The DOI matches its Crossref record; 61 of the 76 references it cites were found in a registry or an index.
The zinc finger transcription factor Egr-1 is essential for and restricts differentiation along the macrophage lineage
Open accessAbstract
We have isolated cDNA clones of myeloid differentiation primary response (MyD) genes, activated in the absence of de novo protein synthesis following induction for differentiation along either the macrophage or granulocyte lineage in human myeloblastic leukemia HL-60 cells. One cDNA clone of a primary response gene, expressed upon macrophage differentiation, encoded for Egr-1, a zinc finger transcription factor. The Egr-1 gene was observed to be transcriptionally silent in HL-60 cells, but active in U-937 and M1 cells, the latter two being predetermined for macrophage differentiation. Egr-1 antisense oligomers in the culture media blocked macrophage differentiation in both myeloid leukemia cell lines and normal myeloblasts. HL-60 cells constitutively expressing an Egr-1 transgene (HL-60Egr-1) could be induced for macrophage, but not granulocyte, differentiation. These observations indicate that expression of Egr-1 is essential for and restricts differentiation of myeloblasts along the macrophage lineage.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It is interesting to note that ICSBP induces a zinc finger transcription factor Egr-1 that has been implicated in macrophage differentiation, (57)”
From Review: ICSBP/IRF-8: Its Regulatory Roles in the Development of Myeloid Cells (2002) · cites this paper as [57]
In the citing paper
“It is interesting to note that ICSBP induces a zinc finger transcription factor Egr-1 that has been implicated in macrophage differentiation, (57)”
From Review: ICSBP/IRF-8: Its Regulatory Roles in the Development of Myeloid Cells (2002) · cites this paper as [57]
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A simple and very efficient method for generating cDNA libraries
Gubler, U., & Hoffman, B. J. (1983) · Gene · doi:10.1016/0378-1119(83)90230-5 · Cited by 4,461
Schreiber, E. H., Matthias, P., Müller, M. M., & Schaffner, W. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.15.6419 · Cited by 4,179
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. E. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,198
Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)
Sundström, C., & Nilsson, K. (1976) · International Journal of Cancer · doi:10.1002/ijc.2910170504 · Cited by 2,409
THE GROWTH OF MOUSE BONE MARROW CELLS IN VITRO
Bradley, T., & Metcalf, D. (1966) · Immunology and Cell Biology · doi:10.1038/icb.1966.28 · Cited by 1,938
The Human Hematopoietic Colony-Stimulating Factors
Clark, S., & Kamen, R. (1987) · Science · doi:10.1126/science.3296190 · Cited by 1,630
Collins, S. J., Ruscetti, F. W., Gallagher, R. E., & Gallo, R. C. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.5.2458 · Cited by 1,594
Sukhatme, V. P., Cao, X., Chang, L. C., Tsai‐Morris, C., Stamenkovich, D., Ferreira, P. C. P., Cohén, D., Edwards, S. A., Shows, T. B., Curran, T., et al. (1988) · Cell · doi:10.1016/0092-8674(88)90485-0 · Cited by 1,249
A Nerve Growth Factor-Induced Gene Encodes a Possible Transcriptional Regulatory Factor
Milbrandt, J. (1987) · Science · doi:10.1126/science.3672127 · Cited by 1,202
Research works printed but not linked: 1
- Selvakumaran, 1992, Deregulated c-myb disrupts IL6/LIF induced myeloid differentiation prior to c-myc: role in leukemogenesis, Mol. Cell. Biol., 12, 2493
Cited by
Sorted by citations
Items 301–367 of 367
Stoddart, A., Fernald, A. A., Davis, E. M., McNerney, M. E., & Beau, M. M. L. (2022) · Experimental Hematology · doi:10.1016/j.exphem.2022.08.003 · Cited by 11
Phospholipase D2 (PLD2) Shortens the Time Required for Myeloid Leukemic Cell Differentiation
Fulvio, M. D., Frondorf, K. A., Henkels, K. M., Grunwald, W. C., Cool, D. R., & Gómez-Cambronero, J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.259465 · Cited by 12
Jones, J. E., Wang, L., Kropf, P. L., Duan, R., & Johnson, D. E. (2009) · Leukemia · doi:10.1038/leu.2009.118 · Cited by 10
Ducruet, A. F., Sosunov, S. A., Visovatti, S., Petrovic‐Djergovic, D., Mack, W. J., Connolly, E. S., & Pinsky, D. J. (2011) · Neurological Research · doi:10.1179/1743132810y.0000000022 · Cited by 10
Loss of Egr1, a human del5q gene, accelerates BCR-ABL driven chronic myelogenous leukemia
Maifrede, S., Magimaidas, A., Sha, X., Mukherjee, K., Liebermann, D. A., & Hoffman‐Liebermann, B. (2017) · Oncotarget · doi:10.18632/oncotarget.20612 · Cited by 10
Self-citationArimochi, H., & Morita, K. (2005) · Journal of Molecular Neuroscience · doi:10.1385/jmn:27:3:293 · Cited by 10
Involvement of Egr-1 in lens epithelial cell death induced by selenite
Nakajima, T., Belusko, P. B., Walkup, R. D., Azuma, M., & Shearer, T. R. (2006) · Experimental Eye Research · doi:10.1016/j.exer.2005.10.020 · Cited by 10
Polimeni, M., Giorgi, S., Gregorio, L. D., Dragani, T. A., Molinaro, M., Cossu, G., & Bouché, M. (1996) · Mechanisms of Development · doi:10.1016/0925-4773(95)00465-3 · Cited by 10
Hypoxia-induced EGR1 remodels neutrophils to suppress antitumor immunity
Chang, T., Friedman, J., Clavijo, P. E., Robbins, Y. L., Craveiro, M., Huynh, A. P., Dar, M. S., Yang, X., Cao, Y., Sievers, C. M., et al. (2025) · Science Immunology · doi:10.1126/sciimmunol.adz2273 · Cited by 9
Dobbin, E., Graham, C., Freeburn, R. W., Unwin, R. D., Griffiths, J. R., Pierce, A., Whetton, A. D., & Wheadon, H. (2010) · Stem Cell Research · doi:10.1016/j.scr.2010.08.001 · Cited by 9
PTEN is indispensable for cells to respond to MAPK inhibitors in myeloid leukemia
Zhang, J., Xiang, Z., Malaviarachchi, P. A., Yan, Y., Baltz, N. J., Emanuel, P. D., & Liu, Y. L. (2018) · Cellular Signalling · doi:10.1016/j.cellsig.2018.06.004 · Cited by 9
How to Establish Acute Myeloid Leukemia Xenograft Models Using Immunodeficient Mice
Shan, W., & Ma, X. (2013) · Asian Pacific Journal of Cancer Prevention · doi:10.7314/apjcp.2013.14.12.7057 · Cited by 9
Li, B., Berman, J. N., Wu, P., Liu, F., Tang, J., & Lin, T. (2008) · Journal of Immunotoxicology · doi:10.1080/15476910802129612 · Cited by 9
GTPase deficient mutant of Gα13 regulates the expression of Egr-1 through the small GTPase Rho
Prasad, M. V., & Dhanasekaran, N. (1999) · Oncogene · doi:10.1038/sj.onc.1202461 · Cited by 9
Differential expression of novel genes by bone marrow-derived macrophage populations
Yang, S., Schook, L. B., & Rutherford, M. S. (1995) · Molecular Immunology · doi:10.1016/0161-5890(95)00035-d · Cited by 9
EGR2 is an epigenomic regulator of phagocytosis and antifungal immunity in alveolar macrophages
Kolostyák, Z., Bojcsuk, D., Baksa, V., Szigeti, Z. M., Bene, K., Czimmerer, Z., Botó, P., Fadel, L., Póliska, S., Halász, L., et al. (2024) · JCI Insight · doi:10.1172/jci.insight.164009 · Cited by 8
Zou, Y., Kamada, N., Seong, S., & Seo, S. (2023) · Communications Biology · doi:10.1038/s42003-023-04650-3 · Cited by 9
An Efficient Strategy to Identify Early TPA-Responsive Genes During Differentiation of HL-60 Cells
Hu, L.-Y., Tepper, C. G., Lo, S. H., & Lin, W. (2006) · Gene Expression · doi:10.3727/000000006783991791 · Cited by 8
Inhibition of Hedgehog signaling induces monocytic differentiation of HL-60 cells
Bai, L., Weng, J., Lo, W.-J., Yeh, S., Wu, C.-Y., Wang, C.-Y., Chiu, C., & Lin, C. (2011) · Leukemia & lymphoma/Leukemia and lymphoma · doi:10.3109/10428194.2011.639877 · Cited by 8
Genetic background-dependent role of Egr1 for eyelid development
Oh, J., Wang, Y., Chen, S., Li, P., Du, N., Yu, Z., Butcher, D. O., Gebregiorgis, T., Strachan, E. R., Lehmann, O. J., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1705848114 · Cited by 8
Schulze, C., Büchse, T., Mikkat, S., & Bittorf, T. (2008) · Cellular Signalling · doi:10.1016/j.cellsig.2008.06.013 · Cited by 7
Radiation-inducible gene therapy
Mauceri, H. J., Hanna, N. N., Staba, M.-J., Beckett, M. A., Kufe, D. W., & Weichselbaum, R. R. (1999) · Comptes Rendus de l Académie des Sciences - Series III - Sciences de la Vie · doi:10.1016/s0764-4469(99)80047-x · Cited by 7
Schütz, B., & Niessing, J. (1994) · Gene · doi:10.1016/0378-1119(94)90693-9 · Cited by 7
Her, S., Arimochi, H., & Morita, K. (2004) · Molecular Brain Research · doi:10.1016/j.molbrainres.2004.04.002 · Cited by 6
Kim, K., Choi, Y. H., Kim, H.-H., & Cheong, J. (2009) · Experimental and Molecular Medicine · doi:10.3858/emm.2009.41.6.048 · Cited by 6
Li, C., Dh, M., & COLEMAN, D. L. (1994) · PubMed Central · Cited by 6
Chauhan, D., Kharbanda, S. M., Uchiyama, H., Urashima, M., & Anderson, K. C. (1995) · Leukemia Research · doi:10.1016/0145-2126(94)00132-t · Cited by 6
Test of Significant Differences with a priori Probability in Microarray Experiments
Toda, K., Ishida, S., Nakata, K., Matsuda, R., Shigemoto‐Mogami, Y., Fujishita, K., Ozawa, S., Sawada, J.-I., Inoue, K., Shudo, K., et al. (2003) · Analytical Sciences · doi:10.2116/analsci.19.1529 · Cited by 6
Ponton, A., Thirion, J., & Sirois, P. (1996) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(96)90088-5 · Cited by 6
Effect of Zinc Upon Human and Murine Cell Viability and Differentiation
Vega-Robledo, G. B., Polo-Jiménez, A., Morales-Martínez, M. E., Rojas‐Dotor, S., & Rico-Rosillo, G. (2007) · Biological Trace Element Research · doi:10.1007/s12011-007-8010-x · Cited by 5
Miyake, N., Katoh, O., Hirata, S., Kimura, S., Watanabe, H., & Yajin, K. (2002) · Cancer Letters · doi:10.1016/s0304-3835(02)00303-8 · Cited by 5
Low EGR1 expression predicts poor prognosis in clear cell renal cell carcinoma
Zhang, Z., Zhang, S., Chen, H., Mao, Y., Kong, C., Li, Z., Wang, L., Ma, M., & Han, B. (2021) · Pathology - Research and Practice · doi:10.1016/j.prp.2021.153666 · Cited by 5
Yang, H. J., Lee, J., Noh, J. K., Kim, H. C., Park, C., Park, J.-W., & Kim, K. (2014) · Development & Reproduction · doi:10.12717/dr.2014.18.4.233 · Cited by 5
mt‐Ty 5' tiRNA regulates skeletal muscle cell proliferation and differentiation
Cao, J., Wang, X., Advani, V. M., Lu, Y. W., Malizia, A. P., Singh, G. B., Huang, Z., Liu, J., Wang, C., de Oliveira, E. M., et al. (2023) · Cell Proliferation · doi:10.1111/cpr.13416 · Cited by 4
Overexpression of the c-fos gene perturbs functional maturation of M1 cells into macrophages
Obata, S., Okada, S., Ohkubo, Y., Murasawa, M., Hatano, M., Sekiya, S., & Tokuhisa, T. (2002) · Molecular Immunology · doi:10.1016/s0161-5890(02)00199-2 · Cited by 5
Control by thyroid hormone of NGFI-A gene expression in lung: regulation of NGFI-A promoter activity
Rodrı́guez-Manzaneque, J. C., Pérez‐Castillo, A., & Santos, Á. (1998) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(98)00089-6 · Cited by 5
Stoyanova, E., Tesch, A., Armstrong, V. W., & Wieland, E. (2001) · Clinical Biochemistry · doi:10.1016/s0009-9120(01)00258-2 · Cited by 5
Mareni, C., Sessarego, M., Montera, M. P., Fugazza, G., Origone, P., D’Amato, E., Lerza, R., Pistoia, V., & Bianchi‐Scarrà, G. (1994) · Leukemia & lymphoma/Leukemia and lymphoma · doi:10.3109/10428199409051688 · Cited by 5
Transcription Factors That Regulate Macrophage Development and Function
Hume, D., & Himes, S. R. (2003) · Handbook of experimental pharmacology · doi:10.1007/978-3-642-55742-2_2 · Cited by 5
Molecular Events in Thymocyte Apoptosis
Smith, S. W., McLaughlin, K. A., & Osborne, B. A. (1995) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-79437-7_11 · Cited by 4
Fu, Q., Wang, X., Zhao, S., Wang, C., Sun, R., Wang, H., Du, P., Wang, J., Wang, X., & Jiang, G. (2022) · Oncology Letters · doi:10.3892/ol.2022.13393 · Cited by 3
Amplified nitric oxide production by pulmonary alveolar macrophages of newborn rats
Sherman, M. P., Wong, V., Aeberhard, E. E., Fukuto, J. M., & Ignarro, L. J. (1996) · Redox Report · doi:10.1080/13510002.1996.11747066 · Cited by 3
Kukita, T. (1997) · Endocrinology · doi:10.1210/en.138.10.4384 · Cited by 3
Skerka, C., Abel, W. O., & Zipfel, P. F. (2000) · Experimental and Clinical Immunogenetics · doi:10.1159/000019122 · Cited by 2
Thomson, A. M., Roberts, N. A., Stanley, J. A. S., Souyri, M., & Wood, W. G. (1999) · British Journal of Haematology · doi:10.1046/j.1365-2141.1999.01688.x · Cited by 2
Bashir, H. S., Binod, G. C., Rahman, A. A., & Dong, F. (2025) · Journal of Leukocyte Biology · doi:10.1093/jleuko/qiaf159 · Cited by 1
Kumaresan, V., Pahari, S., Hung, C.-Y., Hermann, B. P., Schlesinger, L. S., & Seshu, J. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1766756 · Cited by 1
Orthopaedic Wear Particle-Induced Activation of Macrophages
Beidelschies, M. A. (2009) · OhioLink ETD Center (Ohio Library and Information Network) · Cited by 1
Chromosomal Translocations in B-Cell Leukemias and Lymphomas
Look, A. T., & Ferrando, A. A. (2004) · Elsevier eBooks · doi:10.1016/b978-012053641-2/50024-1 · Cited by 1
Improvement in Reliability of Probabilistic Test of Significant Differences in GeneChip Experiments
Toda, K., Ishida, S., Nakata, K., Matsuda, R., Shigemoto‐Mogami, Y., Ozawa, S., Sawada, J.-I., Ohno, Y., Inoue, K., Shudo, K., et al. (2004) · Analytical Sciences · doi:10.2116/analsci.20.731 · Cited by 1
Gene expression profile of Jurkat cells exposed to high power terahertz radiation
Grundt, J. E., Roth, C. C., Rivest, B. D., Doroski, M. L., Payne, J., Ibey, B. L., & Wilmink, G. J. (2011) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.874255 · Cited by 1
The Role of Stat1 in Retinoic Acid-Induced Myelomonocytic Differentiation of Human Leukemia Cells
Dimberg, A. (2002) · KTH Publication Database DiVA (KTH Royal Institute of Technology) · Cited by 1
The Zebrafish egrl Gene Encodes a Highly Conserved, Zinc-Finger Transcriptional Regulator
Drummond, I. A. S., Rohwer-Nutter, P., & Sukhatme, V. P. (1994) · DNA and Cell Biology · doi:10.1089/dna.1994.13.953 · Cited by 1
Li, C., Mitchell, D. H., & COLEMAN, D. L. (1995) · Journal of Endotoxin Research · doi:10.1177/096805199500200403
Close, R., Toro, S., MARTIAL, J. A., & Müller, M. (2002)
Cortés, C., & Júlio, R. L. (2013) · Vertientes. Revista Especializada en Ciencias de la Salud
Der Transkriptionsfaktor Egr-1 reguliert das Wachstum glomerulärer Mesangiumzellen
Rupprecht, H., Höfer, G., Sterzel, R. B., & Schöcklmann, H. O. (1997) · Medizinische Klinik - Intensivmedizin und Notfallmedizin · doi:10.1007/bf03042287
EGR-1 tumor suppressor in BCR-ABL driven leukemia
Maifrede, S. (2015) · TUScholarShare (Temple University)
Dando, C. (1997) · Enlighten: Theses (The University of Glasgow)
Antisense Strategies to Leukemia
O’Brien, S. G. (1999) · Blood cell biochemistry · doi:10.1007/978-1-4615-4889-8_10
Inference of marker genes of subtle cell state changes via iLR: iterative logistic regression
Liu, Y., Baugh, A. G., Torres, E. T. R., & MacLean, A. L. (2026) · Bioinformatics · doi:10.1093/bioinformatics/btag051 · Cited by 2
Terret-Guéry, L. (2010) · theses.fr (ABES) · doi:10.70675/005598efzec21z43f2zb22ez300245496e37
Rôle de miR-142-3p dans la régulation de la différenciation macrophagique
Lagrange, B. (2011) · theses.fr (ABES) · doi:10.70675/b47d3b61zc6f3z45d0zbc15zf8c5229a738f
Genetic Anatomy and Ontogenetic Roles of Early Growth Response 1 (Egr1) in Human and Mouse
Na, I.-H., & Cheon, Y. (2026) · Development & Reproduction · doi:10.12717/dr.2026.30.1.1
The Control of Multiplication and Differentiation in Human Myelomonocytic Leukemia Cells
Collart, F., Tonetti, D. A., & Huberman, E. (1996) · Springer eBooks · doi:10.1007/978-3-642-61180-3_11
Metadata
- Authors
- Nguyen, H. Q., Hoffman-Liebermann, B., & Liebermann, D. A.
- Affiliations
- University of Pennsylvania (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1993-01
- Volume
- 72
- Issue
- 2
- Pages
- 197-209
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1993004730
- Cited by
- 367
- References
- 76
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 7 · 2018: 4 · 2017: 7 · 2016: 1 · 2015: 5 · 2014: 6 · 2013: 7 · 2012: 7
- FWCI
- 21.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ca043618-12)
- National Cancer Institute (1RO1CA43618-01)
- Topics
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- EGR1, zinc finger transcription factor, macrophage differentiation, granulocytic differentiation, HL-60 cells, U937 cells, M1 cells, antisense oligomers, transgene expression, myeloblasts, transcriptional regulation
- MeSH terms
- Animals, Base Sequence, Binding Sites, Bone Marrow, Bone Marrow Cells, Cell Differentiation, Cell Nucleus, Dimethyl Sulfoxide, DNA-Binding Proteins, DNA, Neoplasm, Granulocytes, Hematopoietic Stem Cells, Humans, Macrophages, Mice, Inbred Strains, Molecular Sequence Data, Poly A, RNA, RNA, Messenger, Tetradecanoylphorbol Acetate, Transcription Factors, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Leukemia, Promyelocytic, Acute, Gene Expression, Zinc Fingers, Oligonucleotides, Antisense, Immediate-Early Proteins, Mice, Early Growth Response Protein 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review