Cited by 2Fungal and yeast genetics researchBacterial Genetics and BiotechnologyAntifungal resistance and susceptibility

The UPC2 Gene in Kluyveromyces lactis Stress Adaptation

Betinova, Veronika, Toth Hervay, Nora, Elias, Daniel, Horvathova, Agnes, Gbelska, Yvetta·2022·Folia Microbiologica

Abstract

KlUpc2p, a transcription factor belonging to the fungal binuclear cluster family, is an important regulator of ergosterol biosynthesis and azole drug resistance in Kluyveromyces lactis. In this work, we show that the absence of KlUpc2p generates Rag- phenotype and modulates the K. lactis susceptibility to oxidants and calcofuor white. The KlUPC2 deletion leads to increased expression of KlMGA2 gene, encoding an important regulator of hypoxic and lipid biosynthetic genes in K. lactis and also KlHOG1 gene. The absence of KlUpc2p does not lead to statistically significant changes in glycerol, corroborating the expression of KlGPD1 gene, encoding NAD+-dependent glycerol-3-phosphate dehydrogenase, that is similar in both the deletion mutant and the parental wild-type strain. Increased sensitivity of Klupc2 mutant cells to brefeldin A accompanied with significant increase in KlARF2 gene expression point to the involvement of KlUpc2p in intracellular signaling. Our observations highlight the connections between ergosterol and fatty acid metabolism to modulate membrane properties and point to the possible involvement of KlUpc2p in K. lactis oxidative stress response.

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Crossref · 10.1007/s12223-022-00968-3

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Authors
Betinova, V., Toth Hervay, N., Elias, D., Horvathova, A., & Gbelska, Y.
Affiliations
Comenius University Bratislava (SK)
Venue
Folia Microbiologica
Published
2022-03-30
Volume
67
Issue
4
Pages
641-647
ISSN
  • 0015-5632 (ISSN-L)
  • 1874-9356
OpenAlex
W4220781932
Cited by
2
References
38
Citations by year
2026: 1 · 2023: 1
FWCI
0.08
Type
journal article
Language
en
Open access
closed
Locations
  • Folia Microbiologica (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Agentúra na Podporu Výskumu a Vývoja (APVV-19-0094)
  • Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR (VEGA 1/0697/18, VEGA 1/0388/22)
  • Univerzita Komenského v Bratislave (UK/62/2021)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
Kluyveromyces lactis, stress adaptation, oxidative stress response, ergosterol biosynthesis, Hog1, glycerol-3-phosphate dehydrogenase, ARF2, brefeldin A sensitivity, membrane properties, fatty acid metabolism, calcofluor white
MeSH terms
Ergosterol, Fungal Proteins, Kluyveromyces, Transcription Factors, Gene Expression Regulation, Fungal, Gene Deletion

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