Cited by 455Food Chemistry and Fat AnalysisPhytochemicals and Antioxidant ActivitiesAnalytical Chemistry and Chromatography

Identification of Procyanidins in Cocoa (Theobroma cacao) and Chocolate Using High-Performance Liquid Chromatography/Mass Spectrometry

Hammerstone, John F., Lazarus, Sheryl A., Mitchell, Alyson E., Rucker, Robert, Schmitz, Harold H.·1999·Journal of Agricultural and Food Chemistry

Abstract

Monomeric and oligomeric procyanidins present in cocoa and chocolate were separated and identified using a modified normal-phase high-performance liquid chromatography (HPLC) method coupled with on-line mass spectrometry (MS) analysis using an atmospheric pressure ionization electrospray chamber. The chromatographic separation was achieved using a silica stationary phase in combination with a gradient ascending in polarity. This qualitative report confirms the presence of a complex series of procyanidins in raw cocoa and certain chocolates using HPLC/MS techniques. Although both cocoa and chocolate contained monomeric and oligomeric procyanidin units 2-10, only use of negative mode provided MS data for the higher oligomers (i.e., >pentamer). Application of this method for qualitative analysis of proanthocyanidins in other food products and confirmation of this method as a reliable quantitative tool for determining levels of procyanidins in cocoa, chocolate, and other food products are currently being investigated.

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Crossref · 10.1021/jf980760h

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  • Macrophages accumulate in adipose tissue in obesity and may be major contributors to adipokine production.

    “It is now recognised that macrophages accumulate in the adipose tissue in obesity (10,11) (Fig. 1) and that these may represent major contributors to the production of adipokines (12,13) .”

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Authors
Hammerstone, J. F., Lazarus, S. A., Mitchell, A. E., Rucker, R., & Schmitz, H. H.
Affiliations
University of California, Davis (US)
Corresponding author
Hammerstone, John F.
Venue
Journal of Agricultural and Food Chemistry
Published
1999-01-14
Volume
47
Issue
2
Pages
490-496
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W1997922496
Cited by
455
References
31
Citations by year
2026: 1 · 2025: 4 · 2024: 2 · 2023: 7 · 2022: 6 · 2021: 7 · 2020: 15 · 2019: 15 · 2018: 18 · 2017: 15 · 2016: 19 · 2015: 24 · 2014: 30 · 2013: 25 · 2012: 33
FWCI
46.99
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Chemistry and Fat Analysis
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
Keywords
procyanidins, Theobroma cacao, chocolate, proanthocyanidins, oligomeric procyanidins, normal-phase HPLC, negative ion mode, cocoa flavonoids
MeSH terms
Cacao, Catechin, Chromatography, High Pressure Liquid, Mass Spectrometry, Proanthocyanidins, Biflavonoids

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