Glycogen synthase kinase 3β is identical to tau protein kinase I generating several epitopes of paired helical filaments
Open accessAbstract
We previously reported that tau protein kinase I (TPKI) induced normal tau protein into a state of paired helical filaments (PHF); this is further confirmed here by immunoblot analysis using several antibodies. We also present the amino acid sequence of TPKI, which is identical to glycogen synthase kinase 3β (GSK3β). Moreover, we found that TPKI activity was inseparable from GSK3 activity throughout the purification procedure. These results indicate that TPKI is identical to GSK3β.
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GSK3 is a proline-directed kinase that contributes to phosphorylation of tau at SP/TP sites.
“GSK3 is a proline-directed kinase that contributes to phosphorylation of tau at serine or threonine followed by proline (i.e., SP/TP sites) [46–49]”
From Stabilization of Microtubule-Unbound Tau via Tau Phosphorylation at Ser262/356 by Par-1/MARK Contributes to Augmentation of AD-Related Phosphorylation and Aβ42-Induced Tau Toxicity (2016) · cites this paper as [47]
The Results identify GSK3β as TPKI and report that TPKI phosphorylates tau at specific serine and threonine residues, supporting its role in tau phosphorylation. The passages do not identify those residues as SP/TP sites or describe GSK3β as proline-directed, so that specificity is not established by our copy. (LLM, conf=0.83)
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- Authors
- Ishiguro, K., Shiratsuchi, A., Sato, S., Omori, A., Arioka, M., Kobayashi, S., Uchida, T., & Imahori, K.
- Affiliations
- The University of Tokyo (JP)
- Nihon University (JP)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1993-07-05
- Volume
- 325
- Issue
- 3
- Pages
- 167-172
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2073012903
- Cited by
- 402
- References
- 1
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 9 · 2021: 8 · 2020: 11 · 2019: 11 · 2018: 12 · 2017: 7 · 2016: 13 · 2015: 11 · 2014: 16 · 2013: 12 · 2012: 14
- FWCI
- 12.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- glycogen synthase kinase-3β, tau protein, paired helical filaments, protein phosphorylation, antigenic determinants, tauopathy
- MeSH terms
- Amino Acid Sequence, Animals, Epitopes, Base Sequence, Cattle, DNA, Humans, Molecular Sequence Data, Protein Kinases, Substrate Specificity, Immunoblotting, Protein Serine-Threonine Kinases, Calcium-Calmodulin-Dependent Protein Kinases, Glycogen Synthase Kinases, Glycogen Synthase Kinase 3, Rats
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