Probiotic characteristics of Lactiplantibacillus Plantarum N-1 and its cholesterol-lowering effect in Hypercholesterolemic rats
Abstract
In this study, the probiotic potential and treatment effects of Lactiplantibacillus plantarum N-1 in hypercholesterolemic rats were investigated, and the possible regulatory mechanisms of lipid metabolism via short-chain fatty acids (SCFAs) and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase were elucidated. The strain N-1 displayed probiotic properties of antioxidant capacity, adhesion to Caco-2 cells, susceptibility to antibiotics in vitro. The results in animal study showed that the total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels in serum and TC in liver declined significantly in both N-1 and simvastatin (Sta) treatment groups compared to the control (P < 0.05), and the extent of these decreases were similar between them. The expression of the HMG-CoA gene in the N-1 group was downregulated significantly by 31.18% compared to the control (P < 0.01), and the contents of butyrate and valerate in N-1 groups were significantly higher than those in both model and Sta group (P < 0.05). Thus, promoting the production of the intestinal SCFAs and inhibiting the expression of HMG-CoA reductase by L. plantarum N-1 may contribute to the improved lipid metabolism and thus lowering cholesterol level in rats. Our investigation indicated that L. plantarum N-1 has the potential to be developed into a functional food supplement for hypercholesterolemia treatment.
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Short-chain fatty acids derived from Lactobacillus strains inhibit cholesterol synthesis by attenuating the activity of HMG-COA reductase.
“short-chain fatty acids derived from Lactobacillus strains inhibit cholesterol synthesis by attenuating the activity of DL-3-hydroxy-3-methylglutaryl coenzyme A (HMG-COA) reductase (Tian et al., 2022).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Tian et al., 2022
SCFAs play an important role in inhibiting cholesterol synthesis.
“SCFAs, beneficial metabolites resulting from probiotic fermentation, primarily encompass acetic, propionic, and butyric acids, which also play an important role in inhibiting cholesterol synthesis.”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Tian et al., 2022
Probiotics modulate lipid metabolism via two key metabolites, short-chain fatty acids (SCFAs) and secondary bile acids (BAs).
“Probiotics modulate lipid metabolism via two key metabolites, short-chain fatty acids (SCFAs) and secondary bile acids (BAs).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Tian et al., 2022
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Agolino, G., Pino, A., Vaccalluzzo, A., Cristofolini, M., Solieri, L., Caggia, C., & Randazzo, C. L. (2024) · Journal of Functional Foods · doi:10.1016/j.jff.2024.106357 · Cited by 87 · see citing works
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- Authors
- Tian, L., Liu, R., Zhou, Z., Xu, X., Feng, S., Kushmaro, A., Marks, R. S., Wang, D., & Sun, Q.
- Affiliations
- Ben-Gurion University of the Negev (IL)
- Sichuan University (CN)
- Chengdu Medical College (CN)
- Corresponding author
- Wang, Dan
- Sun, Qun
- Venue
- Probiotics and Antimicrobial Proteins
- Published
- 2022-01-22
- Volume
- 14
- Issue
- 2
- Pages
- 337-348
- ISSN
- 1867-1306 (ISSN-L)
- 1867-1314
- OpenAlex
- W4207039223
- Cited by
- 35
- References
- 50
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 14 · 2023: 6 · 2022: 1
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Probiotics and Antimicrobial Proteins (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fondation pour les Etudes et Recherches sur le Développement International (2021YFH0072)
- International Research and Development Program of Sichuan (2021YFH0060, 2019YFH0113)
- National University Student Innovation Experimental Project (201913705030)
- Fundamental Research Funds for the Central Universities (2020CDLZ-17)
- National Key Research and Development Projects (2019YFE0103800)
- Department of Science and Technology of Sichuan Province (2021YFN0092, 2022YFN0021)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Surgery › Natural Products and Biological Research
- Keywords
- Lactobacillus plantarum, probiotic characteristics, hypercholesterolemia, cholesterol-lowering effect, short-chain fatty acids, HMG-CoA reductase, lipid metabolism, butyrate, valerate, antioxidant capacity, Caco-2 cell adhesion, antibiotic susceptibility, simvastatin, functional food supplement, LDL cholesterol, liver cholesterol
- MeSH terms
- Animals, Cholesterol, Humans, Hypercholesterolemia, Caco-2 Cells, Simvastatin, Probiotics, Rats
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