulgaris L.) inhibit the angiotensin-I converting enzyme by interacting with its catalytic cavity independent of their antioxidant capacity.
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Kinetics Study of Protein Hydrolysis and Inhibition of Angiotensin Converting Enzyme by Peptides Hydrolysate Extracted from Walnut
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Thermoase-hydrolysed pigeon pea protein and its membrane fractions possess in vitro bioactive properties (antioxidative, antihypertensive, and antidiabetic).
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Self-citation

Authors: Girgih, A. T., Udenigwe, C. C., Li, H., Adebiyi, A. P., & Aluko, R. E.
Affiliations: University of Manitoba (CA)
Corresponding author: Aluko, Rotimi E.
ORCID: Girgih, Abraham T.
Udenigwe, Chibuike C.
Li, Huan
Aluko, Rotimi E.
Venue: Journal of the American Oil Chemists' Society
Publisher: Wiley
Published: 2011-05-10
Volume: 88
Issue: 11
Pages: 1767-1774
ISSN: 0003-021X (ISSN-L)
1558-9331
DOI: 10.1007/s11746-011-1841-9
OpenAlex: W2093049132
Cited by: 174
References: 0
Citations by year: 2026: 5 · 2025: 9 · 2024: 12 · 2023: 7 · 2022: 12 · 2021: 15 · 2020: 15 · 2019: 12 · 2018: 11 · 2017: 17 · 2016: 17 · 2015: 11 · 2014: 9 · 2013: 16 · 2012: 4
FWCI: 4.02
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Journal of the American Oil Chemists Society (publishedVersion)
Funders: Natural Sciences and Engineering Research Council of Canada
Research Executive Agency
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
Keywords: Cannabis sativa, antihypertensive peptides, angiotensin-converting enzyme inhibition, renin inhibition, gastrointestinal digestion, spontaneously hypertensive rats, blood pressure reduction, synergistic effect

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Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed ( Cannabis sativa L.) Protein Hydrolysates

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