Cited by 1,287Hematopoietic Stem Cell Transplantationmelanin and skin pigmentationMast cells and histamine

The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene

Geissler, Edwin N., Ryan, Melanie A., Housman, David E.·1988·Cell

Abstract

Mutations at the W locus in the mouse have pleiotropic effects on embryonic development and hematopoiesis. The characteristic phenotype of mutants at this locus, which includes white coat color, sterility, and anemia, can be attributed to the failure of stem cell populations to migrate and/or proliferate effectively during development. Mapping experiments suggest that the c-kit proto-oncogene, which encodes a putative tyrosine kinase receptor, is a candidate for the W locus. We show here that the c-kit gene is disrupted in two spontaneous mutant W alleles, W44 and Wx. Genomic DNA that encodes amino acids 240 to 342 of the c-kit polypeptide is disrupted in W44; the region encoding amino acids 342 to 791 is disrupted in Wx. W44 homozygotes exhibit a marked reduction in levels of c-kit mRNA. These results strongly support the identification of c-kit as the gene product of the W locus.

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Crossref · 10.1016/0092-8674(88)90020-7

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Authors
Geissler, E. N., Ryan, M. A., & Housman, D. E.
Affiliations
Massachusetts Institute of Technology (US)
Venue
Cell
Publisher
Elsevier BV
Published
1988-10
Volume
55
Issue
1
Pages
185-192
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2112811492
Cited by
1,287
References
33
Citations by year
2026: 5 · 2025: 6 · 2024: 8 · 2023: 6 · 2022: 9 · 2021: 6 · 2020: 10 · 2019: 11 · 2018: 8 · 2017: 9 · 2016: 11 · 2015: 17 · 2014: 20 · 2013: 24 · 2012: 22
FWCI
43.74
Type
journal article
Language
en
Open access
closed
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Cancer Institute (CA-17575, CA-07826)
  • National Institute of General Medical Sciences (GM 27882)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
Keywords
c-kit proto-oncogene, mouse coat color, receptor tyrosine kinase, gene mapping, embryonic development, hematopoiesis, stem cell migration, anemia, sterility, spontaneous mutation
MeSH terms
Alleles, Animals, Base Sequence, Chromosome Mapping, Embryonic and Fetal Development, Hematopoiesis, Mutation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogenes, Receptors, Cell Surface, RNA, Messenger, Proto-Oncogene Proteins c-kit, Mice

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