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Cited by 198T-cell and Retrovirus StudiesHIV Research and TreatmentVirus-based gene therapy research

Sequence and spacing requirements of a retrovirus integration site

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Colicelli, John, Goff, Stephen P.·1988·Journal of Molecular Biology
DOI 10.1016/0022-2836(88)90378-6
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Abstract

Following infection, retroviruses insert a DNA copy of their RNA genome into the host cell genome. This integrative recombination reaction occurs at specific sites on the viral DNA: inverted repeat sequences near the termini of the linear DNA form of the viral genome. We have described elsewhere the generation and analysis of deletion mutations at one of the inverted repeat sequences in Moloney murine leukemia virus. We describe here the effects of insertion mutations made at this locus. Our results show that substantial sequence changes at the site of recombination can be tolerated, and that the spacing between the cleavage sites on the viral DNA can be expanded as well as contracted while still allowing efficient viral integration. After several rounds of virus replication, each of the insertion mutants gave rise to pseudorevertants with new alterations at the integration site.

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Crossref · 10.1016/0022-2836(88)90378-6

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  • Depending on the retrovirus type, IN acts on approximately 16-20 terminal nucleotides of the LTR.

    “Depending upon the retrovirus type, IN acts on ~16–20 terminal nucleotides of the LTR (Colicelli and Goff 1988, Withers-Ward et al. 1994, Masuda et al. 1998, Brown et al. 1999).”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Colicelli and Goff 1988

  • The cis-acting att sites are present as imperfect inverted repeats and are both necessary and sufficient for integration in vitro and in vivo.

    “These cis-acting att sites are present in the form of imperfect inverted repeats and are both necessary and sufficient for integration both in vitro and in vivo (Fig. 7) (Colicelli and Goff 1988, Katzman et al. 1989, Bushman et al. 1990, Murphy et al. 1993, Brown et al. 1999).”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Colicelli and Goff 1988

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Authors
Colicelli, J., & Goff, S. P.
Affiliations
Columbia University (US)
ORCID
Goff, Stephen P.
Venue
Journal of Molecular Biology
Publisher
Elsevier BV
Published
1988-01
Volume
199
Issue
1
Pages
47-59
ISSN
0022-2836 (ISSN-L)
DOI
10.1016/0022-2836(88)90378-6
OpenAlex
W2041126567
Cited by
198
References
51
Citations by year
2025: 2 · 2022: 2 · 2021: 1 · 2018: 3 · 2017: 2 · 2015: 2 · 2014: 8 · 2013: 2 · 2012: 7
FWCI
5.37
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Molecular Biology (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
National Cancer Institute (CA 30488)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords
retroviral integration, Moloney murine leukemia virus, inverted repeats, integration sites, insertion mutation, recombination, pseudorevertants, viral DNA
MeSH terms
Base Sequence, DNA, Circular, DNA, Viral, Genes, Viral, Molecular Sequence Data, Mutation, Phenotype, Recombination, Genetic, Retroviridae, Virus Replication

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