l ‐glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy After Cytokine (TNF‐α) Stress Via Reduced p38 MAPK Signal Transduction
Open accessAbstract
Tumour Necrosis Factor-Alpha (TNF-α) is chronically elevated in conditions where skeletal muscle loss occurs. As l-glutamine can dampen the effects of inflamed environments, we investigated the role of l-glutamine in both differentiating C2C12 myoblasts and existing myotubes in the absence/presence of TNF-α (20 ng · ml(-1) ) ± l-glutamine (20 mM). TNF-α reduced the proportion of cells in G1 phase, as well as biochemical (CK activity) and morphological differentiation (myotube number), with corresponding reductions in transcript expression of: Myogenin, Igf-I, and Igfbp5. Furthermore, when administered to mature myotubes, TNF-α induced myotube loss and atrophy underpinned by reductions in Myogenin, Igf-I, Igfbp2, and glutamine synthetase and parallel increases in Fox03, Cfos, p53, and Bid gene expression. Investigation of signaling activity suggested that Akt and ERK1/2 were unchanged, JNK increased (non-significantly) whereas P38 MAPK substantially and significantly increased in both myoblasts and myotubes in the presence of TNF-α. Importantly, 20 mM l-glutamine reduced p38 MAPK activity in TNF-α conditions back to control levels, with a corresponding rescue of myoblast differentiation and a reversal of atrophy in myotubes. l-glutamine resulted in upregulation of genes associated with growth and survival including; Myogenin, Igf-Ir, Myhc2 & 7, Tnfsfr1b, Adra1d, and restored atrophic gene expression of Fox03 back to baseline in TNF-α conditions. In conclusion, l-glutamine supplementation rescued suppressed muscle cell differentiation and prevented myotube atrophy in an inflamed environment via regulation of p38 MAPK. l-glutamine administration could represent an important therapeutic strategy for reducing muscle loss in catabolic diseases and inflamed ageing. J. Cell. Physiol. 9999: 231: 2720-2732, 2016. © 2016 Wiley Periodicals, Inc.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
l-glutamine improves C2C12 cell differentiation and prevents TNF-α-induced myotube atrophy via reducing p38 MAPK signal transduction.
“l-glutamine improves C2C12 cells differentiation and prevents TNF-α-induced myotube atrophy via reducing p38 mitogen-activated protein kinase (MAPK) signal transduction [35].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [35]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Analyzing real-time PCR data by the comparative CT method
Schmittgen, T. D., & Livak, K. J. (2008) · Nature Protocols · doi:10.1038/nprot.2008.73 · Cited by 26,190 · see citing works
Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase
Brunet, A., Sweeney, L. B., Sturgill, J. F., Chua, K. F., Greer, P. L., Lin, Y., Tran, H., Ross, S. E., Mostoslavsky, R., Cohen, H. Y., et al. (2004) · Science · doi:10.1126/science.1094637 · Cited by 3,239 · see citing works
Sandri, M., Sandri, C., Gilbert, A., Skurk, C., Calabria, E., Picard, A., Walsh, K., Schiaffino, S., Lecker, S. H., & Goldberg, A. L. (2004) · Cell · doi:10.1016/s0092-8674(04)00400-3 · Cited by 3,012 · see citing works
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle
Yaffe, D., & Saxel, O. (1977) · Nature · doi:10.1038/270725a0 · Cited by 2,153 · see citing works
FOXO transcription factors at the interface between longevity and tumor suppression
Greer, E. L., & Brunet, A. (2005) · Oncogene · doi:10.1038/sj.onc.1209086 · Cited by 1,306 · see citing works
Interleukin-6 Is an Essential Regulator of Satellite Cell-Mediated Skeletal Muscle Hypertrophy
Serrano, A. L., Baeza-Raja, B., Perdiguero, E., Jardí, M., & Muñoz-Cánoves, P. (2008) · Cell Metabolism · doi:10.1016/j.cmet.2007.11.011 · Cited by 873 · cited 1× · see citing works
“Ourselves and others have demonstrated early acute increases in TNF-a are important in skeletal muscle cell proliferation via activation of the MAPK’s (Li, 2003; Foulstone et al., 2004; Stewart et al., 2004; Al-Shanti et al., 2008; Serrano et al., 2008; Sharples et al., 2010).”
MyoD and the transcriptional control of myogenesis
Berkes, C. A., & Tapscott, S. J. (2005) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2005.07.006 · Cited by 848 · see citing works
Plasticity of the Differentiated State
Blau, H. M., Pavlath, G. K., Hardeman, E. C., Chiu, C., Silberstein, L., Webster, S. G., Miller, S. C., & Webster, C. (1985) · Science · doi:10.1126/science.2414846 · Cited by 840 · see citing works
Signaling in Muscle Atrophy and Hypertrophy
Sandri, M. (2008) · Physiology · doi:10.1152/physiol.00041.2007 · Cited by 830 · see citing works
Cooperative activation of muscle gene expression by MEF2 and myogenic bHLH proteins
Molkentin, J. D., Black, B. L., Martin, J. F., & Olson, E. N. (1995) · Cell · doi:10.1016/0092-8674(95)90139-6 · Cited by 819 · see citing works
Is Glutamine a Conditionally Essential Amino Acid?
Lacey, J. M., & Wilmore, D. W. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.1990.tb02967.x · Cited by 808 · see citing works
SIRT2 deacetylates FOXO3a in response to oxidative stress and caloric restriction
Wang, F., Nguyen, M., Qin, F. X.-F., & Tong, Q. (2007) · Aging Cell · doi:10.1111/j.1474-9726.2007.00304.x · Cited by 652 · see citing works
Coolican, S. A., Samuel, D. S., Ewton, D. Z., McWade, F. J., & Florini, J. R. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.10.6653 · Cited by 646 · see citing works
Regulation of Glutaminase Activity and Glutamine Metabolism
Curthoys, N. P., & Watford, M. (1995) · Annual Review of Nutrition · doi:10.1146/annurev.nu.15.070195.001025 · Cited by 643 · see citing works
Age-related inflammatory cytokines and disease
Brüünsgaard, H., & Pedersen, B. K. (2003) · Immunology and Allergy Clinics of North America · doi:10.1016/s0889-8561(02)00056-5 · Cited by 608 · see citing works
Li, Y.-P., Chen, Y., John, J., Moylan, J. S., Jin, B., Mann, D. L., & Reid, M. B. (2005) · The FASEB Journal · doi:10.1096/fj.04-2364com · Cited by 601 · see citing works
Resistance exercise decreases skeletal muscle tumor necrosis factor α in frail elderly humans
GREIWE, J. S., CHENG, B., RUBIN, D. C., YARASHESKI, K. E., & SEMENKOVICH, C. F. (2001) · The FASEB Journal · doi:10.1096/fj.00-0274com · Cited by 454 · see citing works
NF-κB mediates the protein loss induced by TNF-α in differentiated skeletal muscle myotubes
Li, Y., & Reid, M. B. (2000) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2000.279.4.r1165 · Cited by 443 · see citing works
Palacios, D., Mozzetta, C., Consalvi, S., Caretti, G., Saccone, V., Proserpio, V., Marquez, V. E., Valente, S., Mai, A., Forcales, S. V., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.013 · Cited by 392 · see citing works
The p38 MAPK signaling pathway: A major regulator of skeletal muscle development
Keren, A., Tamir, Y., & Bengal, E. (2006) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2006.03.017 · Cited by 372 · see citing works
Does glutamine contribute to immunosuppression after major burns?
Parry‐Billings, M., Calder, P. C., Newsholme, E. A., & Evans, J. (1990) · The Lancet · doi:10.1016/0140-6736(90)92083-t · Cited by 352 · see citing works
Metabolic disorders in severe abdominal sepsis: Glutamine deficiency in skeletal muscle
Roth, E., Funovics, M. A., Mühlbacher, F., SCHEMPER, M., Mauritz, W., Sporn, P., & Fritsch, A. (1982) · Clinical Nutrition · doi:10.1016/0261-5614(82)90004-8 · Cited by 335 · see citing works
Elevated levels of tumor necrosis factor alpha and mortality in centenarians
Bruunsgaard, H., Andersen-Ranberg, K., Hjelmborg, J. V. B., Pedersen, B. K., & Jeune, B. (2003) · The American Journal of Medicine · doi:10.1016/s0002-9343(03)00329-2 · Cited by 325 · see citing works
May, P. E., Barber, A. E., D'Olimpio, J. T., Hourihane, A., & Abumrad, N. N. (2002) · The American Journal of Surgery · doi:10.1016/s0002-9610(02)00823-1 · Cited by 318 · see citing works
TNF-α regulates myogenesis and muscle regeneration by activating p38 MAPK
Chen, S., Jin, B., & Li, Y.-P. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00486.2006 · Cited by 301 · see citing works
Predicting death from tumour necrosis factor-alpha and interleukin-6 in 80-year-old people
BRUUNSGAARD, H., LADELUND, S., PEDERSEN, A. N., SCHROLL, M., JØRGENSEN, T., & PEDERSEN, B. K. (2003) · Clinical and Experimental Immunology · doi:10.1046/j.1365-2249.2003.02137.x · Cited by 300 · see citing works
Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch
Yang, S., Alnaqeeb, M. A., Simpson, H., & Goldspink, G. (1996) · Journal of Muscle Research and Cell Motility · doi:10.1007/bf00123364 · Cited by 296 · see citing works
Léger, B., Derave, W., Bock, K. D., Hespel, P., & Russell, A. P. (2008) · Rejuvenation Research · doi:10.1089/rej.2007.0588 · Cited by 274 · see citing works
Tumor Necrosis Factor: Function, Release and Clearance
Bemelmans, M. H. A., van Tits, L. J. H., & Buurman, W. A. (1996) · Critical Reviews in Immunology · doi:10.1615/critrevimmunol.v16.i1.10 · Cited by 274 · see citing works
TNF-α is a mitogen in skeletal muscle
Li, Y.-P. (2003) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00453.2002 · Cited by 249 · see citing works
TNF‐α increases ubiquitin‐conjugating activity in skeletal muscle by up‐regulating UbcH2/E2 20k
LI, Y.-P., Lecker, S. H., Chen, Y., Waddell, I. D., Goldberg, A. L., & Reid, M. B. (2003) · The FASEB Journal · doi:10.1096/fj.02-0759com · Cited by 243 · see citing works
Glutamine and the immune system
Calder, P. C., & Yaqoob, P. (1999) · Amino Acids · doi:10.1007/bf01366922 · Cited by 204 · see citing works
Sharples, A. P., Hughes, D. C., Deane, C. S., Saini, A. S., Selman, C., & Stewart, C. E. (2015) · Aging Cell · doi:10.1111/acel.12342 · Cited by 200 · see citing works
Self-citationMackey, A. L., Kjær, M., Dandanell, S., Mikkelsen, S. U., Holm, L., Døssing, S., Kadi, F., Koskinen, S. O. A., Jensen, C. H., Schrøder, H. D., et al. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00157.2007 · Cited by 198 · see citing works
E47 phosphorylation by p38 MAPK promotes MyoD/E47 association and muscle‐specific gene transcription
Lluı́s, F., Ballestar, E., Suelves, M., Esteller, M., & Muñoz‐Cánoves, P. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600528 · Cited by 197 · see citing works
Atrogin-1/MAFbx and MuRF1 are downregulated in aging-related loss of skeletal muscle
Edstrom, E., Altun, M., Hagglund, M., & Ulfhake, B. (2006) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/61.7.663 · Cited by 196 · see citing works
Cytokines and oxidative signalling in skeletal muscle
Reid, M. B., & Li, Y. ‐. (2001) · Acta Physiologica Scandinavica · doi:10.1046/j.1365-201x.2001.00824.x · Cited by 187 · see citing works
Mikkelsen, U. R., Langberg, H., Helmark, I. C., Skovgaard, D., Andersen, L. L., Kjær, M., & Mackey, A. L. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00707.2009 · Cited by 185 · see citing works
Tollefsen, S. E., Sadow, J. L., & Rotwein, P. S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.5.1543 · Cited by 177 · see citing works
Glutamine Metabolism in Sepsis and Infection
Karinch, A. M., Pan, M., Lin, C., Strange, R., & Souba, W. W. (2001) · Journal of Nutrition · doi:10.1093/jn/131.9.2535s · Cited by 173 · see citing works
Waste management—Cytokines, growth factors and cachexia
Saini, A., Al‐Shanti, N., & Stewart, C. E. (2006) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2006.09.006 · Cited by 165 · see citing works
Self-citationLabow, B. I., & Souba, W. W. (2000) · World Journal of Surgery · doi:10.1007/s002680010269 · Cited by 155 · see citing works
Singleton, K. D., Beckey, V. E., & Wischmeyer, P. E. (2005) · Shock · doi:10.1097/01.shk.0000185795.96964.71 · Cited by 154 · see citing works
Regulation of GDF-8 signaling by the p38 MAPK
Philip, B., Lu, Z., & Gao, Y. (2004) · Cellular Signalling · doi:10.1016/j.cellsig.2004.08.003 · Cited by 153 · see citing works
Glutamine and the Immune System
Cruzat, V. F., & Newsholme, P. (2017) · CRC Press eBooks · doi:10.1201/9781315118901-21 · Cited by 153 · see citing works
Clinical Use of Glutamine Supplementation
Wernerman, J. (2008) · Journal of Nutrition · doi:10.1093/jn/138.10.2040s · Cited by 151 · see citing works
Aging increases the susceptibility of skeletal muscle derived satellite cells to apoptosis
Jejurikar, S. S., Henkelman, E., Cederna, P. S., Marcelo, C. L., Urbanchek, M. G., & Kuzon, W. M. (2006) · Experimental Gerontology · doi:10.1016/j.exger.2006.06.053 · Cited by 133 · see citing works
Wischmeyer, P. E., Riehm, J., Singleton, K. D., Ren, H., Musch, M. W., Kahana, M. D., & Chang, E. B. (2002) · Nutrition · doi:10.1016/s0899-9007(02)00839-0 · Cited by 132 · see citing works
P38-γ–dependent gene silencing restricts entry into the myogenic differentiation program
Gillespie, M. A., Le Grand, F., Scimè, A., Kuang, S., von Maltzahn, J., Seale, V., Cuenda, A., Ranish, J. A., & Rudnicki, M. A. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200907037 · Cited by 131 · cited 1× · see citing works
“It has also been demonstrated that a different p38 MAPK family member, p38-g, also plays a crucial role in regulating skeletal muscle differentiation (Gillespie et al., 2009; Lassar, 2009).”
HDAC1 activates FoxO and is both sufficient and required for skeletal muscle atrophy
Beharry, A. W., Sandesara, P. B., Roberts, B. M., Ferreira, L. F., Senf, S. M., & Judge, A. R. (2014) · Journal of Cell Science · doi:10.1242/jcs.136390 · Cited by 128 · see citing works
Li, Y. (2000) · Cellular Signalling · doi:10.1016/s0898-6568(00)00120-0 · Cited by 115 · see citing works
Glutamine and glutamate: Nonessential or essential amino acids?
Watford, M. (2015) · Animal nutrition · doi:10.1016/j.aninu.2015.08.008 · Cited by 112 · see citing works
Mechanisms involved in IL-6-induced muscular mechanical hyperalgesia in mice
Manjavachi, M. N., Motta, E. M., Marotta, D. M., Leite, D. F. P., & Calixto, J. Β. (2010) · Pain · doi:10.1016/j.pain.2010.07.018 · Cited by 106 · see citing works
Multifaceted roles of TNF‐α in myoblast destruction: A multitude of signal transduction pathways
Stewart, C. E., Newcomb, P. V., & Holly, J. M. P. (2003) · Journal of Cellular Physiology · doi:10.1002/jcp.10387 · Cited by 99 · see citing works
Self-citationLessor, T. J., Yoo, J.-Y., Xia, X., Woodford, N., & Hamburger, A. W. (2000) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(200006)183:3 · Cited by 93 · see citing works
TACE release of TNF-α mediates mechanotransduction-induced activation of p38 MAPK and myogenesis
Zhan, M., Jin, B., Chen, S., Reecy, J. M., & Li, Y.-P. (2007) · Journal of Cell Science · doi:10.1242/jcs.03372 · Cited by 89 · see citing works
Foulstone, E. J., Huser, C. A. M., Crown, A. L., Holly, J. M. P., & Stewart, C. E. (2003) · Experimental Cell Research · doi:10.1016/j.yexcr.2003.10.034 · Cited by 74 · see citing works
Self-citationSalehian, B., Mahabadi, V., Bilas, J., Taylor, W. E., & Ma, K. (2006) · Metabolism · doi:10.1016/j.metabol.2006.05.009 · Cited by 69 · see citing works
Characterization of differentiated subcutaneous and visceral adipose tissue from children
Grohmann, M. J., Sabin, M. A., Holly, J. M. P., Hamilton-Shield, J. P., Crowne, E. C., & Stewart, C. E. (2004) · Journal of Lipid Research · doi:10.1194/jlr.m400295-jlr200 · Cited by 68 · see citing works
Self-citationFoulstone, E. J., Savage, P. B., Crown, A. L., et al. (2003) · Journal of Cellular Physiology · doi:10.1002/jcp.10227 · Cited by 67 · see citing works
Sharples, A. P., Polydorou, I., Hughes, D. C., Owens, D. J., Hughes, T. M., & Stewart, C. E. (2015) · Biogerontology · doi:10.1007/s10522-015-9604-x · Cited by 64 · see citing works
Self-citationSharples, A. P., Al‐Shanti, N., & Stewart, C. E. (2010) · Journal of Cellular Physiology · doi:10.1002/jcp.22252 · Cited by 63 · see citing works
Self-citationFoulstone, E. J., Meadows, K. A., Holly, J. M. P., & Stewart, C. E. (2001) · Journal of Cellular Physiology · doi:10.1002/jcp.10017 · Cited by 57 · see citing works
Self-citationBemelmans, M. H. A., Greve, J. M. D., Gouma, D. J., et al. (1996) · Gut · doi:10.1136/gut.38.3.447 · Cited by 54 · see citing works
Tolosa, L., Morlá, M., Iglesias, A., Busquets, X., Lladó, J., & Olmos, G. (2005) · Cellular Signalling · doi:10.1016/j.cellsig.2005.02.001 · Cited by 53 · see citing works
Bozá, J., Turini, M. E., Moënnoz, D., Montigon, F., Vuichoud, J., Gueissaz, N., Gremaud, G., Pouteau, E. B., Piguet-Welsch, C., Finot, P. A., et al. (2001) · Nutrition · doi:10.1016/s0899-9007(00)00505-0 · Cited by 53 · see citing works
Lagranha, C. J., Hirabara, S. M., Curi, R., & Pithon‐Curi, T. C. (2007) · Cell Biochemistry and Function · doi:10.1002/cbf.1421 · Cited by 49 · see citing works
Kim, J., Won, K., Lee, H. M., Hwang, B.-Y., Bae, Y. M., Choi, W. S., Song, H., Lim, K. W., Lee, C., & Kim, B. (2009) · Korean Journal of Physiology and Pharmacology · doi:10.4196/kjpp.2009.13.6.491 · Cited by 48 · see citing works
Al‐Shanti, N., Saini, A. S., Faulkner, S. H., & Stewart, C. E. (2008) · Growth Factors · doi:10.1080/08977190802025024 · Cited by 44 · see citing works
Self-citationSaini, A. S., Al‐Shanti, N., Sharples, A. P., & Stewart, C. E. (2011) · Experimental Physiology · doi:10.1113/expphysiol.2011.061028 · Cited by 44 · see citing works
Self-citationBonetto, A., Penna, F., Minero, V. G., Reffo, P., Costamagna, D., Bonelli, G., Baccino, F. M., & Costelli, P. (2010) · Amino Acids · doi:10.1007/s00726-010-0683-3 · Cited by 43 · see citing works
van de Vyver, M., & Myburgh, K. H. (2012) · Journal of Muscle Research and Cell Motility · doi:10.1007/s10974-012-9303-z · Cited by 43 · see citing works
Sharples, A. P., Al‐Shanti, N., Hughes, D. C., Lewis, M. P., & Stewart, C. E. (2013) · Growth Hormone & IGF Research · doi:10.1016/j.ghir.2013.03.004 · Cited by 42 · see citing works
Self-citationGlutamine Protects Mice from Lethal Endotoxic Shock via a Rapid Induction of MAPK Phosphatase-1
Ko, H., Oh, S., Bang, H.-S., Kang, N.-I., Cho, B., Im, S., & Lee, H.-K. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0900043 · Cited by 38 · see citing works
Alvarez, B., Quinn, L. S., Busquets, S., López-Soriano, F. J., & Argilés, J. M. A. (2001) · PubMed · Cited by 36 · see citing works
Pro- and anti-apoptotic roles for IGF-I in TNF-α-induced apoptosis: A MAP kinase mediated mechanism
Saini, A. S., Al‐Shanti, N., Faulkner, S. H., & Stewart, C. E. (2008) · Growth Factors · doi:10.1080/08977190802291634 · Cited by 36 · see citing works
Self-citationGrohmann, M. J., Foulstone, E. J., Welsh, G. I., Holly, J. M. P., Hamilton-Shield, J. P., Crowne, E. C., & Stewart, C. E. (2005) · The Journal of Physiology · doi:10.1113/jphysiol.2005.093906 · Cited by 28 · see citing works
Self-citationPD98059 enhances C2 myoblast differentiation through p38 MAPK activation: a novel role for PD98059
Al‐Shanti, N., & Stewart, C. E. (2008) · Journal of Endocrinology · doi:10.1677/joe-08-0151 · Cited by 28 · see citing works
Self-citationThe p38 MAPK family, a pushmi-pullyu of skeletal muscle differentiation
Lassar, A. B. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200911123 · Cited by 25 · see citing works
Berg, A., Forsberg, E., & Wernerman, J. (2002) · Clinical Nutrition · doi:10.1054/clnu.2001.0520 · Cited by 25 · see citing works
Schubert, R., Geiger, H., Zielen, S., & Baer, P. C. (2009) · Journal of Immunological Methods · doi:10.1016/j.jim.2009.08.015 · Cited by 21 · see citing works
Stewart, C. E., Newcomb, P. V., Savage, P. B., Dickens, M., Tavaré, J. M., & Holly, J. M. P. (1999) · Growth Hormone & IGF Research · doi:10.1054/ghir.1999.0098 · Cited by 18 · see citing works
Self-citationC2 Skeletal Myoblast Survival, Death, Proliferation and Differentiation: Regulation by Adra1d
Saini, A., Al-Shanti, N., & Stewart, C. (2010) · Cellular Physiology and Biochemistry · doi:10.1159/000276559 · Cited by 12 · see citing works
Self-citationBode, B. P., Fischer, C. P., Abcouwer, S. F., Wasa, M., & Souba, W. W. (1996) · Medical intelligence unit · doi:10.1007/978-3-662-22346-8_11 · Cited by 6 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Labow BI, Souba WW. 2000. Glutamine. World J Surg 24(12):1503-1513.
- Meadows KA, Holly JM, Stewart CE. 2000. Tumor necrosis factor-alpha-induced apoptosis is associated with suppression of insulin-like growth factor binding protein-5 secretion in differentiating murine skeletal myoblasts. J Cell Physiol 183(3):330-337.
Cited by
Sorted by citations
Human skeletal muscle possesses an epigenetic memory of hypertrophy
Seaborne, R. A., Strauss, J., Cocks, M., Shepherd, S., O’Brien, T. D., van Someren, K. A., Bell, P. G., Murgatroyd, C., Morton, J. P., Stewart, C. E., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-20287-3 · Cited by 334 · see citing works
Self-citationInflammation and age-associated skeletal muscle deterioration (sarcopaenia)
Wang, J., Leung, K.-S., Chow, S. K.-H., & Cheung, W.-H. (2017) · Journal of Orthopaedic Translation · doi:10.1016/j.jot.2017.05.006 · Cited by 173 · see citing works
Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy
Wang, Y., Liu, Q., Quan, H., Kang, S.-G., Huang, K., & Tong, T. (2021) · Nutrients · doi:10.3390/nu13061914 · Cited by 61 · see citing works
l-Carnitine Supplementation in Older Women. A Pilot Study on Aging Skeletal Muscle Mass and Function
Sawicka, A., Hartmane, D., Lipinska, P., Wojtowicz, E., Lysiak-Szydlowska, W., & Olek, R. (2018) · Nutrients · doi:10.3390/nu10020255 · Cited by 49 · see citing works
Oral L-Glutamine Pretreatment Attenuates Skeletal Muscle Atrophy Induced by 24-h Fasting in Mice
de Vasconcelos, D. A. A., Giesbertz, P., de Souza, D. R., Vitzel, K. F., Abreu, P., Marzuca-Nassr, G. N., Fortes, M. A. S., Murata, G. M., Hirabara, S. M., Curi, R., et al. (2019) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2019.05.010 · Cited by 33 · see citing works
- Authors
- Girven, M., Dugdale, H. F., Owens, D. J., Hughes, D. C., Stewart, C. E., & Sharples, A. P.
- Affiliations
- Liverpool John Moores University (GB)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Sharples, Adam P.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 2016-04-14
- Volume
- 231
- Issue
- 12
- Pages
- 2720-2732
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2299371824
- Cited by
- 48
- References
- 84
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 4 · 2020: 3 · 2019: 9 · 2018: 5 · 2017: 4 · 2016: 2
- FWCI
- 3.46
- Type
- journal article
- Language
- en
- Open access
- Locations
- Journal of Cellular Physiology (publishedVersion)
- PubMed (publishedVersion)
- Liverpool John Moores University (submittedVersion)
- Funders
- Liverpool John Moores University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Keywords
- glutamine, skeletal muscle atrophy, myotube atrophy, TNF-α, p38 MAPK signaling, myogenesis, C2C12 myoblasts, muscle atrophy, inflammation, FOXO3, myogenin, insulin-like growth factor I, glutamine synthetase, muscle catabolism
- MeSH terms
- Animals, Atrophy, Cell Differentiation, Cell Fusion, Cell Survival, Gene Expression Regulation, Glutamine, Phosphorylation, Stress, Physiological, Tumor Necrosis Factor-alpha, Muscle Fibers, Skeletal, MAP Kinase Signaling System, Muscle Development, Myoblasts, Skeletal, p38 Mitogen-Activated Protein Kinases, Cell Shape, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
