Stoichiometry of the reaction of oxyhemoglobin with nitrite
Abstract
During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO2- 4H+ leads to 4Hb+ + 4NO3- + O2 + 2H2 O (Hb denotes hemoglobin monomer). NO2- binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25 degrees C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.
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High concentrations of oxyhemoglobin usually found in the bloodstream can easily convert nitrite to nitrate.
“the high concentrations of oxyhemoglobin usually found in the bloodstream can easily convert nitrite to nitrate [47,48].”
From Sodium nitrite downregulates vascular NADPH oxidase and exerts antihypertensive effects in hypertension (2011) · cites this paper as [48]
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- Authors
- Kosaka, H., Imaizumi, K., Imai, K., & Tyuma, I.
- Affiliations
- The University of Osaka (JP)
- ORCID
- Imaizumi, Kazuhiko
- Venue
- Biochimica et Biophysica Acta (BBA) - Protein Structure
- Publisher
- Elsevier BV
- Published
- 1979-11
- Volume
- 581
- Issue
- 1
- Pages
- 184-188
- ISSN
- 0005-2795 (ISSN-L)
- OpenAlex
- W2077180688
- Cited by
- 139
- References
- 5
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 1 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochimica et Biophysica Acta (BBA) - Protein Structure (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Keywords
- oxyhemoglobin, nitrite, methemoglobin, hemoglobin oxidation, reaction stoichiometry, non-cooperative binding, pH dependence, nitrate
- MeSH terms
- Hemoglobin A, Humans, Hydrogen-Ion Concentration, Kinetics, Methemoglobin, Nitrites, Oxyhemoglobins
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