Production of Metabolites in Microalgae Under Alkali Halophilic Growth Medium Using a Dissolved Inorganic Carbon Source
Open accessAbstract
Abstract The production of metabolites in microalgae is influenced by extreme cultivation conditions. Bicarbonate is an inorganic carbon source for phototrophic microalgae culturing. In this study, the effect of dissolved inorganic carbon (DIC) (0.4–13 g L−1) on the accumulation of metabolites in Dunaliella tertiolecta biomass is presented. The highest levels of primary metabolites [lipid (239.6 ± 24.3 mg g−1), protein (336.2 ± 47.5 mg g−1)], secondary metabolites [total phenolic (12.8 ± 2.0 mg g−1), total flavonoid (14.4 ± 2.3 mg g−1), total ascorbate (4.7 ± 1.1 mg g−1)], and pigments [chlorophyll (27.2 ± 3.1 mg g−1), carotenoid (2.0 ± 0.1 mg g−1)] were observed when cells were grown with 5.7 g L−1 of DIC (NaHCO3). The highest biomass concentrations (1.5 ± 0.1 g L−1) were obtained for cells grown in a mixture of DIC (4.3 + 1.1 g L−1, NaHCO3 + Na2CO3). This study recommends the optimal levels of bicarbonate carbon of 5.7 g L−1 for maximising the generation of metabolites in the biomass. It also demonstrates that exogenous excessive DIC in the growth medium would be an effective stressor to produce high-value metabolites in Dunaliella or alkali-halophilic strains. Graphical Abstract
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Supplementation with carbon-mainly in inorganic form-is required to ensure adequate quality of the cultivation medium.
“Supplementation with carbon-mainly in inorganic form-is therefore required to ensure adequate quality of the cultivation medium [126].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [126]
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- Authors
- Roy, U. K., Wagner, J., & Radu, T.
- Affiliations
- Loughborough University (GB)
- Corresponding author
- Wagner, Jonathan
- Radu, Tanja
- Venue
- Waste and Biomass Valorization
- Published
- 2023-01-27
- Volume
- 14
- Issue
- 10
- Pages
- 3339-3354
- ISSN
- 1877-2641 (ISSN-L)
- 1877-265X
- OpenAlex
- W4318217749
- Cited by
- 15
- References
- 45
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 4
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Waste and Biomass Valorization (publishedVersion, cc-by)
- Brunel University Research Archive (BURA) (Brunel University London) (submittedVersion, cc-by)
- Figshare (submittedVersion, cc-by)
- Funders
- Engineering and Physical Sciences Research Council (SGBH FF Feb2019 2)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Dunaliella tertiolecta, microalgae, dissolved inorganic carbon, bicarbonate, metabolite accumulation, lipid accumulation, protein accumulation, phenolic compounds, flavonoids, vitamin C, chlorophyll, carotenoids, biomass, carbon source optimization
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