Heterogeneity of plasma glucagon immunoreactivity in normal, depancreatized, and alloxan-diabetic dogs
Abstract
Filtration of basal plasma from normal, alloxan-diabetic, and depancreatized dogs on Bio Gel P-10 yielded four glucagon-immunoreactive fractions. One of them appeared in the true glycagon area with the glucagon-125I (3500 mol vt). Of the other three, one appeared in the void volume (greater than 20000 mol wt), another just before the insulin-125I (congruent to 9000 mol wt), and the last one close to the salt peak (less than 2000 mol wt). The increase of total plasma glucagon immunoreactivity observed in depancreatized and alloxan diabetic dogs was mainly due to an increase in the 3500 and 9000 molecular-weight fractions. Arginine infusion in depancreatized dogs caused an increase in the 3500 molecular-weight fraction. Somatostatin or insulin infusion in depancreatized and alloxan-diabetic dogs resulted in disappearance of the 3500 molecular-weight fraction.
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Because the glucagon antiserum crossreacts with species in addition to 3,500-dalton glucagon and somatostatin suppresses 3,500-dalton glucagon preferentially, biologically active glucagon was likely suppressed substantially.
“However, since the antiserum used crossreacts with species in addition to 3,500dalton glucagon (25), and somatostatin suppresses 3,500-dalton glucagon preferentially (26–28), biologically active glucagon was likely suppressed substantially.”
From Epinephrine supports the postabsorptive plasma glucose concentration and prevents hypoglycemia when glucagon secretion is deficient in man (1984) · cites this paper as [26]
Somatostatin preferentially suppresses the secretion of 3,500-D glucagon in dogs, a glucagonoma patient, and normal humans.
“Since somatostatin preferentially suppresses the secretion of 3,500-D glucagon in dogs (20, 21), a glucagonoma patient (22), and normal humans¹”
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- Authors
- Valverde, I., Dobbs, R., & Unger, R. H.
- Affiliations
- United States Department of Veterans Affairs (US)
- Corresponding author
- Valverde, Isabel
- Venue
- Metabolism
- Publisher
- Elsevier BV
- Published
- 1975-09
- Volume
- 24
- Issue
- 9
- Pages
- 1021-1028
- ISSN
- 0026-0495 (ISSN-L)
- OpenAlex
- W1991729404
- Cited by
- 92
- References
- 12
- Citations by year
- 2021: 1
- FWCI
- 27.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- plasma glucagon, molecular weight fractions, depancreatization, alloxan-induced diabetes, arginine infusion, somatostatin, insulin, phloridzin, hypoglycemia
- MeSH terms
- Animals, Arginine, Chromatography, Gel, Diabetes Mellitus, Experimental, Dogs, Glucagon, Insulin, Iodine Radioisotopes, Molecular Weight, Pancreas, Pancreatectomy, Phlorhizin, Somatostatin
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