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Normal-phase high-performance liquid chromatographic separation of procyanidins from cacao beans and grape seeds
Open accessAbstract
Abstract An HPLC method using a normal-phase silica column and a gradient of dichloromethane—methanol—formic acid—water mixtures as the eluent was developed to separate procyanidins on a molecular mass basis, without derivatization. It was successfully applied to the analysis of procyanidin extracts from cacao beans and grape seeds. The monomers and major dimers were resolved as discrete peaks. Oligomeric and polymeric components were eluted in order of increasing degree of polymerization, as confirmed by determining the average molecular mass of successive fractions collected from the normal-phase column using gel permeation chromatography, after acetylation.
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Cited by
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Awika, J. M., & Rooney, L. W. (2004) · Phytochemistry · doi:10.1016/j.phytochem.2004.04.001 · Cited by 966
Interactions between Polyphenols and Macromolecules: Quantification Methods and Mechanisms
Le Bourvellec, C., & Renard, C. M. G. C. (2012) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2010.499808 · Cited by 853
Hammerstone, J. F., Lazarus, S. A., Mitchell, A. E., Rucker, R., & Schmitz, H. H. (1999) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980760h · Cited by 455
Gu, L., Kelm, M., Hammerstone, J. F., Beecher, G., Cunningham, D., Vannozzi, S., & Prior, R. L. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020214v · Cited by 417
Guyot, S., Marnet, N., Laraba, D., Sanoner, P., & Drilleau, J.-F. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf970832p · Cited by 313
Metadata
- Authors
- Rigaud, J., Escribano-Bailon, M. T., Prieur, C., Souquet, J.-M., & Cheynier, V.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 1993-11
- Volume
- 654
- Issue
- 2
- Pages
- 255-260
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W1998735940
- Cited by
- 159
- References
- 0
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Normal-phase high-performance liquid chromatographic separation of procyanidins from cacao beans and grape seeds. https://app.cochat.ai/papers/10.1016/0021-9673(93)83368-3
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- Rigaud, J., Escribano-Bailon, M. T., Prieur, C., Souquet, J.-M., & Cheynier, V. (1993). Normal-phase high-performance liquid chromatographic separation of procyanidins from cacao beans and grape seeds. Journal of Chromatography A. https://doi.org/10.1016/0021-9673(93)83368-3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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