A Basic Helix-Loop-Helix–Leucine Zipper Transcription Complex in Yeast Functions in a Signaling Pathway from Mitochondria to the Nucleus
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The expression of some nuclear genes in Saccharomyces cerevisiae, such as the CIT2 gene, which encodes a glyoxylate cycle isoform of citrate synthase, is responsive to the functional state of mitochondria. Previous studies identified a basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor encoded by the RTG1 gene that is required for both basal expression of the CIT2 gene and its increased expression in respiratory-deficient cells. Here, we describe the cloning and characterization of RTG3, a gene encoding a 54-kDa bHLH/Zip protein that is also required for CIT2 expression. Rtg3p binds together with Rtg1p to two identical sites oriented as inverted repeats 28 bp apart in a regulatory upstream activation sequence element (UASr) in the CIT2 promoter. The core binding site for the Rtg1p-Rtg3p heterodimer is 5'-GGTCAC-3', which differs from the canonical E-box site, CANNTG, to which most other bHLH proteins bind. We demonstrate that both of the Rtg1p-Rtg3p binding sites in the UAS(r) element are required in vivo and act synergistically for CIT2 expression. The basic region of Rtg3p conforms well to the basic region of most bHLH proteins, whereas the basic region of Rtg1p does not. These findings suggest that the Rtg1p-Rtg3p complex interacts in a novel way with its DNA target sites.
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The motif at the 5′ end of this consensus sequence is highly similar to the GTCAC motif (R box) reported to be bound by Saccharomyces cerevisiae Rtg1/Rtg3.
“The motif at the 5′ end of this consensus sequence is highly similar to the ‘GTCAC’ motif (R box) reported to be bound by Saccharomyces cerevisiae Rtg1/Rtg335.”
From Multifactor transcriptional control of alternative oxidase induction integrates diverse environmental inputs to enable fungal virulence (2023) · cites this paper as [35]
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- Authors
- Jia, Y., Rothermel, B., Thornton, J., & Butow, R. A.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Butow, Ronald A.
- ORCID
- Rothermel, Beverly
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1997-03
- Volume
- 17
- Issue
- 3
- Pages
- 1110-1117
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC231836
- OpenAlex
- W2161760772
- Cited by
- 219
- References
- 45
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 1 · 2021: 11 · 2020: 5 · 2019: 5 · 2018: 6 · 2017: 11 · 2016: 7 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 4
- FWCI
- 3.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Welch Foundation
- National Institutes of Health
- National Institute of General Medical Sciences (GM22525)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- bHLH-Zip transcription factors, citrate synthase, glyoxylate cycle, mitochondrial signaling, upstream activating sequence, E-box, promoter binding sites, transcriptional regulation, Saccharomyces cerevisiae
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cell Nucleus, Citrate (si)-Synthase, Cloning, Molecular, DNA-Binding Proteins, DNA, Fungal, DNA, Mitochondrial, Fungal Proteins, Genes, Fungal, Mitochondria, Molecular Sequence Data, Rho Factor, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Signal Transduction, Gene Expression Regulation, Fungal, Leucine Zippers, Sequence Analysis, DNA, Helix-Loop-Helix Motifs, Saccharomyces cerevisiae Proteins, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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