Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
Open accessAbstract
Clostridium acetobutylicum is an industrially important Gram-positive organism, which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessitate the availability of additional genetics tools to facilitate the derivation of a greater understanding of the underlying metabolic and regulatory control processes in operation through forward genetic strategies. In this study, a xylose inducible, mariner-based, transposon system was developed and shown to allow high-efficient random mutagenesis in the model strain ATCC 824. Of the thiamphenicol resistant colonies obtained, 91.9% were shown to be due to successful transposition of the catP-based mini-transposon element. Phenotypic screening of 200 transposon clones revealed a sporulation-defective clone with an insertion in spo0A, thereby demonstrating that this inducible transposon system can be used for forward genetic studies in C. acetobutylicum.
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An inducible transposon system for random mutagenesis has been described for C. acetobutylicum that showed over 90% successful transposition.
“Recently, an inducible transposon system for random mutagenesis has been described for C. acetobutylicum that showed over 90% successful transposition (Zhang et al., 2016).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Zhang et al., 2016
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Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works
- Authors
- Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y.
- Affiliations
- Engineering and Physical Sciences Research Council (GB)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Zhang, Ying
- Xu, Shu
- Gu, Yang
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-03-20
- Volume
- 363
- Issue
- 8
- Pages
- fnw065
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- PMC
- PMC4941238
- OpenAlex
- W2310679544
- Cited by
- 23
- References
- 35
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- FEMS Microbiology Letters (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Repository@Nottingham (University of Nottingham) (publishedVersion, cc-by)
- Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Natural Science Foundation of Shanghai
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council (BB/G530341/1, BB/L01081X/1, BB/I004475/1, BB/G016224/1)
- Youth Innovation Promotion Association
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- Clostridium acetobutylicum, mariner transposon, random mutagenesis, xylose induction, forward genetics, Spo0A, genetic instruments
- MeSH terms
- Acetone, Ethanol, Butanols, DNA Transposable Elements, Fermentation, Spores, Bacterial, Xylose, Mutagenesis, Clostridium acetobutylicum
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