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Cited by 175Skin and Cellular Biology ResearchNeurogenesis and neuroplasticity mechanismsNuclear Structure and Function

The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve

Pachter, Joel S., Liem, Ronald K.H.·1984·Developmental Biology
DOI 10.1016/0012-1606(84)90021-6
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Abstract

The ontogenetic appearance of the individual triplet polypeptides that comprise mammalian neurofilaments was studied in the developing rat optic nerve. Triton-insoluble cytoskeletal preparations from the optic nerves of rats of postnatal ages 1 Day (P1), 6 days (P6), 10 days (P10), 20 days (P20), and 3 months (adult) were analyzed for protein composition by one and two-dimensional gel electrophoresis. Results indicate that at P1, both the 150- and 68-kDa neurofilament subunit proteins are present. The 200-kDa subunit first becomes discernible at P20, but, at this age, it is still present in considerably less quantity than in the adult. Immunocytochemical verification of the presence of neurofilament protein was accomplished by staining tissue sections with specific antibodies against the 150- and the 68-kDa neurofilament subunits using the peroxidase-antiperoxidase technique. Results of the morphological analyses have shown that neurofilaments are not present in quantity until P10, which coincides with the time when the 68-kDa subunit increases in quantity by one dimensional gel analysis. Thus, the 150- and 68-kDa subunits can be detected prior to the appearance of neurofilaments, and the 200-kDa protein is not observed until sometime later. The potential physiological significance of the differential subunit transport is discussed with respect to neuronal differentiation in the developing mammalian CNS.

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Crossref · 10.1016/0012-1606(84)90021-6

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  • Microtubules are apt to be especially important in neuronal development because they arise early, usually much before neurofilaments, and because they have been implicated in outgrowth of neuronal processes and transport within axons and dendrites.

    “In this regard, microtubules are apt to be especially important because they arise early in neuronal development, usually much before neurofilaments (Pachter and Liem, 1984;Peters and Vaughn, 1967;Shaw et al., 1985), and because they have been implicated in a variety of cellular events, including the outgrowth of neuronal processes and the transport of materials within axons and dendrites.”

    From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Pachter and Liem, 1984

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Authors
Pachter, J. S., & Liem, R. K. H.
Affiliations
New York University (US)
Corresponding author
Liem, Ronald K.H.
Venue
Developmental Biology
Publisher
Elsevier BV
Published
1984-05
Volume
103
Issue
1
Pages
200-210
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/0012-1606(84)90021-6
OpenAlex
W1990069940
Cited by
175
References
38
Citations by year
2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2017: 3 · 2015: 2 · 2012: 2
FWCI
6.77
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
neurofilaments, neurofilament subunits, optic nerve development, postnatal development, cytoskeleton, neuronal differentiation, central nervous system development, rats
MeSH terms
Aging, Animals, Animals, Newborn, Cytoskeleton, Electrophoresis, Polyacrylamide Gel, Immunoassay, Intermediate Filament Proteins, Microscopy, Electron, Microtubules, Molecular Weight, Optic Nerve, Rats, Inbred Strains, Neurofilament Proteins, Rats

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