Cited by 18Neuroendocrine regulation and behaviorNeuropeptides and Animal PhysiologyNeurogenesis and neuroplasticity mechanisms

Oxytocin receptor ligands induce changes in cytoskeleton in neuroblastoma cells

Bakos, Jan, Strbak, Vladimir, Paulikova, Helena, Krajnakova, Lucia, Lestanova, Zuzana, Bacova, Zuzana·2013·Journal of Molecular Neuroscience

Abstract

Aim of the present study was to evaluate effects of ligands of oxytocin receptors on gene expression of neurofilament proteins (nestin and microtubule-associated protein 2 (MAP2)) associated with neuronal differentiation and growth factors (brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF)) related to neuronal growth. Fluorescent staining of F-actin was used to observe morphology of cells. Co-treatment with oxytocin and oxytocin receptor antagonist--atosiban--resulted in significant increase of MAP2 gene expression in SK-N-SH cells. There was no effect of oxytocin on gene expression of growth factors BDNF and NGF. Surprisingly, oxytocin with atosiban significantly increased mRNA levels for both BDNF and NGF. Gene expression of vasopressin receptor (V1aR) significantly decreased in response to vasopressin. Atosiban decreased mRNA levels for oxytocin receptor (OXTR) and V1aR. Oxytocin significantly decreased OXTR and nestin mRNA levels and increased mRNA levels for BDNF and NGF in U-87 MG cells. The densest recruitment of F-actin filaments was observed in apical parts of filopodia in SK-N-SH cells incubated in oxytocin presence. Present data demonstrate complex role of ligands of oxytocin receptors in regulation of gene expression of intermediate filaments and thus, oxytocin might be considered as a growth factor in neuronal type of cells.

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Crossref · 10.1007/s12031-013-9960-4

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Authors
Bakos, J., Strbak, V., Paulikova, H., Krajnakova, L., Lestanova, Z., & Bacova, Z.
Affiliations
  • Slovak University of Technology in Bratislava (SK)
  • Slovak Academy of Sciences (SK)
Venue
Journal of Molecular Neuroscience
Published
2013-01-20
Volume
50
Issue
3
Pages
462-468
ISSN
  • 0895-8696 (ISSN-L)
  • 1559-1166
OpenAlex
W2037102840
Cited by
18
References
36
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2019: 1 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 1
FWCI
3.99
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Molecular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Slovenská Akadémia Vied
  • Agentúra na Podporu Výskumu a Vývoja
  • Division of Chemistry
Topics
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
oxytocin receptor, neuroblastoma cells, cytoskeleton, F-actin, nestin, MAP2, brain-derived neurotrophic factor, nerve growth factor, atosiban, vasopressin V1a receptor, intermediate filaments, neuronal differentiation, growth factors, filopodia
MeSH terms
Actins, Humans, Ligands, Actin Cytoskeleton, Microtubule-Associated Proteins, Neuroblastoma, Neurons, Oxytocin, Transcription, Genetic, Vasotocin, Receptors, Vasopressin, Receptors, Oxytocin, Brain-Derived Neurotrophic Factor, Nerve Growth Factor, Cell Line, Tumor, Nestin

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