Oxytocin receptor ligands induce changes in cytoskeleton in neuroblastoma cells
Abstract
Aim of the present study was to evaluate effects of ligands of oxytocin receptors on gene expression of neurofilament proteins (nestin and microtubule-associated protein 2 (MAP2)) associated with neuronal differentiation and growth factors (brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF)) related to neuronal growth. Fluorescent staining of F-actin was used to observe morphology of cells. Co-treatment with oxytocin and oxytocin receptor antagonist--atosiban--resulted in significant increase of MAP2 gene expression in SK-N-SH cells. There was no effect of oxytocin on gene expression of growth factors BDNF and NGF. Surprisingly, oxytocin with atosiban significantly increased mRNA levels for both BDNF and NGF. Gene expression of vasopressin receptor (V1aR) significantly decreased in response to vasopressin. Atosiban decreased mRNA levels for oxytocin receptor (OXTR) and V1aR. Oxytocin significantly decreased OXTR and nestin mRNA levels and increased mRNA levels for BDNF and NGF in U-87 MG cells. The densest recruitment of F-actin filaments was observed in apical parts of filopodia in SK-N-SH cells incubated in oxytocin presence. Present data demonstrate complex role of ligands of oxytocin receptors in regulation of gene expression of intermediate filaments and thus, oxytocin might be considered as a growth factor in neuronal type of cells.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
OT can increase BDNF expression in hippocampus and neuroblastoma cells.
“It has been reported that OT can increase BDNF expression in hippocampus (72) and neuroblastoma cells (73), but otherwise little is known about OT-BDNF interactions.”
From Oxytocin receptors in the anteromedial bed nucleus of the stria terminalis promote stress-induced social avoidance in female California mice (2018) · cites this paper as [73]
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
The oxytocin receptor system: structure, function, and regulation
Gimpl, G., & Fahrenholz, F. (2001) · Physiological Reviews · doi:10.1152/physrev.2001.81.2.629 · Cited by 3,089 · see citing works
Evoked axonal oxytocin release in the central amygdala attenuates fear response
Knobloch, H. S., Charlet, A., Hoffmann, L. C., Eliava, M., Khrulev, S., Cetin, A. H., Osten, P., Schwarz, M. K., Seeburg, P. H., Stoop, R., et al. (2012) · Neuron · doi:10.1016/j.neuron.2011.11.030 · Cited by 1,049 · see citing works
Alderson, R. F., Alterman, A. L., Barde, Y., & Lindsay, R. M. (1990) · Neuron · doi:10.1016/0896-6273(90)90166-d · Cited by 685 · see citing works
Bosch, O. J., & Neumann, I. D. (2012) · Hormones and Behavior · doi:10.1016/j.yhbeh.2011.11.002 · Cited by 400 · see citing works
Central vasopressin and oxytocin release: regulation of complex social behaviours
VEENEMA, A., & NEUMANN, I. (2008) · Progress in Brain Research · doi:10.1016/s0079-6123(08)00422-6 · Cited by 338 · see citing works
Oxytocin stimulates adult neurogenesis even under conditions of stress and elevated glucocorticoids
Leuner, B., Caponiti, J., & Gould, E. (2011) · Hippocampus · doi:10.1002/hipo.20947 · Cited by 237 · see citing works
Theodosis, D. T. (2002) · Frontiers in Neuroendocrinology · doi:10.1006/frne.2001.0226 · Cited by 219 · see citing works
Reif, G. A., Yamaguchi, T., Nivens, E., et al. (2011) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00243.2011 · Cited by 161 · see citing works
Expression of Nestin by Neural Cells in the Adult Rat and Human Brain
Hendrickson, M. L., Rao, A. J., Demerdash, O., & Kalil, R. E. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018535 · Cited by 153 · see citing works
The oxytocin receptor antagonist atosiban inhibits cell growth via a “biased agonist” mechanism
Reversi, A., Rimoldi, V., Marrocco, T., Cassoni, P., Bussolati, G., Parenti, M., & Chini, B. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m409945200 · Cited by 139 · see citing works
Neurotrophin and Neurotrophin Receptor Protein Expression in the Human Lung
Rícci, A., Felici, L., Mariotta, S., Mannino, F., Schmid, G., Terzano, C., Cardillo, G., Amenta, F., & Bronzetti, E. (2003) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2002-0110oc · Cited by 137 · see citing works
Kupershmidt, L., Weinreb, O., Amit, T., Mandel, S. A., Carrı̀, M. T., & Youdim, M. B. H. (2009) · The FASEB Journal · doi:10.1096/fj.09-130047 · Cited by 123 · see citing works
Choi, S. A., Hwang, S.-K., Wang, K.-C., Cho, B.-K., Phi, J. H., Lee, J. Y., Jung, H., Lee, D. H., & Kim, S. (2010) · Neuro-Oncology · doi:10.1093/neuonc/noq147 · Cited by 94 · see citing works
Jin, Z., Liu, L., Bian, W., Chen, Y., Xu, G., Cheng, L., & Jing, N. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m805632200 · Cited by 93 · see citing works
Theodosis, D. T., Trailin, A., & Poulain, D. A. (2006) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00755.2005 · Cited by 77 · see citing works
Cassoni, P., Marrocco, T., Bussolati, B., Allìa, E., Munaron, L., Sapino, A., & Bussolati, G. (2006) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-06-0024 · Cited by 76 · see citing works
PKC delta and NADPH oxidase in retinoic acid-induced neuroblastoma cell differentiation
Nitti, M., Furfaro, A. L., Cevasco, C., Traverso, N., Marinari, U. M., Pronzato, M. A., & Domenicotti, C. (2010) · Cellular Signalling · doi:10.1016/j.cellsig.2010.01.007 · Cited by 72 · see citing works
McLaughlin, D., Tsirimonaki, E., Vallianatos, G., Sakellaridis, N., Chatzistamatiou, T. K., Stavropoulos-Gioka, C., Tsezou, A., Messinis, I. E., & Mangoura, D. A. (2006) · Journal of Neuroscience Research · doi:10.1002/jnr.20828 · Cited by 63 · see citing works
Mune, T., Murase, H., Yamakita, N., Fukuda, T., Murayama, M. A., Miura, A., Suwa, T., Hanafusa, J., Daido, H., Morita, H., et al. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2002-020067 · Cited by 56 · see citing works
Wang, Y., & Hatton, G. I. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.4119-07.2007 · Cited by 51 · see citing works
Che, T., Sun, H. Q., Li, J., Yu, X., Zhu, D., Xue, B., Liu, K., Zhang, M., Kunze, W., & Liu, C. (2012) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2012.07702.x · Cited by 43 · see citing works
Effect of Oxytocin on Neuroblastoma Cell Viability and Growth
Bakoš, J., Štrbák, V., Ratulovska, N., & Bačová, Z. (2012) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-012-9799-1 · Cited by 38 · see citing works
Gogarten, W., Emala, C. W., Lindeman, K. S., et al. (2001) · Biology of Reproduction · doi:10.1095/biolreprod65.2.401 · Cited by 37 · see citing works
Mouse nestin protein localizes in growth cones of P19 neurons and cerebellar granule cells
Yan, Y., Yang, J., Bian, W., et al. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(01)01664-0 · Cited by 30 · see citing works
Finger, E. C. (2011) · Journal of Molecular Neuroscience · doi:10.1007/s12031-011-9550-2 · Cited by 28 · see citing works
Croci, T., Cecchi, R., Marini, P., Rouget, C., Viviani, N., Germain, G., Guagnini, F., Fradin, Y., Descamps, L., Pascal, M., et al. (2007) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.106.119123 · Cited by 26 · see citing works
The cytoskeleton of human myometrial cells
Yu, J. T. H. T., & Bernal, A. L. (1998) · Reproduction · doi:10.1530/jrf.0.1120185 · Cited by 25 · see citing works
Differential Responses of Human Neural and Hematopoietic Stem Cells to Ethanol Exposure
Hao, H., Parker, G. C., Zhao, J. Y., et al. (2003) · Stem Cells and Development · doi:10.1089/152581603322286024 · Cited by 17 · see citing works
Comparative effects of arginine vasopressin and oxytocin in cell culture systems
Briner, V. A., Tsai, P., Choong, H. L., et al. (1992) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.1992.263.2.f222 · Cited by 16 · see citing works
Karibe, H., Matsuo, K., Gokita, T., et al. (1990) · European Journal of Pharmacology Molecular Pharmacology · doi:10.1016/0922-4106(90)90202-9 · Cited by 13 · see citing works
Tahara, A., Tsukada, J., Tomura, Y., et al. (2011) · Neuropeptides · doi:10.1016/j.npep.2010.12.001 · Cited by 13 · see citing works
The Effect of Oxytocin and an Oxytocin Antagonist on the Human Myometrial Proteome
Wit, N. C. J. D., Heck, A. J. R., & Thornton, S. (2009) · Reproductive Sciences · doi:10.1177/1933719109345287 · Cited by 9 · see citing works
Cytotoxicity of copper(II) complexes of N-salicylidene-L-glutamate: modulation by ascorbic acid.
Paulíková, H., Kadlecikova, E., Sucháňová, M., et al. (2008) · PubMed · Cited by 7 · see citing works
Tahara, A., Tsukada, J., Tomura, Y., Yatsu, T., & Shibasaki, M. (2012) · Clinical and Experimental Pharmacology and Physiology · doi:10.1111/j.1440-1681.2012.05693.x · Cited by 6 · see citing works
Bidirectional Communication between Neural and Cardiac Cells in Human Amniotic Fluid
Stefanidis, K., Loutradis, D., Anastasiadou, V., et al. (2009) · Fetal Diagnosis and Therapy · doi:10.1159/000200016 · Cited by 1 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Duque-Wilckens, N., Steinman, M. Q., Busnelli, M., Chini, B., Yokoyama, S., Pham, M., Laredo, S. A., Hao, R., Perkeybile, A. M., Minie, V. A., et al. (2018) · Biological Psychiatry · doi:10.1016/j.biopsych.2017.08.024 · Cited by 181 · see citing works
- Authors
- Bakos, J., Strbak, V., Paulikova, H., Krajnakova, L., Lestanova, Z., & Bacova, Z.
- Affiliations
- Slovak University of Technology in Bratislava (SK)
- Slovak Academy of Sciences (SK)
- Venue
- Journal of Molecular Neuroscience
- Published
- 2013-01-20
- Volume
- 50
- Issue
- 3
- Pages
- 462-468
- ISSN
- 0895-8696 (ISSN-L)
- 1559-1166
- OpenAlex
- W2037102840
- Cited by
- 18
- References
- 36
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2019: 1 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 1
- FWCI
- 3.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Molecular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Slovenská Akadémia Vied
- Agentúra na Podporu Výskumu a Vývoja
- Division of Chemistry
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- oxytocin receptor, neuroblastoma cells, cytoskeleton, F-actin, nestin, MAP2, brain-derived neurotrophic factor, nerve growth factor, atosiban, vasopressin V1a receptor, intermediate filaments, neuronal differentiation, growth factors, filopodia
- MeSH terms
- Actins, Humans, Ligands, Actin Cytoskeleton, Microtubule-Associated Proteins, Neuroblastoma, Neurons, Oxytocin, Transcription, Genetic, Vasotocin, Receptors, Vasopressin, Receptors, Oxytocin, Brain-Derived Neurotrophic Factor, Nerve Growth Factor, Cell Line, Tumor, Nestin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review