The UPC2 Gene in Kluyveromyces lactis Stress Adaptation
Abstract
KlUpc2p, a transcription factor belonging to the fungal binuclear cluster family, is an important regulator of ergosterol biosynthesis and azole drug resistance in Kluyveromyces lactis. In this work, we show that the absence of KlUpc2p generates Rag- phenotype and modulates the K. lactis susceptibility to oxidants and calcofuor white. The KlUPC2 deletion leads to increased expression of KlMGA2 gene, encoding an important regulator of hypoxic and lipid biosynthetic genes in K. lactis and also KlHOG1 gene. The absence of KlUpc2p does not lead to statistically significant changes in glycerol, corroborating the expression of KlGPD1 gene, encoding NAD+-dependent glycerol-3-phosphate dehydrogenase, that is similar in both the deletion mutant and the parental wild-type strain. Increased sensitivity of Klupc2 mutant cells to brefeldin A accompanied with significant increase in KlARF2 gene expression point to the involvement of KlUpc2p in intracellular signaling. Our observations highlight the connections between ergosterol and fatty acid metabolism to modulate membrane properties and point to the possible involvement of KlUpc2p in K. lactis oxidative stress response.
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In Kluyveromyces lactis, KIUpc2, whose homolog activates Pdr1 in response to fluconazole in Candida glabrata, has been reported to be involved in the oxidative stress response.
“In Kluyveromyces lactis, KIUpc2, whose homolog activates Pdr1 in response to fluconazole in C. glabrata [46], has been reported to be involved in the oxidative stress response [47].”
From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [47]
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Okamoto, M., Nakano, K., Takahashi-Nakaguchi, A., Sasamoto, K., Yamaguchi, M., Teixeira, M. C., & Chibana, H. (2023) · Journal of Fungi · doi:10.3390/jof9020240 · Cited by 11 · see citing works
“glabrata [46], has been reported to be involved in the oxidative stress response [47].”
Background
- Authors
- Betinova, V., Toth Hervay, N., Elias, D., Horvathova, A., & Gbelska, Y.
- Affiliations
- Comenius University Bratislava (SK)
- ORCID
- Gbelska, Yvetta
- Venue
- Folia Microbiologica
- Published
- 2022-03-30
- Volume
- 67
- Issue
- 4
- Pages
- 641-647
- ISSN
- 0015-5632 (ISSN-L)
- 1874-9356
- OpenAlex
- W4220781932
- Cited by
- 2
- References
- 38
- Citations by year
- 2026: 1 · 2023: 1
- FWCI
- 0.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Folia Microbiologica (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agentúra na Podporu Výskumu a Vývoja (APVV-19-0094)
- Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR (VEGA 1/0697/18, VEGA 1/0388/22)
- Univerzita Komenského v Bratislave (UK/62/2021)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Kluyveromyces lactis, stress adaptation, oxidative stress response, ergosterol biosynthesis, Hog1, glycerol-3-phosphate dehydrogenase, ARF2, brefeldin A sensitivity, membrane properties, fatty acid metabolism, calcofluor white
- MeSH terms
- Ergosterol, Fungal Proteins, Kluyveromyces, Transcription Factors, Gene Expression Regulation, Fungal, Gene Deletion
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