Cooperative activation of muscle gene expression by MEF2 and myogenic bHLH proteins
Open accessAbstract
Members of the myocyte enhancer factor-2 (MEF2) family of MADS domain transcription factors cannot induce myogenesis in transfected fibroblasts, but when coexpressed with the myogenic basic-helix-loop-helix (bHLH) proteins MyoD or myogenin they dramatically increase the extent of myogenic conversion above that seen with either myogenic bHLH factor alone. This cooperativity required direct interactions between the DNA-binding domains of MEF2 and the myogenic bHLH factors, but only one of the factors needed a transactivation domain, and only one of the factors needed to be bound to DNA. These interactions allow either factor to activate transcription through the other's binding site and reveal a novel mechanism for indirect activation of gene expression via protein-protein interactions between the DNA-binding domains of heterologous classes of transcription factors.
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Across Bilateria, cell type-specific gene expression in striated muscle cells is controlled by members of the MyoD family of bHLH transcriptional regulators and by regulators of the Mef2 class.
“Across the Bilateria, for example, cell type-specific gene expression in striated muscle cells is controlled by members of the MyoD family of bHLH transcriptional regulators and by regulators of the Mef2 class (Molkentin et al., 1995) (reviewed by Zhang et al., 1999).”
From The last common bilaterian ancestor (2002) · cites this paper as Molkentin et al., 1995
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- Authors
- Molkentin, J. D., Black, B. L., Martin, J. F., & Olson, E. N.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 83
- Issue
- 7
- Pages
- 1125-1136
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2106468261
- Cited by
- 819
- References
- 1
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 5 · 2023: 12 · 2022: 19 · 2021: 20 · 2020: 15 · 2019: 8 · 2018: 15 · 2017: 22 · 2016: 32 · 2015: 25 · 2014: 19 · 2013: 24 · 2012: 19
- FWCI
- 18.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- MEF2, MyoD, myogenin, muscle gene expression, myogenesis, transcriptional activation, protein-protein interaction, DNA-binding domain, transactivation domain, MADS-box transcription factors
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, DNA-Binding Proteins, Embryonic and Fetal Development, Fibroblasts, Molecular Sequence Data, Muscles, Transcription Factors, Transcription, Genetic, Gene Deletion, Point Mutation, MyoD Protein, Myogenic Regulatory Factors, Myogenin, Helix-Loop-Helix Motifs, Gene Expression Regulation, Developmental, Mice, Basic Helix-Loop-Helix Proteins, MEF2 Transcription Factors
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