Cited by 1,337Liver physiology and pathologyAngiogenesis and VEGF in CancerFibroblast Growth Factor Research

Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth.

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Bussolino, F, Di Renzo, M F, Ziche, M, Bocchietto, E, Olivero, M, Naldini, L, Gaudino, G, Tamagnone, L +2 more·1992·The Journal of cell biology

Abstract

Hepatocyte Growth Factor (HGF, also known as Scatter Factor) is a powerful mitogen or motility factor in different cells, acting through the tyrosine kinase receptor encoded by the MET protooncogene. Endothelial cells express the MET gene and expose at the cell surface the mature protein (p190MET) made of a 50 kD (alpha) subunit disulfide linked to a 145-kD (beta) subunit. HGF binding to endothelial cells identifies two sites with different affinities. The higher affinity binding site (Kd = 0.35 nM) corresponds to the p190MET receptor. Sub-nanomolar concentrations of HGF, but not of a recombinant inactive precursor, stimulate the receptor kinase activity, cell proliferation and motility. HGF induces repairs of a wound in endothelial cell monolayer. HGF stimulates the scatter of endothelial cells grown on three-dimensional collagen gels, inducing an elongated phenotype. In the rabbit cornea, highly purified HGF promotes neovascularization at sub-nanomolar concentrations. HGF lacks activities related to hemostasis-thrombosis, inflammation and endothelial cells accessory functions. These data show that HGF is an in vivo potent angiogenic factor and in vitro induces endothelial cells to proliferate and migrate.

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Crossref · 10.1083/jcb.119.3.629

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  • S. epidermidis association promotes expression of angiogenesis genes Ang and Hgf and tissue repair genes Ceacam1, Grn, Hmox1, Igf1, and Pdgfa.

    “S. epidermidis association promoted the expression of genes associated specifically with angiogenesis, including angiogenin (Ang) and Hgf (47, 48), as well as genes that more broadly promote tissue repair, like Ceacam1, Grn, Hmox1, Igf1, and Pdgfa (49–53) (Fig. 3R).”

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Authors
Bussolino, F., Di Renzo, M. F., Ziche, M., Bocchietto, E., Olivero, M., Naldini, L., Gaudino, G., Tamagnone, L., Coffer, A., & Comoglio, P. M.
Affiliations
  • University of Turin (IT)
  • Department of Genetics, Biology and Medical Chemistry, University of Torino.
Venue
The Journal of cell biology
Published
1992-11-01
Volume
119
Issue
3
Pages
629-641
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W2035174231
Cited by
1,337
References
78
Citations by year
2026: 8 · 2025: 11 · 2024: 20 · 2023: 13 · 2022: 19 · 2021: 24 · 2020: 27 · 2019: 22 · 2018: 38 · 2017: 30 · 2016: 40 · 2015: 47 · 2014: 50 · 2013: 50 · 2012: 47
FWCI
22.99
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Archivio Istituzionale della Ricerca - Università Vita-Salute San Raffaele (submittedVersion)
  • Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
  • Use Siena air (University of Siena) (submittedVersion)
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
Keywords
hepatocyte growth factor, angiogenesis, endothelial cell motility, endothelial cell proliferation, c-Met, receptor tyrosine kinase, receptor binding affinity, wound healing, neovascularization, cornea, cell migration
MeSH terms
Animals, Cell Line, Cell Movement, Cells, Cultured, Cornea, Endothelium, Vascular, Humans, Kinetics, Neovascularization, Pathologic, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogenes, Rabbits, Recombinant Proteins, Umbilical Veins, Wound Healing, Mutagenesis, Site-Directed, Hepatocyte Growth Factor, Proto-Oncogene Proteins c-met, Macromolecular Substances, Mice

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