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Cited by 104Viral Infectious Diseases and Gene Expression in InsectsFluid Dynamics and MixingProtein purification and stability

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products

Kurano, N., Leist, C., Messi, F., Kurano, S., Fiechter, A.·1990·Journal of Biotechnology
DOI 10.1016/0168-1656(90)90055-g
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Abstract

Effects of biochemical factors, i.e., medium components and metabolic byproducts, on growth of Chinese hamster ovary (CHO) cells were investigated. Glucose and ammonia were found to inhibit the growth. Kinetic analysis gave the inhibition constants, 0.14 g l-1 for ammonia and 5.0 g l-1 for glucose. Since glutamine was unstable and was a main source of ammonia, precise studies on glutamine degradation and ammonia formation process were done. By evaluating the spontaneous reactions, net glutamine utilization and net ammonia production by the cells could be estimated. It became evident that asparagine could support the growth of CHO cells as a stable substitute for glutamine. Then, a glucose fed-batch culture was grown on a glutamine free and asparagine supplemented medium. Because of (1) low glucose concentration, but (2) no glucose limitation and (3) low ammonia accumulation, the maximum total cell concentration reached 3.4 x 10(6) ml-1, which was 1.8 times greater than that in the control experiment (initial 1.15 g l-1 glucose and 0.29 g l-1 glutamine, and no glucose feed).

Overview

Integrity signals

Registry

Crossref · 10.1016/0168-1656(90)90055-g

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

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Citations

Cites

Research works printed but not linked: 2

  1. Bergmeyer, 1985, Ammonia, Vol. 8, 454
  2. Lund, 1985, L-Glutamine and L-glutamate, vol. 8, 357

Cited by

See all 104

Metadata

Authors
Kurano, N., Leist, C., Messi, F., Kurano, S., & Fiechter, A.
Affiliations
ETH Zurich (CH)
Corresponding author
Kurano, N.
ORCID
Fiechter, A.
Venue
Journal of Biotechnology
Publisher
Elsevier BV
Published
1990-07
Volume
15
Issue
1-2
Pages
113-128
ISSN
0168-1656 (ISSN-L)
DOI
10.1016/0168-1656(90)90055-g
OpenAlex
W2030992249
Cited by
104
References
24
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 5 · 2020: 2 · 2019: 8 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 3
FWCI
0.98
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Fluid Dynamics and Mixing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Keywords
CHO cells, glucose inhibition, ammonia inhibition, asparagine, growth kinetics
MeSH terms
Ammonia, Animals, Asparagine, Cell Division, Cell Line, Cells, Cultured, Female, Glucose, Glutamine, Cricetinae, Kinetics, Lactates, Ovary, Oxidation-Reduction, Lactic Acid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.