Regulation of terminal differentiation of cultured human keratinocytes by vitamin A
Abstract
Vitamin A is known to exert an important influence on epithelial differentiation. The fetal calf serum supplement of cell-culture medium contains enough of the vitamin to affect the differentiation of cultured keratinocytes derived from epidermis and from other stratified squamous epithelia. The cellular and molecular properties of the cultures are altered when the medium is supplemented with serum from which the vitamin A has been removed by solvent extraction (delipidized serum). Cell motility is reduced, the adhesiveness of cells increases and pattern formation is prevented. In both epidermal and conjunctival keratinocytes, removal of vitamin A leads to the synthesis of a 67 kd keratin characteristic of terminally differentiating epidermis and to much reduced synthesis of the 52 kd and 40 kd keratins typical of conjunctiva. These changes, both cellular and molecular, are reversed by the addition of retinyl acetate to the medium containing delipidized serum. Cell motility and pattern formation are restored, and detachment of the most mature cells from the surface of the stratified epithelium is promoted. Synthesis of the 67 kd keratin is prevented and the synthesis of the 40 and 52 kd keratins is stimulated. The nature of the keratins synthesized is regulated by the concentration of vitamin A, and each cell type adjusts its synthesis differently at a given vitamin concentration.
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Retinoids are the most common anti-aging drugs used to treat and prevent photo-aging of the skin.
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From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [97]
Retinoic acid prevents skin aging by regulating genes and MMPs.
“Retinoids are the most common anti-aging drugs that have been used (such as retinoic acid prevents skin aging by regulating genes and MMPs) to treat and prevent photo-aging of the skin [97,98].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [97]
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- Authors
- Fuchs, E., & Green, H.
- Affiliations
- University of Chicago (US)
- Harvard University (US)
- ORCID
- Fuchs, Elaine
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1981-09
- Volume
- 25
- Issue
- 3
- Pages
- 617-625
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1983851236
- Cited by
- 750
- References
- 49
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 4 · 2020: 8 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 5 · 2015: 7 · 2014: 4 · 2013: 6 · 2012: 4
- FWCI
- 19.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Keywords
- vitamin A, keratinocyte differentiation, terminal differentiation, keratin synthesis, retinyl acetate, cell motility, cell adhesiveness, pattern formation, epidermal keratinocytes, stratified squamous epithelium, epithelial differentiation
- MeSH terms
- Cell Aggregation, Cell Differentiation, Cells, Cultured, Epithelial Cells, Humans, Keratins, Molecular Weight, RNA, Messenger, Vitamin A
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