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Cited by 101

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Glucocorticoid-enhanced neoplastic transformation of human keratinocytes by human papillomavirus type 16 and an activated ras oncogene

Durst, Matthias, Gallahan, Dan, Jay, Gilbert, Rhim, Johng S.·1989·Virology
DOI 10.1016/0042-6822(89)90595-3
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Abstract

Human papillomaviruses (HPV) are known etiological agents of benign proliferation of the skin and mucosa (papillomas and warts). They have also been implicated in the development of cervical dysplasia and anogenital carcinoma. The close association of HPV type 16 DNA with the majority of cervical carcinomas suggests the involvement of the virus in this type of cancer. We have developed an in vitro multistep model for human epithelial cell carcinogenesis. Primary human epidermal keratinocytes acquired an indefinite lifespan in response to transfection with HPV 16 DNA but did not undergo malignant conversion. Addition of Kirsten murine sarcoma virus (Ki-MSV), which contains an activated K-ras oncogene, to these cells induced morphological alterations associated with the acquisition of neoplastic properties. The frequency of transformation by Ki-MSV was markedly enhanced by the inclusion of glucocorticoid. At optimal conditions, a 125-fold stimulation was observed. These findings demonstrate the malignant conversion of human primary epithelial cells in culture by the cooperation of HPV type 16 and an activated ras oncogene and support a multistep process for neoplastic conversion. The availability of a human epithelial cell transformation model should facilitate studies of the interaction between HPV and human epithelial cells.

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Citations

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Show all 1 references

Research works printed but not linked: 25

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Metadata

Authors
Durst, M., Gallahan, D., Jay, G., & Rhim, J. S.
Venue
Virology
Publisher
Elsevier BV
Published
1989-12
Volume
173
Issue
2
Pages
767-771
ISSN
0042-6822
DOI
10.1016/0042-6822(89)90595-3
Cited by
101
References
1
Language
en
Licence

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CoChat. (2026). Citation check: Glucocorticoid-enhanced neoplastic transformation of human keratinocytes by human papillomavirus type 16 and an activated ras oncogene. https://app.cochat.ai/papers/10.1016/0042-6822(89)90595-3
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Durst, M., Gallahan, D., Jay, G., & Rhim, J. S. (1989). Glucocorticoid-enhanced neoplastic transformation of human keratinocytes by human papillomavirus type 16 and an activated ras oncogene. Virology. https://doi.org/10.1016/0042-6822(89)90595-3