Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum
Abstract
To identify bacterial traits related to adhesion ability in human bifidobacteria, 13 strains of Bifidobacterium longum isolated from human gastric juice and intestine were studied. Strains were tested for their capability to adhere to Caco-2 cells and classified as adhesive (Adh+) or non-adhesive (Adh-). Adh+ and Adh- strains were then investigated for their autoaggregation ability and surface hydrophobicity. Comparing the properties of Adh+ and Adh-, we observed that strains were able to adhere to cell monolayers if they autoaggregate and manifest a good degree of hydrophobicity as determined by microbial adhesion to hydrocarbons. These two traits could be used for preliminary screening to identify potentially adherent isolates.
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Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli.
“Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli [8, 12–16].”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [14]
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ADHESION FACTORS OF LACTIC ACID BACTERIA AND BIFIDOBACTERIA
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- Authors
- Del Re, B., Sgorbati, B., Miglioli, M., & Palenzona, D.
- Affiliations
- University of Bologna (IT)
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-12
- Volume
- 31
- Issue
- 6
- Pages
- 438-442
- ISSN
- 0266-8254 (ISSN-L)
- 1365-2673
- OpenAlex
- W2001210103
- Cited by
- 731
- References
- 21
- Citations by year
- 2026: 23 · 2025: 31 · 2024: 49 · 2023: 45 · 2022: 54 · 2021: 68 · 2020: 50 · 2019: 34 · 2018: 52 · 2017: 50 · 2016: 30 · 2015: 43 · 2014: 34 · 2013: 31 · 2012: 25
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Bifidobacterium longum, bacterial adhesion, self-aggregation, surface hydrophobicity, Caco-2 cells, human gut bacteria, adherence screening, Bifidobacterium
- MeSH terms
- Bacterial Adhesion, Bifidobacterium, Humans, Intestines, Species Specificity, Stomach, Tumor Cells, Cultured
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