The role of miRNA-624-5p in congenital hypothyroidism and its molecular mechanism by targeting SIRT1.
Abstract
Background Accumulating reports evidenced that congenital hypothyroidism (CH) is a kind of endocrine diseases caused by thyroid hormone imperfection. MicroRNAs (miRNAs) were confirmed to exhibit protective functions in CH progression. However, the functions and latent mechanism of microRNA-624-5p (miR-624-5p) in CH remains unknown.Objective This report was designed to illustrate the potential molecular mechanisms of miR-624-5p on CH.Methods Rats were induced by 50 mg/day propylthiouracil to conduct CH models. Free thyroxine (fT4) and thyroid-Stimulating hormone (TSH) concentrations were measured to confirm CH model conduction. The direct target of miR-624-5p was predicted and verified by Starbase and dual luciferase reporter assay. Besides, the levels of miR-624-5p and sirtuin1 (SIRT1) in hippocampus or hippocampal neuronal cells were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot assays. Then CH rat behaviors were evaluated using open field test (OFT) and forced swim test (FST). Furthermore, neuronal cells viability and apoptosis were checked using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry.Results qRT-PCR assay suggested that miR-624-5p was up-regulated and SIRT1 was low-expressed in hippocampus tissues of CH rats. SIRT1 was a direct target of miR-624-5p. MiR-624-5p inhibitor signally enhanced fT4 levels and reduced TSH levels in the plasma of CH rats, and improved CH rat depressive behaviors by targeting SIRT1. Moreover, our data also revealed that miR-624-5p inhibitor increased cell viability and reduced apoptotic neuronal cells, which was reversed by silencing of SIRT1.Conclusions Taken together, this research demonstrated that miR-624-5p serves as a promising target for CH treatment.
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In MMD, miRNAs are associated with depression caused by various illnesses and with response to specific treatments such as isoliquiritin, oligonucleotides, or purinergic receptor.
“In MMD, it has been reported that miRNAs are not only involved in the pathogenesis of the disease [17], but are also associated with depression caused by various illnesses [18, 19], as well as the response to specific treatments such as isoliquiritin [20], oligonucleotides [21], or purinergic receptor [22], confirming their potential as disease monitoring biomarkers.”
Cites
3 retracted
Pei, G., Xu, L., Huang, W., & Yin, J. (2020) · International Immunopharmacology · doi:10.1016/j.intimp.2019.106076 · Cited by 26 · see citing works
RetractedChen, X., Gong, X., Jie, J., Yu, W., Chen, X., Du, X., Zhou, Q., & Wu, W. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.27747 · Cited by 13 · see citing works
Self-citationRetractedMeng, T., Shen, S., Cheng, L., & Liu, X. (2019) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2019.8262 · Cited by 5 · see citing works
RetractedAnalysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837 · see citing works
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis
Zhong, G., Long, H., Ma, S., Shunhan, Y., Li, J., & Yao, J. (2019) · Life Sciences · doi:10.1016/j.lfs.2019.03.071 · Cited by 123 · see citing works
Keshavarzi, S., Kermanshahi, S., Karami, L., Motaghinejad, M., Motevalian, M., & Sadr, S. (2019) · NeuroToxicology · doi:10.1016/j.neuro.2019.02.004 · Cited by 120 · see citing works
ROVET, J. F., & Daneman, D. (2003) · Pediatric Drugs · doi:10.2165/00128072-200305030-00001 · Cited by 107 · see citing works
The Role of Micro RNA and Long-Non-Coding RNA in Osteoporosis
Ko, N.-Y., Chen, L.-R., & Chen, K.-H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21144886 · Cited by 83 · see citing works
Luo, Y., Liu, W. Y., Tang, P., Jiang, D., Gu, C., Huang, Y., Gong, F., Rong, Y., Qian, D., Chen, J., et al. (2019) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-019-1491-6 · Cited by 74 · see citing works
Liu, H., Liu, N., Zhao, Y., Zhu, X., Wang, C., Liu, Q., Gao, C., Zhao, X., & Li, J. (2019) · Aging · doi:10.18632/aging.102410 · Cited by 65 · see citing works
Thyroid hormone therapy in congenital hypothyroidism and pediatric hypothyroidism
Bauer, A. J., & Wassner, A. J. (2019) · Endocrine · doi:10.1007/s12020-019-02024-6 · Cited by 56 · see citing works
Lin, C., Wang, S., Chen, Y., et al. (2017) · Cell Death and Disease · doi:10.1038/cddis.2017.354 · Cited by 52 · see citing works
Cordeiro, A., Souza, L. L., Oliveira, L. S. D., Faustino, L. C., Santiago, L. A., Bloise, F. F., Ortiga-Carvalho, T. M., Almeida, N. A. D. S., & Pazos-Moura, C. C. (2012) · Journal of Endocrinology · doi:10.1530/joe-12-0420 · Cited by 43 · see citing works
Tuli, G., Munarin, J., Tessaris, D., Matarazzo, P., Einaudi, S., & SANCTIS, L. D. (2020) · Endocrine · doi:10.1007/s12020-020-02370-w · Cited by 43 · see citing works
Li, D., Hao, X., & Song, Y. (2018) · BioMed Research International · doi:10.1155/2018/6204128 · Cited by 39 · see citing works
Xia, X., Yu, Y., Ye, F., Peng, G., Li, Y. J., & Zhou, X. M. (2020) · Neoplasma · doi:10.4149/neo_2020_190517n441 · Cited by 35 · see citing works
SIRT1 and Estrogen Signaling Cooperation for Breast Cancer Onset and Progression
Liarte, S., Alonso-Romero, J. L., & Nicolás, F. J. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00552 · Cited by 34 · see citing works
Al-Khaldi, A.-H., & Sultan, S. (2019) · BMC Endocrine Disorders · doi:10.1186/s12902-019-0350-y · Cited by 34 · see citing works
Hippocampal neurons with stable excitatory connectivity become part of neuronal representations
Castello-Waldow, T. P., Weston, G., Ulivi, A. F., Chenani, A., Loewenstein, Y., Chen, A., & Attardo, A. (2020) · PLoS Biology · doi:10.1371/journal.pbio.3000928 · Cited by 33 · see citing works
The role of endothelial miRNAs in myocardial biology and disease
Boen, J. R. A., Gevaert, A. B., Keulenaer, G. W. D., Craenenbroeck, E. M. V., & Segers, V. F. M. (2019) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2019.11.151 · Cited by 31 · see citing works
Huang, Z., Wei, P., Gan, L., Li, W., Zeng, T., Qin, C., Chen, Z., & Liu, G. (2021) · Molecular Medicine Reports · doi:10.3892/mmr.2021.11953 · Cited by 29 · see citing works
Diagnosis, Treatment and Outcome of Congenital Hypothyroidism
Vliet, G. V., & Deladoëy, J. (2014) · Endocrine development · doi:10.1159/000363155 · Cited by 28 · see citing works
Chen, L., Jia, J., Zang, Y., et al. (2019) · Neoplasma · doi:10.4149/neo_2018_180811n611 · Cited by 27 · see citing works
Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines
Li, Y., Chen, X., Cui, Y., et al. (2019) · Oncology Letters · doi:10.3892/ol.2019.10063 · Cited by 24 · see citing works
Promotive role of microRNA‑150 in hippocampal neurons apoptosis in vascular dementia model rats
Wei, C., Xu, X., Zhu, H., et al. (2021) · Molecular Medicine Reports · doi:10.3892/mmr.2021.11896 · Cited by 18 · see citing works
Khanjani, N., Ahmadzadeh, A., Bakhtiari, B., & Madadizadeh, F. (2016) · Journal of Pediatric Endocrinology and Metabolism · doi:10.1515/jpem-2016-0010 · Cited by 17 · see citing works
Nedel, C. B., Alchini, R., Zucco, J. K., et al. (2010) · Journal of Neuroscience Research · doi:10.1002/jnr.22481 · Cited by 12 · see citing works
Influence mechanism of miRNA‑144 on proliferation and apoptosis of osteosarcoma cells
Zhang, X., Li, Z., Ji, W., Chen, X., Gao, Q., Li, D., & Qin, H. (2019) · Oncology Letters · doi:10.3892/ol.2019.11197 · Cited by 11 · see citing works
Zhang, X., Song, L.-H., Feng, S.-J., et al. (2019) · Folia Neuropathologica · doi:10.5114/fn.2019.91194 · Cited by 11 · see citing works
Li, W., Song, D., Sun, Y., Lv, Y., & Lv, J. (2018) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2018.6062 · Cited by 9 · see citing works
Samandari-Bahraseman, M. R., Jahanshahi, M., barbariha, S. A., et al. (2018) · Annals of Neurosciences · doi:10.1159/000489551 · Cited by 9 · see citing works
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Wu, Z., Cai, Z., Shi, H., Huang, X., Cai, M., Yuan, K., Huang, P., Shi, G., Yan, T., & Li, Z. (2022) · Aging · doi:10.18632/aging.204030 · Cited by 37 · see citing works
- Authors
- Wu, W., Cao, X., & Wang, Y.
- Corresponding author
- Wang, Yuhong
- ORCID
- Wang, Yuhong
- Venue
- Genes & Genomics
- Published
- 2021-10-05
- Volume
- 44
- Issue
- 9
- Pages
- 1137-1147
- ISSN
- 1976-9571 (ISSN-L)
- 2092-9293
- OpenAlex
- W3202470978
- Cited by
- 4
- References
- 32
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 2
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Update notices
- correction (2025-07-17)
- Open access
- closed
- Licence
- Locations
- Genes & Genomics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Surgery › Thyroid and Parathyroid Surgery
- Keywords
- congenital hypothyroidism, SIRT1, microRNA, hippocampus, neuronal apoptosis, depression-like behavior, propylthiouracil-induced hypothyroidism, thyroid-stimulating hormone, free thyroxine, neuroprotection
- MeSH terms
- Animals, Congenital Hypothyroidism, Thyrotropin, Apoptosis, MicroRNAs, Rats, Sirtuin 1
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