Transfer of Tn1545 and Tn916 to Clostridium acetobutylicum
Abstract
Tn1545, a conjugative transposon originally discovered in Streptococcus pneumoniae, has been transferred from Enterococcus faecalis and Bacillus subtilis to Clostridium acetobutylicum NCIB 8052. Transfer between different strains of C. acetobutylicum has also been observed. Insertion of Tn1545 into the C. acetobutylicum chromosome occurred at multiple sites, as shown by Southern hybridization. Although ermAM (erythromycin-resistance) was the most satisfactory marker for primary selection of transconjugants, all three Tn1545-encoded antibiotic resistance genes (aphA-3, ermAM, and tetM) were apparently expressed in C. acetobutylicum. Our results indicate that Tn1545 is potentially useful for undertaking mutagenesis and mutational cloning in this industrially important organism. Transfer of another conjugative transposon, Tn916, from E. faecalis to C. acetobutylicum NCIB 8052 was also apparently detected. Circumstantial evidence suggests that there may be a hot spot for Tn916 insertion in the C. acetobutylicum NCIB 8052 chromosome.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Earlier efforts of transposon mutagenesis were demonstrated in C. saccharobutylicum, C. acetobutylicum, and C. beijerinckii, but issues with multiple transposon insertions per mutant and non-random distribution of insertions were reported.
“Earlier efforts of transposon mutagenesis were demonstrated in C. saccharobutylicum (Keis et al., 2001), C. acetobutylicum, and C. beijerinckii, but issues with multiple transposon insertions per mutant, and non-random distribution of insertions were also reported (Bertram and Dürre, 1989; Woolley et al., 1989).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Woolley et al., 1989
Cites
Franke, A. E., & Clewell, D. B. (1981) · Journal of Bacteriology · doi:10.1128/jb.145.1.494-502.1981 · Cited by 552 · see citing works
Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes
Kathariou, S., Metz, P., Hof, H., & Goebel, W. (1987) · Journal of Bacteriology · doi:10.1128/jb.169.3.1291-1297.1987 · Cited by 340 · see citing works
Oxygen and the Growth and Metabolism of Clostridium acetobutylicum
O'Brien, R. W., & Morris, J. G. (1971) · Journal of General Microbiology · doi:10.1099/00221287-68-3-307 · Cited by 290 · see citing works
Conjugative Transposons and the Dissemination of Antibiotic Resistance in Streptococci
Clewell, D. B., & Gawron-Burke, C. (1986) · Annual Review of Microbiology · doi:10.1146/annurev.mi.40.100186.003223 · Cited by 274 · see citing works
Gawron-Burke, C., & Clewell, D. B. (1984) · Journal of Bacteriology · doi:10.1128/jb.159.1.214-221.1984 · Cited by 262 · see citing works
A transposon in Streptococcus faecalis with fertility properties
Gawron-Burke, C., & Clewell, D. B. (1982) · Nature · doi:10.1038/300281a0 · Cited by 236 · see citing works
Transposable multiple antibiotic resistance in Streptococcus pneumoniae
Courvalin, P. M., & Carlier, C. (1986) · Molecular and General Genetics MGG · doi:10.1007/bf00430441 · Cited by 218 · see citing works
Taxonomy of the Clostridia: Ribosomal Ribonucleic Acid Homologies among the Species
Johnson, J. L., & Francis, B. (1975) · Journal of General Microbiology · doi:10.1099/00221287-88-2-229 · Cited by 213 · see citing works
An Index to Deoxyribonucleic Acid Base Compositions of Bacterial Species
Hill, L. R. (1966) · Journal of General Microbiology · doi:10.1099/00221287-44-3-419 · Cited by 185 · see citing works
Recent advances in the genetics of the clostridia
Young, M. R., Minton, N. P., & Staudenbauer, W. L. (1989) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1989.tb03402.x · Cited by 176 · see citing works
Self-citationAn intermediate in transposition of the conjugative transposon Tn916.
Scott, J. R., Kirchman, P. A., & Caparon, M. G. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.13.4809 · Cited by 152 · see citing works
Martin, P., Trieu‐Cuot, P., & Courvalin, P. M. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.17.7047 · Cited by 149 · see citing works
Christie, P. J., Korman, R. Z., Zahler, S. A., Adsit, J. C., & Dunny, G. M. (1987) · Journal of Bacteriology · doi:10.1128/jb.169.6.2529-2536.1987 · Cited by 144 · see citing works
Poyart, C., Trieu‐Cuot, P., Carlier, C., & Courvalin, P. M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08373.x · Cited by 144 · see citing works
Transferable tetracycline resistance in Clostridium difficile
Smith, C. J., Markowitz, S. M., & Macrina, F. L. (1981) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.19.6.997 · Cited by 136 · see citing works
Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation
Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P. (1988) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1988.tb03154.x · Cited by 133 · see citing works
Tn1545: A conjugative shuttle transposon
Courvalin, P. M., & Carlier, C. (1987) · Molecular and General Genetics MGG · doi:10.1007/bf00333582 · Cited by 128 · see citing works
Sequence analysis of termini of conjugative transposon Tn916
Clewell, D. B., Flannagan, S. E., Ike, Y., et al. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.7.3046-3052.1988 · Cited by 126 · see citing works
Genetic organization of the bacterial conjugative transposon Tn916
Senghas, E., Jones, J. M., Yamamoto, M., et al. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.1.245-249.1988 · Cited by 123 · see citing works
Transferable Resistance to Clindamycin, Erythromycin, and Tetracycline in Clostridium difficile
Wüst, J., & Hardegger, U. (1983) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.23.5.784 · Cited by 103 · see citing works
DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in pneumococcus
Shoemaker, N. B., Smith, M. D., & Guild, W. R. (1980) · Plasmid · doi:10.1016/s0147-619x(80)90036-0 · Cited by 102 · see citing works
Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae
Buu-Hoï, A., & Horodniceanu, T. (1980) · Journal of Bacteriology · doi:10.1128/jb.143.1.313-320.1980 · Cited by 83 · see citing works
Hächler, H., Kayser, F. H., & Berger‐Bächi, B. (1987) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.31.7.1033 · Cited by 82 · see citing works
Conjugal transfer and expression of streptococcal transposons in Clostridium acetobutylicum
Bertram, J., & D�rre, P. (1989) · Archives of Microbiology · doi:10.1007/bf00454874 · Cited by 77 · see citing works
Recent advances in the genetics of the clostridia
YOUNG, M. (1989) · FEMS Microbiology Reviews · doi:10.1016/0168-6445(89)90026-0 · Cited by 70 · see citing works
Self-citationPhysical analysis of the conjugative shuttle transposon Tn1545
Caillaud, F., Carlier, C., & Courvalin, P. M. (1987) · Plasmid · doi:10.1016/0147-619x(87)90009-6 · Cited by 66 · see citing works
Plasmid replication stimulates DNA recombination in Bacillus subtilis
Noirot, P., Petit, M., & Ehrlich, S. D. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90509-2 · Cited by 62 · see citing works
Jones, J. M., Yost, S. C., & Pattee, P. A. (1987) · Journal of Bacteriology · doi:10.1128/jb.169.5.2121-2131.1987 · Cited by 56 · see citing works
Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545
Caillaud, F., & Courvalin, P. M. (1987) · Molecular and General Genetics MGG · doi:10.1007/bf00329844 · Cited by 53 · see citing works
Conjugative transfer of the transposon Tn919to lactic acid bacteria
Hill, C., Daly, C., & Fitzgerald, G. F. (1985) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1985.tb00996.x · Cited by 48 · see citing works
Oultram, J. D., Davies, A., & Young, M. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02056.x · Cited by 31 · see citing works
Self-citationGeneration of Tn5 insertions in streptococcal conjugative transposon Tn916
Yamamoto, M., Jones, J. M., Senghas, E., et al. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.5.1069-1072.1987 · Cited by 30 · see citing works
Inter- and intrageneric transfer of Tn916 between Streptococcus faecalis and Clostridium tetani
Volk, W. A., Bizzini, B., Jones, K. R., et al. (1988) · Plasmid · doi:10.1016/0147-619x(88)90044-3 · Cited by 21 · see citing works
Magot, M. (1983) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1983.tb00468.x · Cited by 19 · see citing works
Transfection and transformation ofClostridium acetobutylicum strain N1-4081 protoplasts
Reysset, G., Hubert, J., Podvin, L., et al. (1988) · Biotechnology Techniques · doi:10.1007/bf01875765 · Cited by 17 · see citing works
CONJUGAL GENE TRANSFER IN CLOSTRIDIUM ACETOBUTYLICUM
Davies, A., Oultram, J. D., Pennock, A., et al. (1988) · Genetics and Biotechnology of Bacilli · doi:10.1016/b978-0-12-274161-6.50070-x · Cited by 6 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works
Gene transfer to Clostridium cellulolyticum ATCC 35319
Jennert, K. C. B., Tardif, C., Young, D. I., & Young, M. (2000) · Microbiology · doi:10.1099/00221287-146-12-3071 · Cited by 112 · see citing works
Self-citationZhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y. (2016) · FEMS Microbiology Letters · doi:10.1093/femsle/fnw065 · Cited by 23 · see citing works
- Authors
- Woolley, R. C., Pennock, A., Ashton, R. J., Davies, A., & Young, M.
- Affiliations
- Aberystwyth University (GB)
- Corresponding author
- Young, M.
- Venue
- Plasmid
- Publisher
- Elsevier BV
- Published
- 1989-09
- Volume
- 22
- Issue
- 2
- Pages
- 169-174
- ISSN
- 0147-619X (ISSN-L)
- OpenAlex
- W1988206245
- Cited by
- 55
- References
- 36
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 2 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plasmid (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- Tn916, conjugative transposons, Clostridium acetobutylicum, transconjugation, antibiotic resistance genes, tetM, mutagenesis, Streptococcus pneumoniae, Enterococcus faecalis, Bacillus subtilis
- MeSH terms
- Bacillus subtilis, Clostridium, Conjugation, Genetic, DNA Transposable Elements, Drug Resistance, Microbial, Enterococcus faecalis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
