Cited by 92Pancreatic function and diabetesDiabetes Treatment and ManagementNeuropeptides and Animal Physiology

Heterogeneity of plasma glucagon immunoreactivity in normal, depancreatized, and alloxan-diabetic dogs

Valverde, Isabel, Dobbs, Richard, Unger, Roger H.·1975·Metabolism

Abstract

Filtration of basal plasma from normal, alloxan-diabetic, and depancreatized dogs on Bio Gel P-10 yielded four glucagon-immunoreactive fractions. One of them appeared in the true glycagon area with the glucagon-125I (3500 mol vt). Of the other three, one appeared in the void volume (greater than 20000 mol wt), another just before the insulin-125I (congruent to 9000 mol wt), and the last one close to the salt peak (less than 2000 mol wt). The increase of total plasma glucagon immunoreactivity observed in depancreatized and alloxan diabetic dogs was mainly due to an increase in the 3500 and 9000 molecular-weight fractions. Arginine infusion in depancreatized dogs caused an increase in the 3500 molecular-weight fraction. Somatostatin or insulin infusion in depancreatized and alloxan-diabetic dogs resulted in disappearance of the 3500 molecular-weight fraction.

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Crossref · 10.1016/0026-0495(75)90095-5

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Authors
Valverde, I., Dobbs, R., & Unger, R. H.
Affiliations
United States Department of Veterans Affairs (US)
Corresponding author
Valverde, Isabel
Venue
Metabolism
Publisher
Elsevier BV
Published
1975-09
Volume
24
Issue
9
Pages
1021-1028
ISSN
0026-0495 (ISSN-L)
OpenAlex
W1991729404
Cited by
92
References
12
Citations by year
2021: 1
FWCI
27.32
Type
journal article
Language
en
Open access
closed
Locations
  • Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
plasma glucagon, molecular weight fractions, depancreatization, alloxan-induced diabetes, arginine infusion, somatostatin, insulin, phloridzin, hypoglycemia
MeSH terms
Animals, Arginine, Chromatography, Gel, Diabetes Mellitus, Experimental, Dogs, Glucagon, Insulin, Iodine Radioisotopes, Molecular Weight, Pancreas, Pancreatectomy, Phlorhizin, Somatostatin

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