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Cited by 101Mast cells and histamineAllergic Rhinitis and SensitizationAsthma and respiratory diseases

Direct in vivo evidence for mast cell degranulation during allergen-induced reactions in man

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Gomez, E., Corrado, O.J., Baldwin, D.L., Swanston, A.R., Davies, R.J.·1986·Journal of Allergy and Clinical Immunology
DOI 10.1016/0091-6749(86)90082-5
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Abstract

There is little or no direct in vivo evidence in man to support the involvement of mast cell-mediator release in the pathogenesis of immediate allergic reactions. We have performed a within-subject controlled study to determine the changes that occur in nasal mast cells during allergen-induced rhinitis. Twelve subjects with asymptomatic rhinitis were studied. Nasal biopsy specimens were obtained from each subject after a control solution (isotonic saline, 0.9% w/v) had been nebulized into one nostril and allergen solution (freeze-dried allergen extract reconstituted with isotonic saline) into the other. The tissues obtained were fixed in Carnoy's solution and stained with the alpha-naphthol AS-D chloroacetate esterase reaction (N AS-D CA ER). Mast cells were counted under light microscopy in the epithelium and lamina propria, and the integrity of each cell was assessed. No significant differences were found in the number of epithelial or lamina propria mast cells in biopsy specimens obtained after saline or allergen administration. However, the number of degranulated mast cells after allergen provocation (89%) was significantly greater than after instillation of control solution (15%) (p = 0.003). Changes of mast cell degranulation after allergen provocation were confirmed by electron microscopy. In six nonatopic, control subjects without rhinitis, there was no significant difference between the percentages of degranulated mast cells after allergen provocation (25.8%) and instillation of saline (24.3%). This study provides direct in vivo evidence for allergen-induced mast cell activation in man.

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  • Degranulation of mast cells and basophils occurs during early and late phases of a type I reaction after allergen encounter and IgE crosslinking, as well as upon IL-33 stimulation.

    “Degranulation of both mast cells381 and basophils occurs during the early and late phases of a type I reaction after allergen encounter and crosslinking of IgE molecules as well as upon stimulation by IL-33.382”

    From International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis (2018) · cites this paper as [381]

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Research works printed but not linked: 1

  1. I. Mygind N: Nasal allergy, ed 2. Oxford, 1979, Blackwell Sci- entific Publications Ltd, pp 219

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Authors
Gomez, E., Corrado, O. J., Baldwin, D. L., Swanston, A. R., & Davies, R. J.
Affiliations
St Bartholomew's Hospital (GB)
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
1986-10
Volume
78
Issue
4
Pages
637-645
ISSN
0091-6749 (ISSN-L)
DOI
10.1016/0091-6749(86)90082-5
OpenAlex
W2101137099
Cited by
101
References
38
Citations by year
2025: 1 · 2023: 2 · 2018: 1 · 2017: 1 · 2014: 1 · 2013: 2
FWCI
2.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
Keywords
mast cell degranulation, allergic rhinitis, allergen challenge, immediate hypersensitivity, mast cell activation, nasal biopsy
MeSH terms
Adult, Allergens, Epithelium, Female, Glutaral, Humans, Male, Mast Cells, Rhinitis, Allergic, Perennial

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