The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Molecular cloning and characterization of human endothelial nitric oxide synthase
Open accessAbstract
The constitutive calcium/calmodulin‐dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase. However, recent cloning and molecular characterization of NO synthase from bovine endothelial cells indicated the existence of a family of constitutive NO synthases. Accordingly, we undertook molecular cloning and sequence analysis of human endothelial NO synthase. Complementary DNA clones predict a protein of 1,203 amino acids sharing 94% identity with the bovine endothelial protein, but only 60% identity with the rat brain NO synthase isoform. Northern blot analysis with an endothelial‐derived cDNA identified a 4.6–4.8 kb mRNA transcript in HUVEC and in situ hybridization localized transcripts to vascular endothelium but not neuronal tissue.
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Cites
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Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse Macrophages
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Cited by
Nitric oxide synthases: structure, function and inhibition
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Nitric oxide synthases in mammals
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Acylation Targets Endothelial Nitric-oxide Synthase to Plasmalemmal Caveolae
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Self-citationFleming, I., Fisslthaler, B., Dimmeler, S., Kemp, B. E., & Busse, R. (2001) · Circulation Research · doi:10.1161/hh1101.092677 · Cited by 770
The nitric oxide and cGMP signal transduction system: regulation and mechanism of action
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Metadata
- Authors
- Marsden, P. A., Schappert, K. T., Chen, H. S., Flowers, M., Sundell, C. L., Wilcox, J. N., Lamas, S., & Michel, T.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1992-08-03
- Volume
- 307
- Issue
- 3
- Pages
- 287-293
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1987149804
- Cited by
- 489
- References
- 20
- FWCI
- 21.12
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Marsden, P. A., Schappert, K. T., Chen, H. S., Flowers, M., Sundell, C. L., Wilcox, J. N., Lamas, S., & Michel, T. (1992). Molecular cloning and characterization of human endothelial nitric oxide synthase. FEBS Letters. https://doi.org/10.1016/0014-5793(92)80697-f
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