Cited by 117Cholesterol and Lipid MetabolismLipoproteins and Cardiovascular HealthLipid metabolism and disorders

Overexpression of low density lipoprotein (LDL) receptor eliminates LDL from plasma in transgenic mice

Hofmann, Sandra L., Russell, David W., Brown, Michael S., Goldstein, Joseph L., Hammer, Robert E.·1988·Science

Abstract

A complementary DNA encoding the human low density lipoprotein (LDL) receptor under control of the mouse metallothionein-I promoter was injected into fertilized mouse eggs, and a strain of mice expressing high levels of LDL receptors was established. After administration of cadmium, these mice cleared intravenously injected 125I-labeled LDL from blood eight to ten times more rapidly than did normal mice. The plasma concentrations of apoproteins B-100 and E, the two ligands for the LDL receptor, declined by more than 90 percent after cadmium treatment, but the concentration of another apoprotein, A-I, was unaffected. Therefore, overexpression of an endocytotic receptor can dramatically lower the ambient concentration of its ligand in vivo.

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Crossref · 10.1126/science.3344433

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28 of 28 references verified0 citation errors · 0 not checkable

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Authors
Hofmann, S. L., Russell, D. W., Brown, M. S., Goldstein, J. L., & Hammer, R. E.
Affiliations
The University of Texas Southwestern Medical Center (US)
Venue
Science
Published
1988-03-11
Volume
239
Issue
4845
Pages
1277-1281
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2081393029
Cited by
117
References
28
Citations by year
2023: 2 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2013: 1 · 2012: 1
FWCI
15.95
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL-20948, HL 01287)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
Keywords
LDL receptor, transgenic mice, metallothionein-I promoter, cadmium, LDL clearance, apolipoprotein B-100, apolipoprotein E, apolipoprotein A-I, endocytic receptor, ligand concentration, lipoprotein metabolism
MeSH terms
Animals, Cloning, Molecular, DNA, Female, Genes, Humans, Kinetics, Lipoproteins, LDL, Mice, Transgenic, Plasmids, Receptors, LDL, Reference Values, Transcription, Genetic, Mice

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