Aciduric Strains of Lactobacillus reuteri and Lactobacillus rhamnosus, Isolated from Human Feces, Have Strong Adhesion and Aggregation Properties
Abstract
Human feces were streaked onto MRS Agar adjusted to pH 2.5, 3.0, and 6.4, respectively, and medium supplemented with 1.0% (w/v) bile salts. Two aciduric strains, identified as Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 (based on 16S rDNA and recA sequences), were non-hemolytic and did not hydrolyze mucin. The surface of Lactobacillus reuteri HFI-LD5 cells has a weak negative charge, whereas Lactobacillus rhamnosus HFI-K2 has acidic and basic properties, and produces exopolysaccharides (EPS). None of the strains produce bacteriocins. Both strains are resistant to several antibiotics, including sulfamethoxazole-trimethoprim and sulphonamides. The ability of Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 to grow at pH 2.5 suggests that they will survive passage through the stomach. EPS production may assist in binding to intestinal mucus, especially in the small intestinal tract, protect epithelial cells, and stimulate the immune system. Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 may be used as probiotics, especially in the treatment of small intestinal bacterial overgrowth (SIBO).
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The ability of Lactobacillaceae to aggregation can form a barrier and may exclude pathogenic strains from adhesion to gastrointestinal tract.
“The ability of Lactobacillaceae to aggregation can form a barrier and may exclude pathogenic strains from adhesion to gastrointestinal tract (Klopper et al. 2018).”
From Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria (2022) · cites this paper as Klopper et al. 2018
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- Authors
- Klopper, K. B., Deane, S. M., & Dicks, L. M. T.
- Affiliations
- Stellenbosch University (ZA)
- Corresponding author
- Dicks, Leon M. T.
- Venue
- Probiotics and Antimicrobial Proteins
- Published
- 2017-07-29
- Volume
- 10
- Issue
- 1
- Pages
- 89-97
- ISSN
- 1867-1306 (ISSN-L)
- 1867-1314
- OpenAlex
- W2740560827
- Cited by
- 53
- References
- 72
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 5 · 2023: 11 · 2022: 7 · 2021: 4 · 2020: 3 · 2019: 7 · 2018: 5
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Probiotics and Antimicrobial Proteins (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus reuteri, Lactobacillus rhamnosus, acid resistance, bile salt tolerance, bacterial adhesion, bacterial aggregation, exopolysaccharide production, cell surface charge, antibiotic resistance, probiotics, small intestinal bacterial overgrowth
- MeSH terms
- Acids, Bacterial Adhesion, Epithelial Cells, Feces, Humans, Intestine, Small, Mucus, Probiotics, Limosilactobacillus reuteri, Lacticaseibacillus rhamnosus
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