Inhibitory Effect of Delphinidin on Monocyte–Endothelial Cell Adhesion Induced by Oxidized Low-Density Lipoprotein via ROS/p38MAPK/NF-κB Pathway
Abstract
Monocyte adhesion to the vascular endothelium and their subsequent trans-endothelial migration are pivotal early events in atherogenesis. In this study, the effect of delphinidin, belonging to the group of anthocyanin, on adhesion of monocytes to endothelial cells induced by ox-LDL was investigated. The results showed that the pre-treatment with delphinidin (50, 100, or 200 μM) dose-dependently decreased the ox-LDL-induced up-regulation of the expression of ICAM-1 and P-selectin, and the enhanced adhesion and transmigration of monocytes. To determine the role of ROS/p38MAPK/NF-κB pathway, intracellular ROS level, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein, and mRNA expression were measured. The results showed that delphinidin attenuated ox-LDL-induced generation of ROS, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein and mRNA expression in endothelial cells in a dose-dependent manner. These results suggest that delphinidin attenuates ox-LDL induced expression of adhesion molecules (P-selectin and ICAM-1) and the adhesion of monocytes to endothelial cells by inhibiting ROS/p38MAPK/NF-κB pathway. These findings provide a basis for the design of potent antiatherosclerotic agents that will have therapeutic potential in the prevention of AS.
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Circulating lipids increase NOX2 production in ECs in vitro.
“Circulating lipids also increase NOX2 production in ECs in vitro (159, 208, 856, 1063).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [159]
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- Authors
- Chen, C.-Y., Yi, L., Jin, X., Zhang, T., Fu, Y.-J., Zhu, J.-D., Mi, M.-T., Zhang, Q.-Y., Ling, W.-H., & Yu, B.
- Affiliations
- Army Medical University (CN)
- Venue
- Cell Biochemistry and Biophysics
- Published
- 2011-06-22
- Volume
- 61
- Issue
- 2
- Pages
- 337-348
- ISSN
- 1085-9195 (ISSN-L)
- 1559-0283
- OpenAlex
- W1996708293
- Cited by
- 61
- References
- 54
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 5 · 2022: 3 · 2020: 4 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 7 · 2015: 5 · 2014: 7 · 2013: 9 · 2012: 2
- FWCI
- 3.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Biochemistry and Biophysics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- delphinidin, anthocyanins, monocyte-endothelial cell adhesion, oxidized LDL, reactive oxygen species, p38 MAPK, NF-κB signaling, NADPH oxidase, ICAM-1, P-selectin, atherosclerosis, anti-atherosclerotic agents, endothelial cells
- MeSH terms
- Anthocyanins, Cell Adhesion, Cell Line, Cell Movement, Gene Expression Regulation, Humans, Lipoproteins, LDL, Monocytes, NF-kappa B, Reactive Oxygen Species, Intercellular Adhesion Molecule-1, P-Selectin, MAP Kinase Signaling System, Endothelial Cells, p38 Mitogen-Activated Protein Kinases
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